LOCUS       sequence-245106-       10477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Punc4::QF2::QS::mKate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   Mao S, Qi Y, Zhu H, Huang X, Zou Y, Chi T
  TITLE     A Tet/Q Hybrid System for Robust and Versatile Control of Transgene 
            Expression in C. elegans.
  JOURNAL   iScience. 2019 Jan 25;11:224-237. doi: 10.1016/j.isci.2018.12.023. 
            Epub 2018 Dec 27.
  PUBMED    30634168
REFERENCE   2  (bases 1 to 10477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2019-01-25"; volume: "11:224-237. doi"; pages: " 
            10.1016/j.isci.2018.12.023. Epub 2018 Dec 27"
COMMENT     Sequence Label: TC690
FEATURES             Location/Qualifiers
     source          1..10477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2519..2575
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(4330..4352)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4452..4471
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4549..4567)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4635..4739
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4740..4808,4809..5600)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4958..4977)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5771..6359
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6260..6279
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             10170..10226
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gccccgccgt gcgctgccgc atcatcccat caccgccagc ttcgatcccg atgcttccat
       61 tgttatcgcc ggcattcgtg gcgctggcaa gtctacgctg gccatcatgg catctaccgc
      121 catgaagcgc aagatagtcg acctggaatc cgagttccat catcttactg gcttgtctag
      181 ttccagctac aagaagacac acggcccggt cgactatggg aggcgccaga tcgccatctt
      241 gcagaacatc ttgaatctgc acaggacccg cgccattctc gtctgctctt ggctggagcg
      301 ggatgtgcag gccatgttgc aggatttcag cgtgtctaat cctgtcattt acgttctgcg
      361 cgatgccaaa gctatcgagg cccatctgaa gggatacgac aagtccaaag ttggcaccct
      421 ccttgatgcc accagtactg tccttcgccg ctgcacccgt ttcgagttct tcaacgtctc
      481 cgaagagaac ctggacaccc actccgcttc aacatcacca cctgctgttc cggaccagcg
      541 gcataccgcg ccgtatctaa cgcttaaacg agccgagcgc cacttcctca aatttctctc
      601 cttgattcta cccaagggga ccataccttt tgtcgagtcc gcctttcccc tggcttccgt
      661 ccccgttgaa cagcgccgct ttacctacgc cctcgccttg cctgtatctg ccttgctcga
      721 caaaggcgtc gatatccaag agcttgatgt cggtgtagac gcaatcgaga tcattgtaga
      781 cgatcttgca acgagcgaat ccggcccaac gagccccttg ggtcttgcgc cccaccgagc
      841 gagcgagatc agtcgtgttg taggcgaaat caggagggac acagtgatcc ccatcattct
      901 gcacgtggtc tttccagaaa gagcgctata tgaagaagct ctgctcgcgc tttacatgac
      961 ttacctgaac catgccttaa ggcttgcgcc agattatctt acggtcgatc tggggctcga
     1021 ttctggcttg cttgggcaac taaccaccgt tcaaggaacc accaaggtca tcggcaataa
     1081 acaacttgca gaggtcaatt cgccgcgctg gggggatcca tcttggttac aagcctatga
     1141 aaaggcccag aatacaggat gcgacttggt aaggttgacc agaccggctt caaatccccg
     1201 ggacaacaca gacattcggc agttccacgt tgctgtagag gccgtcgggg gtccaaggct
     1261 cccatttatt gcttacaaca caggacgcct aggtcggaca tcgatgtgtt ttaacgagat
     1321 cctgactcca gttacaccag tgcctaccaa ggaggatgca atcgggctcc gcaatccagc
     1381 ccatcgctat ctccagcctc cgctcacggc tctggaagca acacaggctc tctactcggc
     1441 atttgtccac gacccaatga agctgtatgt ctttggcgca aatgtgggat atagcttgtc
     1501 cccagccatg cacaatgccg cactcaaggc ctgtggcatt ccacaccatt acaagcccct
     1561 ttccacagca aacatcggga ctttgcgcga ggttatcagc gatccgcagt ttgctggagc
