LOCUS       sequence-245294-       15136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for the inducible expression of ER-targeted GFP1–9 
            fragment that can be used to detect a protein tagged with GFP10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15136)
  AUTHORS   Liu TY
  TITLE     Using Tripartite Split-sfGFP for the Study of Membrane 
            Protein-Protein Interactions.
  JOURNAL   Methods Mol Biol. 2021;2200:323-336. doi: 
            10.1007/978-1-0716-0880-7_15.
  PUBMED    33175385
REFERENCE   2  (bases 1 to 15136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2021"; volume: "2200:323-336. doi"; pages: " 
            10.1007/978-1-0716-0880-7_15"
COMMENT     Sequence Label: UBQ10:sXVE:ER-S11-GFP1–9
FEATURES             Location/Qualifiers
     source          1..15136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(28..831)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(139..158)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     749..768
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(881..1060)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4322..4368
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      5238..5490
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5657..5681
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6146..6796
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(6984..7001)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7235..7254)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            7477..7586
                     /note="incP origin of transfer"
     CDS             7619..7990
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      8117..8828
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  9450..10217
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             10409..11203
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             11502..12650
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    14246..14270
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(14517..14538)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14729..14748
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14873..14895
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14887..14904
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14888..14904
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(14917..15117)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
ORIGIN
        1 atccgtcgaa gctagcttgg gtcccgctca gaagaactcg tcaagaaggc gatagaaggc
       61 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
      121 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
      181 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
      241 cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt
      301 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
      361 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
      421 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
      481 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
      541 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
      601 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc
      661 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
      721 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
      781 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc caagctccca tgggccctcg
      841 actagagtcg agatctggat tgagagtgaa tatgagactc taattggata ccgaggggaa
      901 tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga tagtgacctt
      961 aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc attaaactcc
     1021 agaaacccgc ggctgagtgg ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa
     1081 cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc tccgctcatg
     1141 atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
     1201 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc
     1261 ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct
     1321 ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc
     1381 accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg
     1441 agtgcttgcg gcagcgtgaa gcttcgacga gtcagtaata aacggcgtca aagtggttgc
