LOCUS       sequence-245291-        4975 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID 
            with I894A mutation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Chandru A, Bate N, Vuister GW, Cowley SM
  TITLE     Sin3A recruits Tet1 to the PAH1 domain via a highly conserved 
            Sin3-Interaction Domain.
  JOURNAL   Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.1038/s41598-018-32942-w.
  PUBMED    30279502
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-10-2"; volume: "8(1):14689. doi"; pages: " 
            10.1038/s41598-018-32942-w"
COMMENT     Sequence Label: Gal4-TET1-878-911-I894A(pL84)
FEATURES             Location/Qualifiers
     source          1..4975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             680..1120
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             1142..1162
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     intron          1393..1458
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1588..1608
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1880..2014
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1917..1936)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1971..1990
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2472..2606
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2671..2688)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2842..3430)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2922..2941)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3601..4392,4393..4461))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4224..4243
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4462..4566)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4634..4652
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4690..4712)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4812..4831
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 gaagcaagcc tcctgaaaga tgaagctact gtcttctatc gaacaagcat gcgatatttg
      721 ccgacttaaa aagctcaagt gctccaaaga aaaaccgaag tgcgccaagt gtctgaagaa
      781 caactgggag tgtcgctact ctcccaaaac caaaaggtct ccgctgacta gggcacatct
      841 gacagaagtg gaatcaaggc tagaaagact ggaacagcta tttctactga tttttcctcg
      901 agaagacctt gacatgattt tgaaaatgga ttctttacag gatataaaag cattgttaac
      961 aggattattt gtacaagata atgtgaataa agatgccgtc acagatagat tggcttcagt
     1021 ggagactgat atgcctctaa cattgagaca gcatagaata agtgcgacat catcatcgga
     1081 agagagtagt aacaaaggtc aaagacagtt gactgtatcg ccggagttcg gtaccggctc
     1141 cgaaaacctg tattttcagg gcgccctctt atcgttaatg aaagatagga gattaacatt
     1201 ggagcaagtg gtagccgccg aggccctgac tcaactctca gaagccccat cagagaattc
     1261 ctccccatga aattcgagct ccgtcggatc ctggccagct agctaggtag ctagaggatc
     1321 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
     1381 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
     1441 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
     1501 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
     1561 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
     1621 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
     1681 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
     1741 gaaaaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
     1801 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     1861 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     1921 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1981 caaactcatc aatgtatctt atcatgtctg gatcagcttc agaagatggc ggagggcctc
     2041 caacacagta attttcctcc cgactcttaa aatagaaaat gtcaagtcag ttaagcagga
     2101 agtgactaac tgaccgcagc tggccgtgcg acatcctctt ttaattagtt gctaggcaac
     2161 tgccctccag agggcagtgt ggttttgcaa gaggaagcaa aaagcctctc cacccaggcc
     2221 tagaatgttt ccacccaatc attactatga caacagctgt tttttttagt attaagcaga
     2281 ggccgggggc ccctggcctc cgcttactct ggagaaaaag aagagaggca ttgtagaggc
     2341 ttccagaggc aacttgtcaa aacaggactg cttctatttc tgtcacactg tctggccctg
     2401 tcacaaggtc cagcacctcc ataccccctt taataagcag tttgggaacg ggtgcgggtc
     2461 ttactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2521 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     2581 agtagtgagg aggctttttt ggaggctgca ttaatgaatc ggccaacgcg cggggagagg
     2641 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2701 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2761 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2821 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2881 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2941 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     3001 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     3061 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     3121 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     3181 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     3241 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     3301 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     3361 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     3421 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3481 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3541 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3601 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3661 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3721 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3781 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3841 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3901 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3961 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4021 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     4081 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     4141 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4201 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     4261 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     4321 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     4381 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     4441 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     4501 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4561 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     4621 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     4681 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     4741 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     4801 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     4861 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg
     4921 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc gcgtt
//