     1621 ctcggtcggc ctgccgttta aggtggaaat catcagcctc acacactcgc tgagccggca
     1681 cgcgaaagcc atcggagccg tcaacacctt gattccggta cgacacctta ccgcggacgg
     1741 tggaataccg gacgaggtgt ccatgttcaa caatatcagc caagccggcg ctgtcagagc
     1801 tctctacggc gagaacacgg attggattgg tatccgagcc tgccttcgcc gcggtttatc
     1861 gcccgccaat gccgtgagat caacaagcac tggtcttgtc atcggcgctg gcggaatggc
     1921 tagggcagct gtctatgcca tgcttcaact gggagtcaag aagattttga tctttaaccg
     1981 aacatttgct aatgccgaga agctggttct acacttcgag aacctgttgg tcagagacgc
     2041 attgcctctg ttgagcacag ggccaagatc ccacgacaac acctgttttc acatcattcg
     2101 atctcgagac gatccgctcc cagaaaactt caaaaacccg accatgatcg tttcctgcat
     2161 accgacacac acagtggaca acacccctga ccctgaattt actgtgcctt tgcactggct
     2221 cgacaacccc actggcggca ttgtactaga actcgactac aaatgtctca catcaccctt
     2281 gctcgaacaa acacgacgcg aggctcacag aggctgggtc gcaatggatg gacttgacct
     2341 cttgccagaa caagggtttg cccaatttga actgttcacc gggcggcgag cacctcgtcg
     2401 cttgatgagg cgcgaggttt tgcgagcata cccagatgat caagcaaaat ctcataccgc
     2461 gcagttacag cctcgcctca acggaattgc aacgcaaata tctccatggg gcagtggggc
     2521 tactaacttc agcctgctga agcaggctgg agacgtggag gagaaccctg gacctatggt
     2581 ctccgagctc attaaagaaa acatgcatat gaagctctac atggagggaa ccgtcaacaa
     2641 ccaccacttc aagtgcacct ccgagggaga gggaaagcca tacgagggaa cccaaaccat
     2701 gcgtatcaag gccgtcgagg gaggaccact cccattcgcc ttcgacatcc tcgccacctc
     2761 cttcatgtac ggatccaagg taagtttaaa catatatata ctaactaacc ctgattattt
     2821 aaattttcag accttcatca accacaccca aggaatccca gacttcttca agcaatcctt
     2881 cccagaggga ttcacctggg agcgtgtcac cacctacgag gacggaggag tcctcaccgc
     2941 cacccaagac acctccctcc aagacggatg cctcatctac aacgtcaagg taagtttaaa
     3001 cagttcggta ctaactaacc atacatattt aaattttcag atccgtggag tcaacttccc
     3061 atccaacgga ccagtcatgc aaaagaagac cctcggatgg gaggcctcca ccgagaccct
     3121 ctacccagcc gacggaggac ttgagggacg tgccgacatg gccctcaagc tcgtcggagg
     3181 aggacacctc atctgcaacc tcaaggtaag tttaaacatg attttactaa ctaactaatc
     3241 tgatttaaat tttcagacca cctaccgttc caagaagcca gccaagaacc tcaagatgcc
     3301 aggagtctac tacgtcgacc gtcgtcttga gcgtatcaag gaggccgaca aggagaccta
     3361 cgtcgagcaa cacgaggtcg ccgtcgcccg ttactgcgac ctcccatcca agctcggaca
     3421 ccgttgagcg ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata ataggggccg
     3481 ctgtcatcag agtaagttta aactgagttc tactaactaa cgagtaatat ttaaattttc
     3541 agcatctcgc gcccgtgcct ctgacttcta agtccaatta ctcttcaaca tccctacatg
     3601 ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaact tctcttaatt
     3661 tctttgtttt ttagcttctt ttaagtcacc tctaacaatg aaattgtgta gattcaaaaa
     3721 tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc attaataata
     3781 attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt tacttctgat
     3841 aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca tatgttacgt
     3901 ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat gacgtcaaat
     3961 catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa gtgaaagttt
     4021 atgaaattaa ttttcctgct tttgcttttt ggggtttccc ctattgtttg tcaagatttc
     4081 gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc aagaaagatc
     4141 ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata atttgaaagt
     4201 ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa tataccaaac
     4261 ataactgttt cctactagtc ggccgtacgg gccctttcgt ctcgcgcgtt tcggtgatga
