LOCUS       sequence-24513-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
COMMENT     Sequence Label: pRK5-HA GST Rheb1
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      656..665
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    669..804
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(706..725)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gtggaggtgg tgggtcgagg ggaggttctg gtggatcagg aggttcaggt ggatctgggt
       61 cgacgatgcc gcagtccaag tcccggaaga tcgcgatcct gggctaccgg tctgtgggga
      121 aatcctcatt gacgattcaa tttgttgaag gccaatttgt ggactcctac gatccaacca
      181 tagaaaacac ttttacaaag ttgatcacag taaatggaca agaatatcat cttcaacttg
      241 tagacacagc cgggcaagat gaatattcta tctttcctca gacatactcc atagatatta
      301 atggctatat tcttgtgtat tctgttacat caatcaaaag ttttgaagtg attaaagtta
      361 tccatggcaa attgttggat atggtgggga aagtacaaat acctattatg ttggttggga
      421 ataagaaaga cctgcatatg gaaagggtga tcagttatga agaagggaaa gctttggcag
      481 aatcttggaa tgcagctttt ttggaatctt ctgctaaaga aaatcagact gctgtggatg
      541 tttttcgaag gataattttg gaggcagaaa aaatggacgg ggcagcttca caagggaagt
      601 cttcgtgctc ggtgatgtga atgcggccgc taagtaagta aggatcccca gcttggccgc
      661 catggcccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
      721 atttcacaaa taaagcattt ttttnnctgc attctagttg tggtntngtc caaactcatc
      781 aatgtatctt atcatgtctg gatcngggaa ttaanttnnt g
//
