LOCUS       sequence-245115-        7715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human androgen receptor (R405A) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Lagarde WH, Blackwelder AJ, Minges JT, Hnat AT, French FS, Wilson EM
  TITLE     Androgen receptor exon 1 mutation causes androgen insensitivity by 
            creating phosphorylation site and inhibiting melanoma antigen-A11 
            activation of NH2- and carboxyl-terminal interaction-dependent 
            transactivation.
  JOURNAL   J Biol Chem. 2012 Mar 30;287(14):10905-15. doi: 
            10.1074/jbc.M111.336081. Epub 2012 Feb 13.
  PUBMED    22334658
REFERENCE   2  (bases 1 to 7715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.336081"; journalName: "J Biol Chem"; date: 
            "2012-03-30- 30"; volume: "287"; issue: "14"; pages: "10905-15"
COMMENT     Sequence Label: pCMV-AR-R405A
FEATURES             Location/Qualifiers
     source          1..7715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    3739..4361
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3938..3957)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4385..4405)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4390..4719
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4570..4705
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4757..4776)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4758..4776)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4790..4806)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4790..4806)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4803..4825)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4814..4830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4838..4844,4845..4862,4863..4868))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4883..4904
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5021..5038)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5192..5780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5272..5291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5951..6742,6743..6811))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6574..6593
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6812..6916)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6943..7398
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7030..7049)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7240..7261
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7524..7546
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7538..7555
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7539..7555
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattcaga tctgtccacc gtgtgtcttc ttctgcacga gactttgagg ctgtcagagc
      661 gctttttgcg tggttgctcc cgcaagtttc cttctctgga gcttcccgca ggtgggcagc
      721 tagctgcagc gactaccgca tcatcacagc ctgttgaact cttctgagca agagaagggg
      781 aggcggggta agggaagtag gtggaagatt cagccaagct caaggatgga agtgcagtta
      841 gggctgggaa gggtctaccc tcggccgccg tccaagacct accgaggagc tttccagaat
      901 ctgttccaga gcgtgcgcga agtgatccag aacccgggcc ccaggcaccc agaggccgcg
      961 agcgcagcac ctcccggcgc cagtttgctg ctgctgcagc agcagcagca gcagcagcag
     1021 cagcagcagc agcagcagca gcagcagcaa gagactagcc ccaggcagca gcagcagcag
     1081 cagggtgagg atggttctcc ccaagcccat cgtagaggcc ccacaggcta cctggtcctg
     1141 gatgaggaac agcaaccttc acagccgcag tcggccctgg agtgccaccc cgagagaggt
     1201 tgcgtcccag agcctggagc cgccgtggcc gccagcaagg ggctgccgca gcagctgcca
     1261 gcacctccgg acgaggatga ctcagctgcc ccatccacgt tgtccctgct gggccccact
     1321 ttccccggct taagcagctg ctccgctgac cttaaagaca tcctgagcga ggccagcacc
     1381 atgcaactcc ttcagcaaca gcagcaggaa gcagtatccg aaggcagcag cagcgggaga
     1441 gcgagggagg cctcgggggc tcccacttcc tccaaggaca attacttagg gggcacttcg
     1501 accatttctg acaacgccaa ggagttgtgt aaggcagtgt cggtgtccat gggcctgggt
     1561 gtggaggcgt tggagcatct gagtccaggg gaacagcttc ggggggattg catgtacgcc
     1621 ccacttttgg gagttccacc cgctgtgcgt cccactcctt gtgccccatt ggccgaatgc
     1681 aaaggttctc tgctagacga cagcgcaggc aagagcactg aagatactgc tgagtattcc
     1741 cctttcaagg gaggttacac caaagggcta gaaggcgaga gcctaggctg ctctggcagc
     1801 gctgcagcag ggagctccgg gacacttgaa ctgccgtcta ccctgtctct ctacaagtcc
     1861 ggagcactgg acgaggcagc tgcgtaccag agtcgcgact actacaactt tccactggct
     1921 ctggccggac cgccgccccc tccgccgcct ccccatcccc acgctcgcat caagctggag
     1981 aacccgctgg actacggcag cgcctgggcg gctgcggcgg cgcagtgcgc ctatggggac
     2041 ctggcgagcc tgcatggcgc gggtgcagcg ggacccggtt ctgggtcacc ctcagccgcc
     2101 gcttcctcat cctggcacac tctcttcaca gccgaagaag gccagttgta tggaccgtgt
     2161 ggtggtggtg ggggtggtgg cggcggcggc ggcggcggcg gcggcggcgg cggcggcggc
     2221 ggcggcggcg aggcgggagc tgtagccccc tacggctaca ctcggccccc tcaggggctg
     2281 gcgggccagg aaagcgactt caccgcacct gatgtgtggt accctggcgg catggtgagc
     2341 agagtgccct atcccagtcc cacttgtgtc aaaagcgaaa tgggcccctg gatggatagc
     2401 tactccggac cttacgggga catgcgtttg gagactgcca gggaccatgt tttgcccatt
     2461 gactattact ttccacccca gaagacctgc ctgatctgtg gagatgaagc ttctgggtgt
     2521 cactatggag ctctcacatg tggaagctgc aaggtcttct tcaaaagagc cgctgaaggg
     2581 aaacagaagt acctgtgcgc cagcagaaat gattgcacta ttgataaatt ccgaaggaaa
     2641 aattgtccat cttgtcgtct tcggaaatgt tatgaagcag ggatgactct gggagcccgg
     2701 aagctgaaga aacttggtaa tctgaaacta caggaggaag gagaggcttc cagcaccacc
