LOCUS       sequence-245258-       13222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Full-length TET1 missing zinc finger domain (585-624) used to 
            PiggyBAC rescue the function of TET1/2 DKO cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13222)
  AUTHORS   Chandru A, Bate N, Vuister GW, Cowley SM
  TITLE     Sin3A recruits Tet1 to the PAH1 domain via a highly conserved 
            Sin3-Interaction Domain.
  JOURNAL   Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.1038/s41598-018-32942-w.
  PUBMED    30279502
REFERENCE   2  (bases 1 to 13222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-10-2"; volume: "8(1):14689. doi"; pages: " 
            10.1038/s41598-018-32942-w"
COMMENT     Sequence Label: FLAG-FullLength-TET1-CXXC(24)
FEATURES             Location/Qualifiers
     source          1..13222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..63
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        516..727
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             740..1008
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             1071..1094
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(7404..7978,7979..7981,7982..7990)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7571..7588)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7798..7817
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8003..8797
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLAATGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8057..8076)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8667..8686
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8857..8877)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    8919..9040
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8956..8975)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9010..9029
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   9803..9837
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10328..10344)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10328..10344)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10341..10363)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10352..10368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10376..10382,10383..10400,10401..10406))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10421..10442
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10559..10576)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10730..11318)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10810..10829)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11489..12280,12281..12349))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12112..12131
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12350..12454)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(12480..12935)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12617..12638)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12829..12848
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13062..13084
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13076..13093
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13077..13093
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgtgta aaattgacgc
       61 atgtgtttta tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt
      121 tattatattt acacttacat actaataata aattcaacaa acaatttatt tatgtttatt
      181 tatttattaa aaaaaacaaa aactcaaaat ttcttctata aagtaacaaa acttttatga
      241 gggacagccc ccccccaaag cccccaggga tgtaattacg tccctccccc gctagggggc
      301 agcagcgagc cgcccggggc tccgctccgg tccggcgctc cccccgcatc cccgagccgg
      361 cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg
      421 cttctcgctg ctctttgagc ctgcagacac ctggggggat acggggaaaa ggcctccacg
      481 gccaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
      541 ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg
      601 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
      661 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
      721 aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg
      781 aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg
      841 aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc
      901 gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc
      961 tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc
     1021 ggcgcctact ctagagctag ccaaccatgg attacaagga tcatgacatt gactacaaag
     1081 acgatgacga caagggcggt tccatgtctc gatcccgcca tgcaaggcct tccagattag
     1141 tcaggaagga agatgtaaac aaaaaaaaga aaaacagcca actacgaaag acaaccaagg
     1201 gagccaacaa aaatgtggca tcagtcaaga ctttaagccc tggaaaatta aagcaattaa
     1261 ttcaagaaag agatgttaag aaaaaaacag aacctaaacc acccgtgcca gtcagaagcc
