LOCUS       sequence-24512-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
COMMENT     Sequence Label: pRK5-HA GST Rheb1
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             112..138
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cttgcttcct ccggtgtcca ctcccaggtc caactgcacc tcggttctat cgattgaatt
       61 cccgaaccga cagtcggtct cttcaccaag gccattcgcg ccaccatggg ctacccctat
      121 gatgtgcctg actacgcaga tctcaatggt ggtggtggtg ggtcgagaat gtcccctata
      181 ctaggttatt ggaaaattaa gggccttgtg caacccactc gacttctttt ggaatatctt
      241 gaagaaaaat atgaagagca tttgtatgag cgcgatgaag gtgataaatg gcgaaacaaa
      301 aagtttgaat tgggtttgga gtttcccaat cttccttatt atattgatgg tgatgttaaa
      361 ttaacacagt ctatggccat catacgttat atagctgaca agcacaacat gttgggtggt
      421 tgtccaaaag agcgtgcaga gatttcaatg cttgaaggag cggttttgga tattagatac
      481 ggtgtttcga gaattgcata tagtaaagac tttgaaactc tcaaagttga ttttcttagc
      541 aagctacctg aaatgctgaa aatgttcgaa gatcgtttat gtcataaaac atatttaaat
      601 ggtgatcatg taacccatcc tgacttcatg ttgtatgacg ctcttgatgt tgttttatac
      661 atggacccca tgtggcccgg ggngatgcnt tcccaaaatt agtttgtttt aaaaaacgta
      721 ttgaatgcta tccccacaaa ttgataagta cttgaaatcc agcangtata tagcatggcc
      781 nttgcagggc tggaagccac gttgggggtg gcgacctcct ccaa
//