     1501 agccggcaca cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa
     1561 aaataaggca attagccaaa aacaactttg cgtgtaaaca acgctcaata cacgtgtcat
     1621 tttattatta gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt
     1681 ttcttcttct tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt
     1741 tcaatttctc aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat
     1801 ttctgtgttc cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt
     1861 atatgttctt tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat
     1921 cgttagatat catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa
     1981 taatttgagt tttgtcgaat aattactctt cgatttgtga tttctatcta gttctggtgt
     2041 tagtttctag tttgtgcgat cgaatttgtc gattaatctg agtttttctg attaacagac
     2101 tagcccgcgg aaaatgaagg ctcttactgc tagacaacaa gaagtttttg atcttattag
     2161 agatcatatt tctcaaactg gaatgcctcc tactagagct gaaattgctc aaagacttgg
     2221 atttaggtct cctaatgctg ctgaagaaca tcttaaggct cttgctagaa agggagttat
     2281 tgaaattgtt tctggagctt caagaggaat tagacttctt caagaagaag aagaaggact
     2341 tcctcttgtt ggaagagttg ctgctggaga accttcttct gctcctccta ctgatgtttc
     2401 tcttggagat gaacttcatc ttgatggaga agatgttgct atggctcatg ctgatgctct
     2461 tgatgatttt gatcttgata tgcttggaga tggagattct cctggacctg gatttactcc
     2521 tcatgattct gctccttatg gagcacttga tatggctgat tttgaatttg aacaaatgtt
     2581 tactgatgct cttggaattg atgaatatgg aggagatcct tctgctggag atatgagagc
     2641 tgctaatctt tggccttctc ctcttatgat taagaggtct aagaagaact ctcttgctct
     2701 ttctcttact gctgaccaaa tggtttctgc tcttcttgat gctgaacctc ctattcttta
     2761 ttctgaatat gatcctacta gacctttttc tgaggcttct atgatgggac ttcttactaa
     2821 tcttgctgat agagaacttg ttcatatgat taattgggct aagagagttc ctggatttgt
     2881 tgatcttact cttcatgacc aagttcatct tcttgaatgt gcttggcttg aaattcttat
     2941 gattggactt gtttggaggt ctatggaaca tcctgttaaa cttctttttg ctcctaatct
     3001 tcttcttgat agaaatcaag gaaagtgtgt tgaaggaatg gttgaaattt ttgatatgct
     3061 tcttgctact tcttcaagat ttagaatgat gaatcttcaa ggagaagaat ttgtttgtct
     3121 taagtctatt attcttctta attctggagt ttatactttt ctttcttcta ctcttaagtc
     3181 tcttgaagaa aaggatcata ttcatagagt tcttgataag attactgata ctcttattca
     3241 tcttatggct aaggctggac ttactcttca acaacaacat caaagacttg ctcaacttct
     3301 tcttattctt tctcatatta gacatatgtc taataaggga atggaacatc tttattctat
     3361 gaagtgtaag aatgttgttc ctctttatga tcttcttctt gaaatgcttg atgctcatag
     3421 acttcatgct cctacttcaa gaggaggagc ttctgttgaa gaaactgacc aatctcatct
     3481 tgctactgct ggatctactt cttctcattc tcttcaaaag tattatatta ctggagaagc
     3541 tgaaggattt cctgctactg tttgaagatc ttgatcaagc tccctggcca attcccagag
     3601 atgttagctg aaatcatcac taatcagata ccaaaatatt caaatggaaa tatcaaatag
     3661 cttctgtttc atcaaaaatg actcgaccta actgagtaag catgcttgtt cgagtattat
     3721 ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt
     3781 tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga
     3841 tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt
     3901 gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa
     3961 tcgtggcctc taatgaccga agttaatatg aggagtaaaa catcccaaac gcggccgcaa
     4021 ccggtcttgc atccagctgg gctgcaggtc gaggctaaaa aactaatcgc attatcatcc
     4081 cctcgacgta ctgtacatat aaccactggt tttatataca gcagtactgt acatataacc
     4141 actggtttta tatacagcag tcgacgtact gtacatataa ccactggttt tatatacagc
     4201 agtactgtac atataaccac tggttttata tacagcagtc gaggtaagat tagatatgga
     4261 tatgtatatg gatatgtata tggtggtaat gccatgtaat atgctcgact ctaggatctt
     4321 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca cgctgaagct
     4381 agtcgactct agccaaatct aggggatccg gcgcgcccgg gttaattaac tcgagatgaa
     4441 ggtacaggag ggtttgttcg tggtggctgt tttctacctt gcttatacgc agctagtcaa
     4501 ggggcaacct cgcaaggagt gcggatccga aaagcgtgac cacatggtcc ttcttgagta
     4561 tgtaactgct gctgggatta cagatgctag cggtggcggt tctggcggtg gtactagtat
     4621 gagaaaagga gaagaacttt tcactggagt tgtcccaatt cttattgaat tagatggtga
     4681 tgttaatggg cacaaatttt ttgtccgtgg agagggtgaa ggtgatgcaa caatcggaaa
     4741 acttagcctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
     4801 cactaccctt acctatggtg ttcaatgctt ttcaagatac ccagatcata tgaagcggca