     1321 ttctgacaag agctggagca gcacgcatga atttggatag gactgaggtt ctttttcaga
     1381 acccagagtc cttaacctgc aatgggttta caatggcgct acgaagcacc tctcttagca
     1441 ggcgactctc ccaaccccca ctggtcgtag ccaaatccaa aaaggttcca ctttctaagg
     1501 gtttagaaaa gcaacatgat tgtgattata agatactccc tgctttggga gtaaagcact
     1561 cagaaaatga ttcggttcca atgcaagaca cccaagtcct tcctgatata gagactctaa
     1621 ttggtgtaca aaatccctct ttacttaaag gtaagagcca agagacaact cagttttgga
     1681 gccaaagagt tgaggattcc aagatcaata tccctaccca cagtggccct gcagctgaga
     1741 tccttcctgg gccactggaa gggacacgct gtggtgaagg actattctct gaagagacat
     1801 tgaatgatac cagtggttcc ccaaaaatgt ttgctcagga cacagtgtgt gctccttttc
     1861 cccaaagagc cacccccaaa gttacctctc aaggaaaccc cagcattcag ttagaagagt
     1921 tgggttcacg agtagaatct cttaagttat ctgattctta cctggatccc attaaaagtg
     1981 aacatgattg ctaccccacc tccagtctta ataaggttat acctgacttg aaccttagaa
     2041 actgcttggc tcttggtggg tctacgtctc ctacctctgt aataaaattc ctcttggcag
     2101 gctcaaaaca agcgaccctt ggtgctaaac cagatcatca agaggccttc gaagctactg
     2161 caaatcaaca ggaagtttct gataccacct ctttcctagg acaggccttt ggtgctatcc
     2221 cacatcaatg ggaacttcct ggtgctgacc cagttcatgg tgaggccctg ggtgagaccc
     2281 cagatctacc agagattcct ggtgctattc cagtccaagg agaggtcttt ggtactattt
     2341 tagaccaaca agaaactctt ggtatgagtg ggagtgttgt cccagacttg cctgtcttcc
     2401 ttcctgttcc tccaaatcca attgctacct ttaatgctcc ttccaaatgg cctgagcccc
     2461 aaagcactgt ctcatatgga cttgcagtcc agggtgctat acagattttg cctttgggct
     2521 caggacacac tcctcaatca tcatcaaact cagagaaaaa ttcattacct ccagtaatgg
     2581 ctataagcaa tgtagaaaat gagaagcagg ttcatataag cttcctgcca gctaacactc
     2641 aggggttccc attagcccct gagagaggac tcttccatgc ttcactgggt atagcccaac
     2701 tctctcaggc tggtcctagc aaatcagaca gagggagctc ccaggtcagt gtaaccagca
     2761 cagttcatgt tgtcaacacc acagtggtga ctatgccagt gccaatggtc agtacctcct
     2821 cttcttccta taccactttg ctaccgactt tggaagagga gctgaaaaag aaaccatctg
     2881 ttgttgtgcc tctggaggtt ataaaggaaa acaagaggcc ccagagggaa aagaagccca
     2941 aagttttaaa ggcagatttt gacaacaaac cagtaaatgg ccccaagtca gaatccatgg
     3001 actacagtag atgtggtcat ggggaagaac aaaaattgga attgaaccca catactgttg
     3061 aaaatgtaac taaaaatgaa gacagcatga caggcatcga ggtggagaag tggacacaaa
     3121 acaagaaatc acagttaact gatcacgtga aaggagattt tagtgccaat gtcccagaag
     3181 ctgaaaaatc gaaaaactct gaagttgaca agaaacgaac caaatctcca aaattgtttg
     3241 tacaaaccgt aagaaatggc attaaacatg tacactgttt accagctgaa acaaatgttt
     3301 catttaaaaa attcaatatt gaagaattcg gcaagacatt ggaaaacaat tcttataaat
     3361 tcctaaaaga cactgcaaac cataaaaacg ctatgagctc tgttgctact gatatgagtt
     3421 gtgatcatct caaggggaga agtaacgttt tagtattcca gcagcctggc tttaactgca
     3481 gttccattcc acattcttca cactccatca taaatcatca tgctagtata cacaatgaag
     3541 gtgatcaacc aaaaactcct gagaatatac caagtaaaga accaaaagat ggatctcccg
     3601 ttcaaccaag tctcttatcg ttaatgaaag ataggagatt aacattggag caagtggtag
     3661 ccatagaggc cctgactcaa ctctcagaag ccccatcaga gaattcctcc ccatcaaagt
     3721 cagagaagga tgaggaatca gagcagagaa cagccagttt gcttaatagc tgcaaagcta
     3781 tcctctacac tgtaagaaaa gacctccaag acccaaactt acagggagag ccaccaaaac
     3841 ttaatcactg tccatctttg gaaaaacaaa gttcatgcaa cacggtggtt ttcaatgggc
     3901 aaactactac cctttccaac tcacatatca actcagctac taaccaagca tccacaaagt
     3961 cacatgaata ttcaaaagtc acaaattcat tatctctttt tataccaaaa tcaaattcat
     4021 ccaagattga caccaataaa agtattgctc aagggataat tactcttgac aattgttcca
     4081 atgatttgca tcagttgcca ccaagaaata atgaagtgga gtattgcaac cagttactgg
     4141 acagcagcaa aaaattggac tcagatgatc tatcatgtca ggatgcaacc catacccaaa
     4201 ttgaggaaga tgttgcaaca cagttgacac aacttgcttc gataattaag atcaattata
     4261 taaaaccaga ggacaaaaaa gttgaaagta caccaacaag ccttgtcaca tgtaatgtac
     4321 agcaaaaata caatcaggag aagggcacaa tccaacagaa accaccttca agtgtacaca
     4381 ataatcatgg ttcatcatta acaaaacaaa agaacccaac ccagaaaaag acaaaatcca
     4441 ccccatcaag agatcggcgg aaaaagaagc ccacagttgt aagttatcaa gaaaatgatc
     4501 ggcagaagtg ggaaaagttg tcctatatgt atggcacaat atgcgacatt tggatagcat
     4561 cgaaatttca aaattttggg caattttgtc cacatgattt tcctactgta tttgggaaaa
     4621 tttcttcctc gaccaaaata tggaaaccac tggctcaaac gaggtccatt atgcaaccca
     4681 aaacagtatt tccaccactc actcagataa aattacagag atatcctgaa tcagcagagg
     4741 aaaaggtgaa ggttgaacca ttggattcac tcagcttatt tcatcttaaa acggaatcca
     4801 acgggaaggc attcactgat aaagcttata attctcaggt acagttaacg gtgaatgcca
     4861 atcagaaagc ccatcctttg acccagccct cctctccacc taaccagtgt gctaacgtga
     4921 tggcaggcga tgaccaaata cggtttcagc aggttgttaa ggagcaactc atgcatcaga
     4981 gactgccaac attgcctggt atctctcatg aaacaccctt accggagtca gcactaactc
