LOCUS       sequence-24516-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
COMMENT     Sequence Label: pRK5-HA GST RagD 77L
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             108..134
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcttcctccg gtgtccctcc caggtccaac tgcacctcgg ttctatcgat tgaattcccg
       61 aaccgacagt cggtctcttc accaaggcca ttcgcgccac catgggctac ccctatgatg
      121 tgcctgacta cgcagatctc aatggtggtg gtggtgggtc gagaatgtcc cctatactag
      181 gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag
      241 aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt
      301 ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa
      361 cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc
      421 caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg
      481 tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc
      541 tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg
      601 atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg
      661 acccaatgtg cctggatgcg ttcccaaaat tangtttgtt ttaaaaaacg tattgaagct
      721 atcccacaaa ttgataagta cttgaatccn nccaggtata tagcatggcc ttgcagggct
      781 ggcagccacg ttggtggggc gacatccccn aaagnggagt ggnggtcaag ggagtctgg
//