     4321 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     4381 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     4441 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     4501 accgcacaga tgcgtaagga gaaaataccg catcaggcgg ccttaagggc ctcgtgatac
     4561 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     4621 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4681 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4741 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4801 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4861 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4921 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4981 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     5041 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5101 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5161 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5221 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5281 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5341 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5401 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5461 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5521 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5581 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5641 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5701 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5761 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5821 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5881 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5941 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     6001 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6061 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6121 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6181 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6241 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6301 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6361 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6421 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgcggcc gccaacaata
     6481 taggatgctc aggagactat aattcaaaaa acccaaccca aattagcata agcttcccca
     6541 aattggaaca gtgaaatact caatcaatca ctctggcggt tttctccaca aagaacacga
     6601 aattggaaaa aggaaaagat tgatttgttt gaaggcgtga ggaaatcgaa aaaaaattgt
     6661 ttcaaaacaa atgaaatatt ttaacgaata aacccaggaa tgccactttt tggtgcccgc
     6721 ctacaaaaaa gtcaactgtt cttaaactcc tacatagttg ccctctctaa cgaatgttgc
     6781 tttttttaaa ttttcattga atttgattat cgtctgtatg cgcctactct atcagtggaa
     6841 aacatgccca tttcccacaa atttactcgt ttggagctca aaatgttaaa tggggcatca
     6901 gggaattggc atctcacaga aagtggcgga tgagttatat gtaataataa tggtggctga
     6961 agtggaaacc gcaaaaaaag gatgtgtatt tgagattttg caacggaatt tgaatcagat
     7021 acacaaaatg agagaaattt atagattctt taaaatccac ctgaagtcgg caaatttttg
     7081 aaaggtctgt tcgttcagtt tttcataagt tttatgaatt ttgatttact actgaagttc
     7141 gagaaaagcc ctttaaccat accaataaga aattatgtta actttacaag aatattaaca
     7201 aaaaattatt aaaaaactgt gttcccctgg cattatgata tttccaccgt cagttctcta
     7261 ttaaaacttc aactctacac agtttcaact ctcaaatgag ctgtccatct tttgccttca
     7321 ggaaattatg ctaatctgct gatttcgcgc actttcacca cactatttcc aaatagaagg
     7381 aggggtggtg caacacacga gacactgcaa aaaggttcag tgaagattta tctacttgtg
     7441 agatgagagc aggaggggtg ggagaagtgt gatgattgac gggatgacaa gaacagtgat
     7501 gtggtgtcga aatggaagat gcgctaattt gtggagtttt atgaaatttg aataattaca
     7561 tttataacat ctactagtta ggcgtctgtg taggtattgc ctgcctgcct acaaggcata
     7621 tgtacgaggc aggttaaacc tcctagaaag ctacggtact ttcaaaccac ataacattta