     5041 tcaggaatgt aaatgtagtg tgttcaggtg gaattacagt ggtttctacc aaaagtgaag
     5101 aggaagtctg ttcatccagt tttggaacat cagaattttc cacagtggac agtgcacaga
     5161 aaaattttaa tgattatgcc atgaacttct ttactaaccc tacaaaaaac ctagtgtcta
     5221 taactaaaga ttctgaactg cccacctgca gctgtcttga tcgagttata caaaaagaca
     5281 aaggcccata ttatacacac cttggggcag gaccaagtgt tgctgctgtc agggaaatca
     5341 tggagaatag gtatggtcaa aaaggaaacg caataaggat agaaatagta gtgtacaccg
     5401 gtaaagaagg gaaaagctct catgggtgtc caattgctaa gtgggtttta agaagaagca
     5461 gtgatgaaga aaaagttctt tgtttggtcc ggcagcgtac aggccaccac tgtccaactg
     5521 ctgtgatggt ggtgctcatc atggtgtggg atggcatccc tcttccaatg gccgaccggc
     5581 tatacacaga gctcacagag aatctaaagt catacaatgg gcaccctacc gacagaagat
     5641 gcaccctcaa tgaaaatcgt acctgtacat gtcaaggaat tgatccagag acttgtggag
     5701 cttcattctc ttttggctgt tcatggagta tgtactttaa tggctgtaag tttggtagaa
     5761 gcccaagccc cagaagattt agaattgatc caagctctcc cttacatgaa aaaaaccttg
     5821 aagataactt acagagtttg gctacacgat tagctccaat ttataagcag tatgctccag
     5881 tagcttacca aaatcaggtg gaatatgaaa atgttgcccg agaatgtcgg cttggcagca
     5941 aggaaggtcg tcccttctct ggggtcactg cttgcctgga cttctgtgct catccccaca
     6001 gggacattca caacatgaat aatggaagca ctgtggtttg taccttaact cgagaagata
     6061 accgctcttt gggtgttatt cctcaagatg agcagctcca tgtgctacct ctttataagc
     6121 tttcagacac agatgagttt ggctccaagg aaggaatgga agccaagatc aaatctgggg
     6181 ccatcgaggt cctggcaccc cgccgcaaaa aaagaacgtg tttcactcag cctgttcccc
     6241 gttctggaaa gaagagggct gcgatgatga cagaggttct tgcacataag ataagggcag
     6301 tggaaaagaa acctattccc cgaatcaagc ggaagaataa ctcaacaaca acaaacaaca
     6361 gtaagccttc gtcactgcca accttaggga gtaacactga gaccgtgcaa cctgaagtaa
     6421 aaagtgaaac cgaaccccat tttatcttaa aaagttcaga caacactaaa acttattcgc
     6481 tgatgccatc cgctcctcac ccagtgaaag aggcatctcc aggcttctcc tggtccccga
     6541 agactgcttc agccacacca gctccactga agaatgacgc aacagcctca tgcgggtttt
     6601 cagaaagaag cagcactccc cactgtacga tgccttcggg aagactcagt ggtgccaatg
     6661 cagctgctgc tgatggccct ggcatttcac agcttggcga agtggctcct ctccccaccc
     6721 tgtctgctcc tgtgatggag cccctcatta attctgagcc ttccactggt gtgactgagc
     6781 cgctaacgcc tcatcagcca aaccaccagc cctccttcct cacctctcct caagaccttg
     6841 cctcttctcc aatggaagaa gatgagcagc attctgaagc agatgagcct ccatcagacg
     6901 aacccctatc tgatgacccc ctgtcacctg ctgaggagaa attgccccac attgatgagt
     6961 attggtcaga cagtgagcac atctttttgg atgcaaatat tggtggggtg gccatcgcac
     7021 ctgctcacgg ctcggttttg attgagtgtg cccggcgaga gctgcacgct accactcctg
     7081 ttgagcaccc caaccgtaat catccaaccc gcctctccct tgtcttttac cagcacaaaa
     7141 acctaaataa gccccaacat ggttttgaac taaacaagat taagtttgag gctaaagaag
     7201 ctaagaataa gaaaatgaag gcctcagagc aaaaagacca ggcagctaat gaaggtccag
     7261 aacagtcctc tgaagtaaat gaattgaacc aaattccttc tcataaagca ttaacattaa
     7321 cccatgacaa tgttgtcacc gtgtcccctt atgctctcac acacgttgcg gggccctata
     7381 accattgggt ctaagcggcc gcgcccctct ccctcccccc cccctaacgt tactggccga
     7441 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     7501 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     7561 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     7621 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     7681 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     7741 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     7801 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     7861 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     7921 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat
     7981 ggccacaacc atggcgtccg gaatgattga acaagatgga ttgcacgcag gttctccggc
     8041 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     8101 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     8161 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccgcgac
     8221 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     8281 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     8341 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     8401 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     8461 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     8521 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     8581 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     8641 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     8701 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     8761 catcgccttc tatcgccttc ttgacgagtt cttctgagtc gacaatcaac ctctggatta
     8821 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     8881 atacgctgct ttaatgcctt tgtatcatgc gttaactaaa cttgtttatt gcagcttata
     8941 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9001 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg aattgactca