     4861 cgacttcttc aagagcgcca tgcctgaggg atacgtgcag gagaggacca tctatttcaa
     4921 ggacgacggg acttacaaga cacgtgctga agtcaagttt gagggagaca ccctcgtcaa
     4981 caggatcgag cttaagggaa ttgatttcaa ggaggacgga aacatcctcg gccacaagtt
     5041 ggaatacaac tttaactccc acaaagtata catcacggcc gacaagcaaa ataacggcat
     5101 caaagccaac ttcacaattc gccacaacgt tgaagacggc tctgtgcaac tcgctgatca
     5161 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccacatg acgagctgta
     5221 agagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     5281 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     5341 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     5401 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     5461 cgcgcgcggt gtcatctatg ttactagatc gggaattcag atcggctgag tggctccttc
     5521 aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg
     5581 tcataacgtg actcccttaa ttctccgctc atgatcagat tgtcgtttcc cgccttcagt
     5641 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
     5701 attagaataa tcggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat
     5761 gtgcatgcca accacagggt tccccagatc tggcgccggc cagcgagacg agcaagattg
     5821 gccgccgccc gaaacgatcc gacagcgcgc ccagcacagg tgcgcaggca aattgcacca
     5881 acgcatacag cgccagcaga atgccatagt gggcggtgac gtcgttcgag tgaaccagat
     5941 cgcgcaggag gcccggcagc accggcataa tcaggccgat gccgacagcg tcgagcgcga
     6001 cagtgctcag aattacgatc aggggtatgt tgggtttcac gtctggcctc cggaccagcc
     6061 tccgctggtc cgattgaacg cgcggattct ttatcactga taagttggtg gacatattat
     6121 gtttatcagt gataaagtgt caagcatgac aaagttgcag ccgaatacag tgatccgtgc
     6181 cgccctggac ctgttgaacg aggtcggcgt agacggtctg acgacacgca aactggcgga
     6241 acggttgggg gttcagcagc cggcgcttta ctggcacttc aggaacaagc gggcgctgct
     6301 cgacgcactg gccgaagcca tgctggcgga gaatcatacg cattcggtgc cgagagccga
     6361 cgacgactgg cgctcatttc tgatcgggaa tgcccgcagc ttcaggcagg cgctgctcgc
     6421 ctaccgcgat ggcgcgcgca tccatgccgg cacgcgaccg ggcgcaccgc agatggaaac
     6481 ggccgacgcg cagcttcgct tcctctgcga ggcgggtttt tcggccgggg acgccgtcaa
     6541 tgcgctgatg acaatcagct acttcactgt tggggccgtg cttgaggagc aggccggcga
     6601 cagcgatgcc ggcgagcgcg gcggcaccgt tgaacaggct ccgctctcgc cgctgttgcg
     6661 ggccgcgata gacgccttcg acgaagccgg tccggacgca gcgttcgagc agggactcgc
     6721 ggtgattgtc gatggattgg cgaaaaggag gctcgttgtc aggaacgttg aaggaccgag
     6781 aaagggtgac gattgatcag gaccgctgcc ggagcgcaac ccactcacta cagcagagcc
     6841 atgtagacaa catcccctcc ccctttccac cgcgtcagac gcccgtagca gcccgctacg
     6901 ggctttttca tgccctgccc tagcgtccaa gcctcacggc cgcgctcggc ctctctggcg
     6961 gccttctggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7021 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7081 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7141 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7201 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7261 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7321 ctcccttcgg gaagcgtggc gcttttccgc tgcataaccc tgcttcgggg tcattatagc
     7381 gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc aaagggttcg
     7441 tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg aagtaggccc
     7501 acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg tgctcaacgg
     7561 gaatcctgct ctgcgaggct ggccggctac cgccggcgta acagatgagg gcaagcggat
     7621 ggctgatgaa accaagccaa ccaggaaggg cagcccacct atcaaggtgt actgccttcc
     7681 agacgaacga agagcgattg aggaaaaggc ggcggcggcc ggcatgagcc tgtcggccta
     7741 cctgctggcc gtcggccagg gctacaaaat cacgggcgtc gtggactatg agcacgtccg
     7801 cgagctggcc cgcatcaatg gcgacctggg ccgcctgggc ggcctgctga aactctggct
     7861 caccgacgac ccgcgcacgg cgcggttcgg tgatgccacg atcctcgccc tgctggcgaa
     7921 gatcgaagag aagcaggacg agcttggcaa ggtcatgatg ggcgtggtcc gcccgagggc
     7981 agagccatga cttttttagc cgctaaaacg gccggggggt gcgcgtgatt gccaagcacg
     8041 tccccatgcg ctccatcaag aagagcgact tcgcggagct ggtgaagtac atcaccgacg
     8101 agcaaggcaa gaccgagcgc ctttccgacg ctcaccgggc tggttgccct cgccgctggg
     8161 ctggcggccg tctatggccc tgcaaacgcg ccagaaacgc cgtcgaagcc gtgtgcgaga
     8221 caccgcggcc gccggcgttg tggatacctc gcggaaaact tggccctcac tgacagatga