     2761 agccccactg aggagacaac ccagaagctg acagtgtcac acattgaagg ctatgaatgt
     2821 cagcccatct ttctgaatgt cctggaagcc attgagccag gtgtagtgtg tgctggacac
     2881 gacaacaacc agcccgactc ctttgcagcc ttgctctcta gcctcaatga actgggagag
     2941 agacagcttg tacacgtggt caagtgggcc aaggccttgc ctggcttccg caacttacac
     3001 gtggacgacc agatggctgt cattcagtac tcctggatgg ggctcatggt gtttgccatg
     3061 ggctggcgat ccttcaccaa tgtcaactcc aggatgctct acttcgcccc tgatctggtt
     3121 ttcaatgagt accgcatgca caagtcccgg atgtacagcc agtgtgtccg aatgaggcac
     3181 ctctctcaag agtttggatg gctccaaatc accccccagg aattcctgtg catgaaagca
     3241 ctgctactct tcagcattat tccagtggat gggctgaaaa atcaaaaatt ctttgatgaa
     3301 cttcgaatga actacatcaa ggaactcgat cgtatcattg catgcaaaag aaaaaatccc
     3361 acatcctgct caagacgctt ctaccagctc accaagctcc tggactccgt gcagcctatt
     3421 gcgagagagc tgcatcagtt cacttttgac ctgctaatca agtcacacat ggtgagcgtg
     3481 gactttccgg aaatgatggc agagatcatc tctgtgcaag tgcccaagat cctttctggg
     3541 aaagtcaagc ccatctattt ccacacccag tgaagcattg gaaaccctat ttccccaccc
     3601 cagctcatgc cccctttcag atgtcttctg cctgttataa ctctgcacta ctcctctgca
     3661 gtgccttggg gaatttcctc tattgatgta cagtctgtca tgagcttgca tgcctgcagg
     3721 tcgactctag aggatcccgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc
     3781 ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt
     3841 ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag
     3901 gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt
     3961 gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg
     4021 cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg
     4081 tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct
     4141 caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct
     4201 cccttccctg tccttctgat tttaaaataa ctataccagc aggaggacgt ccagacacag
     4261 cataggctac ctggccatgc ccaaccggtg ggacatttga gttgcttgct tggcactgtc
     4321 ctctcatgcg ttgggtccac tcagtagatg cctgttgaat tgggtacgcg gccagcttgg
     4381 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     4441 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     4501 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4561 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4621 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     4681 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcctcga ggaactgaaa
     4741 aaccagaaag ttaattccct atagtgagtc gtattaaatt cgtaatcatg tcatagctgt
     4801 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4861 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     4921 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     4981 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     5041 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5101 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5161 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5221 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5281 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5341 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5401 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5461 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5521 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5581 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     5641 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5701 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5761 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5821 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5881 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     5941 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6001 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6061 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6121 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6181 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6241 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     6301 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     6361 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     6421 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6481 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6541 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     6601 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6661 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6721 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6781 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6841 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6901 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg
     6961 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     7021 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     7081 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     7141 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     7201 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     7261 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     7321 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     7381 tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     7441 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     7501 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
     7561 gctgatctat acattgaatc aatattggca attagccata ttagtcattg gttatatagc
     7621 ataaatcaat attggctatt ggccattgca tacgttgtat ctatatcata atatgtacat
     7681 ttatattggc tcatgtccaa tatgaccgcc atgtt
//
