LOCUS       sequence-24521-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
COMMENT     Sequence Label: pRK5-HA GST RagC 120L
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             111..137
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     781..803
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 ttgcttcctc caggtgtccc tcccaggtcc aactgcacct cggttctatc gattgaattc
       61 ccgaaccgac agtcggtctc ttcaccaagg ccattcgcgc caccatgggc tacccctatg
      121 atgtgcctga ctacgcagat ctcaatggtg gtggtggtgg gtcgagaatg tcccctatac
      181 taggttattg gaaaattaag ggccttgtgc aacccactcg acttcttttg gaatatcttg
      241 aagaaaaata tgaagagcat ttgtatgagc gcgatgaagg tgataaatgg cgaaacaaaa
      301 agtttgaatt gggtttggag tttcccaatc ttccttatta tattgatggt gatgttaaat
      361 taacacagtc tatggccatc atacgttata tagctgacaa gcacaacatg ttgggtggtt
      421 gtccaaaaga gcgtgcagag atttcaatgc ttgaaggagc ggttttggat attagatacg
      481 gtgtttcgag aattgcatat agtaaagact ttgaaactct caaagttgat tttcttagca
      541 agctacctga aatgctgaaa atgttcgaag atcgtttatg tcataaaaca tatttaaatg
      601 gtgatcatgt aacccatcct gacttcatgt tgtatgacgc tcttgatgtt gtttatatac
      661 atggacccaa tgtgcctgga tgcgttcccn aaattagttg gttttaaaaa acgtattgaa
      721 gctatccctg naaattgata agtacttgaa atccagcang tatatagcat ggccnttgca
      781 gggctggcaa gccacgtttg gtggtggcga cntcctccaa aggnggagng
//