     8281 ggggcggacg ttgacacttg aggggccgac tcacccggcg cggcgttgac agatgagggg
     8341 caggctcgat ttcggccggc gacgtggagc tggccagcct cgcaaatcgg cgaaaacgcc
     8401 tgattttacg cgagtttccc acagatgatg tggacaagcc tggggataag tgccctgcgg
     8461 tattgacact tgaggggcgc gactactgac agatgagggg cgcgatcctt gacacttgag
     8521 gggcagagtg ctgacagatg aggggcgcac ctattgacat ttgaggggct gtccacaggc
     8581 agaaaatcca gcatttgcaa gggtttccgc ccgtttttcg gccaccgcta acctgtcttt
     8641 taacctgctt ttaaaccaat atttataaac cttgttttta accagggctg cgccctgtgc
     8701 gcgtgaccgc gcacgccgaa ggggggtgcc cccccttctc gaaccctccc ggcccgctaa
     8761 cgcgggcctc ccatcccccc aggggctgcg cccctcggcc gcgaacggcc tcaccccaaa
     8821 aatggcagcg ctggcagtcc ttgccattgc cgggatcggg gcagtaacgg gatgggcgat
     8881 cagcccgagc gcgacgcccg gaagcattga cgtgccgcag gtgctggcat cgacattcag
     8941 cgaccaggtg ccgggcagtg agggcggcgg cctgggtggc ggcctgccct tcacttcggc
     9001 cgtcggggca ttcacggact tcatggcggg gccggcaatt tttaccttgg gcattcttgg
     9061 catagtggtc gcgggtgccg tgctcgtgtt cgggggtgcg ataaacccag cgaaccattt
     9121 gaggtgatag gtaagattat accgaggtat gaaaacgaga attggacctt tacagaatta
     9181 ctctatgaag cgccatattt aaaaagctac caagacgaag aggatgaaga ggatgaggag
     9241 gcagattgcc ttgaatatat tgacaatact gataagataa tatatctttt atatagaaga
     9301 tatcgccgta tgtaaggatt tcagggggca aggcataggc agcgcgctta tcaatatatc
     9361 tatagaatgg gcaaagcata aaaacttgca tggactaatg cttgaaacca ggacaataac
     9421 cttatagctt gtaaattcta tcataattgg gtaatgactc caacttattg atagtgtttt
     9481 atgttcagat aatgcccgat gactttgtca tgcagctcca ccgattttga gaacgacagc
     9541 gacttccgtc ccagccgtgc caggtgctgc ctcagattca ggttatgccg ctcaattcgc
     9601 tgcgtatatc gcttgctgat tacgtgcagc tttcccttca ggcgggattc atacagcggc
     9661 cagccatccg tcatccatat caccacgtca aagggtgaca gcaggctcat aagacgcccc
     9721 agcgtcgcca tagtgcgttc accgaatacg tgcgcaacaa ccgtcttccg gagactgtca
     9781 tacgcgtaaa acagccagcg ctggcgcgat ttagccccga catagcccca ctgttcgtcc
     9841 atttccgcgc agacgatgac gtcactgccc ggctgtatgc gcgaggttac cgactgcggc
     9901 ctgagttttt taagtgacgt aaaatcgtgt tgaggccaac gcccataatg cgggctgttg
     9961 cccggcatcc aacgccattc atggccatat caatgatttt ctggtgcgta ccgggttgag
    10021 aagcggtgta agtgaactgc agttgccatg ttttacggca gtgagagcag agatagcgct
    10081 gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc agtagctgaa caggagggac
    10141 agctgataga aacagaagcc actggagcac ctcaaaaaca ccatcataca ctaaatcagt
    10201 aagttggcag catcacccat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat
    10261 acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg
    10321 caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
    10381 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
    10441 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
    10501 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
    10561 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
    10621 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
    10681 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
    10741 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
    10801 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
    10861 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
    10921 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
    10981 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
    11041 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
    11101 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
    11161 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag atgtggcgca
    11221 acgatgccgg cgacaagcag gagcgcaccg acttcttccg catcaagtgt tttggctctc
    11281 aggccgaggc ccacggcaag tatttgggca aggggtcgct ggtattcgtg cagggcaaga
    11341 ttcggaatac caagtacgag aaggacggcc agacggtcta cgggaccgac ttcattgccg
    11401 ataaggtgga ttatctggac accaaggcac caggcgggtc aaatcaggaa taagggcaca
    11461 ttgccccggc gtgagtcggg gcaatcccgc aaggagggtg aatgaatcgg acgtttgacc
    11521 ggaaggcata caggcaagaa ctgatcgacg cggggttttc cgccgaggat gccgaaacca
    11581 tcgcaagccg caccgtcatg cgtgcgcccc gcgaaacctt ccagtccgtc ggctcgatgg
    11641 tccagcaagc tacggccaag atcgagcgcg acagcgtgca actggctccc cctgccctgc
    11701 ccgcgccatc ggccgccgtg gagcgttcgc gtcgtctcga acaggaggcg gcaggtttgg
