LOCUS       sequence-24518-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
COMMENT     Sequence Label: pRK5-HA GST RagD wt
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             113..139
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gctttctctc caggtggtcc actcccaggt ccaactgcac ctcggttcta tcgattgaat
       61 tcccgaaccg acagtcggtc tcttcaccaa ggccattcgc gccaccatgg gctaccccta
      121 tgatgtgcct gactacgcag atctcaatgg tggtggtggt gggtcgagaa tgtcccctat
      181 actaggttat tggaaaatta agggccttgt gcaacccact cgacttcttt tggaatatct
      241 tgaagaaaaa tatgaagagc atttgtatga gcgcgatgaa ggtgataaat ggcgaaacaa
      301 aaagtttgaa ttgggtttgg agtttcccaa tcttccttat tatattgatg gtgatgttaa
      361 attaacacag tctatggcca tcatacgtta tatagctgac aagcacaaca tgttgggtgg
      421 ttgtccaaaa gagcgtgcag agatttcaat gcttgaagga gcggttttgg atattagata
      481 cggtgtttcg agaattgcat atagtaaaga ctttgaaact ctcaaagttg attttcttag
      541 caagctacct gaaatgctga aaatgttcga agatcgttta tgtcataaaa catatttaaa
      601 tggtgatcat gtaacccatc ctgacttcat gttgtatgac gctcttgatg tggttttata
      661 catggaccca atgtgcctgg atgcngttcc caaaaattag tttgttttaa aaaacgtatt
      721 gaaagctatc ccacaaattg ataagtactt gaaatccagc aagtatatag catggncntt
      781 gcagggctgn caagccncgt ttggtggtgn cgaccctcct ccaaangng
//