     9061 aatgatgtca attagtctat cagaagctca tctggtctcc cttccggggg acaagacatc
     9121 cctgtttaat atttaaacag cagtgttccc aaactgggtt cttatatccc ttgctctggt
     9181 caaccaggtt gcagggtttc ctgtcctcac aggaacgaag tccctaaaga aacagtggca
     9241 gccaggttta gccccggaat tgactggatt ccttttttag ggcccattgg tatggctttt
     9301 tccccgtatc cccccaggtg tctgcaggct caaagagcag cgagaagcgt tcagaggaaa
     9361 gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg gctcggggat
     9421 gcggggggag cgccggaccg gagcggagcc ccgggcggct cgctgctgcc ccctagcggg
     9481 ggagggacgt aattacatcc ctgggggctt tggggggggg ctgtccctga tatctataac
     9541 aagaaaatat atatataata agttatcacg taagtagaac atgaaataac aatataatta
     9601 tcgtatgagt taaatcttaa aagtcacgta aaagataatc atgcgtcatt ttgactcacg
     9661 cggtcgttat agttcaaaat cagtgacact taccgcattg acaagcacgc ctcacgggag
     9721 ctccaagcgg cgactgagat gtcctaaatg cacagcgacg gattcgcgct atttagaaag
     9781 agagagcaat atttcaagaa tgcatgcgtc aattttacgc agactatctt tctagggtta
     9841 atctagctgc atcaggatca tatcgtcggg tcttttttcc ggctcagtca tcgcccaagc
     9901 tggcgctatc tgggcatcgg ggaggaagaa gcccgtgcct tttcccgcga ggttgaagcg
     9961 gcatggaaag agtttgccga ggatgactgc tgctgcattg acgttgagcg aaaacgcacg
    10021 tttaccatga tgattcggga aggtgtggcc atgcacgcct ttaacggtga actgttcgtt
    10081 caggccacct gggataccag ttcgtcgcgg cttttccgga cacagttccg gatggtcagc
    10141 ccgaagcgca tcagcaaccc gaacaatacc ggcgacagcc ggaactgccg tgccggtgtg
    10201 cagattaatg acagcggtgc ggcgctggga tattacgtca gcgaggacgg gtatcctggc
    10261 tggatgccgc agaaatggac atggataccc cgtgagttac ccggcgggcg cgcttggcgt
    10321 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    10381 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    10441 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    10501 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    10561 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    10621 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    10681 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    10741 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    10801 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    10861 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    10921 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    10981 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    11041 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    11101 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    11161 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    11221 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    11281 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    11341 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    11401 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    11461 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    11521 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    11581 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    11641 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    11701 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    11761 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    11821 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    11881 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    11941 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    12001 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    12061 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    12121 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    12181 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    12241 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    12301 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    12361 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    12421 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
    12481 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
    12541 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
    12601 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
    12661 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
    12721 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
    12781 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
    12841 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
    12901 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa
    12961 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    13021 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    13081 aacgacggcc agtgagcgcg cctcgttcat tcacgttttt gaacccgtgg aggacgggca
    13141 gactcgcggt gcaaatgtgt tttacagcgt gatggagcag atgaagatgc tcgacacgct
    13201 gcagaacacg cagctagatt aa
//