     7681 tactggtgtc ttttcgcgtc ttcttcttga acatgtagac cctcaaaaag ctaagtttta
     7741 cactgtgtag tttgctttga aatctacaaa aattggaggc acgccttctg gactttgccg
     7801 acacgcctac tttctaccta ccaactgcct acttccacag tctagcgtga actccaatac
     7861 cattttaaaa attgttttat ctcaaatttt caatttttaa atccaatttc ccagttctaa
     7921 aaaaaaaatt ttttttttaa tttaaaaact ctcatccttt ttaatgctcg ttcccattcg
     7981 gcaagacgtg taccaaacac aaaacaaatt gactggtgat ccatctcgaa tttctcgttt
     8041 caaattagtc gttgctatat atattgtctt atgtgtggaa aacgaataag aaaaattaga
     8101 gaaagatgag agagcagggg cagaatgaaa atttgcatgg aaattgaaga agatcactgc
     8161 tttttccgat gaatgatgac taaatagaat gagttgagag gaaaataatc ataattctaa
     8221 ttttaattag aaatatgata tggaaagtaa gcaaaaccac aaggcttttg ctctgaactt
     8281 atttccgaaa aaaaaagtga aactgaaagt tagaattttc aaaataaata ttgcgagagt
     8341 caaccttcag aaaaattggc gagaaatcaa atttgaattc tctgaaaatt tagttaaaat
     8401 acatatgtta tccttctttg ttttttggcc ttagcctgcc ttcaaggcga cctgagccta
     8461 tgcgggtgcc taataccgaa acacacataa tttttttttt taatctttaa atactgtaac
     8521 gagtcgcgag atccagcgag acccatagaa tatgttgagc gctgcaaaca atcgtataac
     8581 tcttgtccaa atttttccac tctgtatttt tgcaagcagt tcaacaaatg gtcatcattt
     8641 ttataaccca tatctatatt cgaatctgac ataacaaata atactaagca catcctccca
     8701 catctctttt cattcaagtt gcgcaattcc cctaataact atggatggag gggggaccga
     8761 gcccaaagat tattcaaaac gataacgact ttttcatgcg gccaacgaaa aataaaactt
     8821 ttctcatatt ctcctatttc ccatttcaag aattacatcc ccatgggacg ttgaaatcac
     8881 atcatattag tatatgttaa ttgttctttt cactggaata tcgccacgcc ccccttccgg
     8941 tggctcattg gtttcgagaa gaaggcggag ccgaagtggg ttcttctttc ttttttttgt
     9001 ggtagaagtc tagcaggatt aagctactat tccattagag gatcttcttc ttccaaaaag
     9061 tgaaaatgcc gcctaaacgc aagacactca atgccgctgc cgaagccaat gcccacgctg
     9121 atggccatgc tgatggcaat gctgatggtc atgtcgctaa cactgcagca agcagcaaca
     9181 acgcccgttt tgcggacttg accaacattg acacacccgg cctcggccct accacgacga
     9241 cgttacttgt cgagcccgct cgttcgaaac gccagagagt ctcgagggcc tgtgatcagt
     9301 gtcgagctgc acgtgaaaag tgtgatggaa tccagccggc ttgcttcccc tgtgtgtcgc
     9361 agggccggtc gtgtacctac caggccagtc ccaagaagcg aggagtccag acgggctaca
     9421 tccgcactct cgaactggct ctggcttgga tgttcgagaa cgttgcccgc agcgaggacg
     9481 ccctccacaa tcttttggtc cgtgatgctg gccagggcag cgctctcctg gtcggcaaag
     9541 actcgcctgc tgcagaacgc ctgcatgcaa gatgggcgac gagtcgagtc aacaaaagca
     9601 tcacccgtct tctctcaggt ggatctggat ctggatctgg atctggttcc ggtcgacaac
     9661 ttgagcttcc gccgactgcg actgccactg caagtatcat gccccatgtc atggaacagc
     9721 ccctgtcaac gtcaataaat ccggtcaatg atcggtttaa tggaataccg aatcctaccc
     9781 cctacaatag cgatgcggcc ctagacgcaa ttactcaaac caatgactac ggatcagtca
     9841 atacccatgg cattctcagt acatatccac cacctgccac ccacttgaac gaggcctcgg
     9901 tggcccttgc gcctggaggt gctcctccca gaccaccccc gccatacgtt gatagtacaa
     9961 cgaaccatcc tccttaccat agtaacctcg tccccatggc caatttcgga tattcgactg
    10021 tagactatga tgcgatggtg gatgatctgg catcgatcga gtacacggat gcggttgacg
    10081 ttgatccgca gttcatgacg aacctcgggt ttgtcccagg gtgtaatttt agcgatatca
    10141 acacgtatga gcaaccatgg ggcagtgggg ctactaactt cagcctgctg aagcaggctg
    10201 gagacgtgga ggagaaccct ggacctatga acaccatccc ggcacgccat gtcggggatg
    10261 tcgccgcccg cgatcctcta cctctaccgc acatatcatc ctccgtcgcc agcggcatga
    10321 agcgttcctt cgcaaccatg gccatgctct acaacgacac tggcaacagc aacgatgtcg
    10381 gtgcccatgc caggcgacca ccacgaaccc tctccaatag tcggagcact tccgcccaca
    10441 gagtacctct aggctcttgg tcggcgccca attcccc
//