    11761 cgaagtcgat gaccatcgac acgcgaggaa ctatgacgac caagaagcga aaaaccgccg
    11821 gcgaggacct ggcaaaacag gtcagcgagg ccaagcaggc cgcgttgctg aaacacacga
    11881 agcagcagat caaggaaatg cagctttcct tgttcgatat tgcgccgtgg ccggacacga
    11941 tgcgagcgat gccaaacgac acggcccgct ctgccctgtt caccacgcgc aacaagaaaa
    12001 tcccgcgcga ggcgctgcaa aacaaggtca ttttccacgt caacaaggac gtgaagatca
    12061 cctacaccgg cgtcgagctg cgggccgacg atgacgaact ggtgtggcag caggtgttgg
    12121 agtacgcgaa gcgcacccct atcggcgagc cgatcacctt cacgttctac gagctttgcc
    12181 aggacctggg ctggtcgatc aatggccggt attacacgaa ggccgaggaa tgcctgtcgc
    12241 gcctacaggc gacggcgatg ggcttcacgt ccgaccgcgt tgggcacctg gaatcggtgt
    12301 cgctgctgca ccgcttccgc gtcctggacc gtggcaagaa aacgtcccgt tgccaggtcc
    12361 tgatcgacga ggaaatcgtc gtgctgtttg ctggcgacca ctacacgaaa ttcatatggg
    12421 agaagtaccg caagctgtcg ccgacggccc gacggatgtt cgactatttc agctcgcacc
    12481 gggagccgta cccgctcaag ctggaaacct tccgcctcat gtgcggatcg gattccaccc
    12541 gcgtgaagaa gtggcgcgag caggtcggcg aagcctgcga agagttgcga ggcagcggcc
    12601 tggtggaaca cgcctgggtc aatgatgacc tggtgcattg caaacgctag ggccttgtgg
    12661 ggtcagttcc ggctgggggt tcagcagcca gcgctttact ggcatttcag gaacaagcgg
    12721 gcactgctcg acgcacttgc ttcgctcagt atcgctcggg acgcacggcg cgctctacga
    12781 actgccgata aacagaggat taaaattgac aattgtgatt aaggctcaga ttcgacggct
    12841 tggagcggcc gacgtgcagg atttccgcga gatccgattg tcggccctga agaaagctcc
    12901 agagatgttc gggtccgttt acgagcacga ggagaaaaag cccatggagg cgttcgctga
    12961 acggttgcga gatgccgtgg cattcggcgc ctacatcgac ggcgagatca ttgggctgtc
    13021 ggtcttcaaa caggaggacg gccccaagga cgctcacaag gcgcatctgt ccggcgtttt
    13081 cgtggagccc gaacagcgag gccgaggggt cgccggtatg ctgctgcggg cgttgccggc
    13141 gggtttattg ctcgtgatga tcgtccgaca gattccaacg ggaatctggt ggatgcgcat
    13201 cttcatcctc ggcgcactta atatttcgct attctggagc ttgttgttta tttcggtcta
    13261 ccgcctgccg ggcggggtcg cggcgacggt aggcgctgtg cagccgctga tggtcgtgtt
    13321 catctctgcc gctctgctag gtagcccgat acgattgatg gcggtcctgg gggctatttg
    13381 cggaactgcg ggcgtggcgc tgttggtgtt gacaccaaac gcagcgctag atcctgtcgg
    13441 cgtcgcagcg ggcctggcgg gggcggtttc catggcgttc ggaaccgtgc tgacccgcaa
    13501 gtggcaacct cccgtgcctc tgctcacctt taccgcctgg caactggcgg ccggaggact
    13561 tctgctcgtt ccagtagctt tagtgtttga tccgccaatc ccgatgccta caggaaccaa
    13621 tgttctcggc ctggcgtggc tcggcctgat cggagcgggt ttaacctact tcctttggtt
    13681 ccgggggatc tcgcgactcg aacctacagt tgtttcctta ctgggctttc tcagccccag
    13741 atctggggtc gatcagccgg ggatgcatca ggccgacagt cggaacttcg ggtccccgac
    13801 ctgtaccatt cggtgagcaa tggatagggg agttgatatc gtcaacgttc acttctaaag
    13861 aaatagcgcc actcagcttc ctcagcggct ttatccagcg atttcctatt atgtcggcat
    13921 agttctcaag atcgacagcc tgtcacggtt aagcgagaaa tgaataagaa ggctgataat
    13981 tcggatctct gcgagggaga tgatatttga tcacaggcag caacgctctg tcatcgttac
    14041 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
    14101 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
    14161 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
    14221 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
    14281 ttagacaact taataacaca ttgcggacgt ttttaatgta ctggggtggt ttttcttttc
    14341 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc
    14401 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttccgaaa
    14461 tcggcaaaat cccttataaa tcaaaagaat agcccgagat agggttgagt gttgttccag
    14521 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
    14581 tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt ttggggtcga
    14641 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
    14701 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgccattc
    14761 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    14821 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    14881 cgacgttgta aaacgacggc cagtgaatta attcccatct tgaaagaaat atagtttaaa
    14941 tatttattga taaaataaca agtcaggtat tatagtccaa gcaaaaacat aaatttattg
    15001 atgcaagttt aaattcagaa atatttcaat aactgattat atcagctggt acattgccgt
    15061 agatgaaaga ctgagtgcga tattatgtgt aatacataaa ttgatgatat agctagctta
    15121 gctcatcggg ggatcg
//
