LOCUS       sequence-24527-e         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
COMMENT     Sequence Label: pRK5-HA GST RagB 99L
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             101..127
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccnnngngnc nctcccggtc cnctgcncct cggttctatc gattgaattc ccgaaccgac
       61 agtcggtctc ttcaccaagg ccattcgcgc caccatgggc tacccctatg atgtgcctga
      121 ctacgcagat ctcaatggtg gtggtggtgg gtcgagaatg tcccctatac taggttattg
      181 gaaaattaag ggccttgtgc aacccactcg acttcttttg gaatatcttg aagaaaaata
      241 tgaagagcat ttgtatgagc gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt
      301 gggtttggag tttcccaatc ttccttatta tattgatggt gatgttaaat taacacagtc
      361 tatggccatc atacgttata tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga
      421 gcgtgcagag atttcaatgc ttgaaggagc ggttttggat attagatacg gtgtttcgag
      481 aattgcatat agtaaagact ttgaaactct caaagttgat tttcttagca agctacctga
      541 aatgctgaaa atgttcgaag atcgtttatg tcataaaaca tatttaaatg gtgatcatgt
      601 aacccatcct gacttcatgt tgtatgacgc tcttgatgtt gttttataca tggacccaat
      661 gtgcctggat gcgttcccca aaattagttt gttttaaaaa acgtattgaa gctatcccac
      721 aaattgatng tacttgaaat cc
//
