LOCUS       sequence-245342-       11313 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid of mouse Sik3 cDNA (gatekeeper 
            mutation) with neomycin resistance gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11313)
  AUTHORS   Tarumoto Y, Lin S, Wang J, Milazzo JP, Xu Y, Lu B, Yang Z, Wei Y, 
            Polyanskaya S, Wunderlich M, Gray NS, Stegmaier K, Vakoc CR
  TITLE     Salt-Inducible Kinase inhibition suppresses acute myeloid leukemia 
            progression in vivo.
  JOURNAL   Blood. 2019 Nov 1. pii: 422697. doi: 10.1182/blood.2019001576.
  PUBMED    31697837
REFERENCE   2  (bases 1 to 11313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2019
            Nov 1. pii: 422697. doi: 10.1182/blood.2019001576."
COMMENT     Sequence Label: LentiV_Neo_Sik3_T142Q
FEATURES             Location/Qualifiers
     source          1..11313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             6285..6341
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6360..7154
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6414..6433)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7024..7043
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7170..7758
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7223..7243)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7641..7652)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7830..8063
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8141..8262
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8178..8197)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8232..8251
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8302..8437
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8457..8476)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8458..8476)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8486..8503)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8486..8502)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8495..8517)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8644..9099
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8731..8750)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8941..8962
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9125..9229
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9230..9298,9299..10090)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9448..9467)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10261..10849
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10750..10769
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11003..11020
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11137..11158
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11173..11178,11179..11196,11197..11203)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11211..11227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11216..11238
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11235..11251
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11235..11251
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11270..11290
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11272..11290
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccacccggag gtttcactgc
     2161 acaacaagat ggcggcggcg gcggcgagcg gagctggcgg ggttgccgtg gccggagcag
     2221 ggggagccgg gccggccggc cgcctgctgc ctccgcccgc cgcggggccc ccggcagccc
     2281 ccgccgcagt gcccccggcc gccgtcccgg cgcgtcccac agccccggcc tcccgcgggt
     2341 cgatggccgc tcgcatcggc tactacgaga tcgaccgcac catcggcaag ggcaacttcg
     2401 ctgtggtcaa gcgggccacg cacctcgtca ccaaggccaa ggttgctatt aaaatcatag
     2461 ataagagcca gctggatgaa gaaaacttga agaagatttt ccgggaggtt cagataatga
     2521 agatgctttg ccatccacac atcatcagac tctaccaggt catggagaca gagcgcatga
     2581 tttacctggt gcaagaatac gctagcggag gggagatatt tgaccacttg gtagcccatg
     2641 gaagaatggc agagaaggaa gctcgacgga agttcaaaca gatcgtcaca gcggtgtatt
     2701 tttgtcactg tcggaatatc gttcatcgtg atttaaaagc cgaaaactta cttctggacg
     2761 ccaatctgaa tatcaaaata gcagactttg gcttcagcaa cctcttcact ccagggcagc
     2821 tgctgaagac gtggtgtggc agccctccct atgccgcccc agagctcttc gaagggaagg
     2881 aatatgatgg gcccaaagtg gacatatgga gccttggagt tgtcctctat gtgctggtgt
     2941 gtggcgccct gccgtttgat gggagcacac tgcagaatct gcgggcccgc gtgctgagtg
     3001 gcaagttccg catcccgttc tttatgtcca cagagtgtga gcacttgatc cgccacatgc
     3061 tggtgttaga tccaaataag cgcctctcaa tggaacagat ctgcaggcac aagtggatga
     3121 agctcggaga tgcagacccc aactttgaca ggttaatagc ggagtgccag caactgaagg
     3181 aggaaaggca gtcggacccc ctcaacgatg atgtcctctt ggctatggaa gacatggggc
     3241 tggacaagga gcgcacactt cagtcattaa ggtcagatgc ctatgaccac tatagtgcaa
     3301 tctacagcct gctgtgcgat cgacacaaga aacataaaac tctgcgtccc ggagcacttc
     3361 ccagcatgcc ccaagccatg actttccagg caccagtcaa tctccaggcg gagcagacag
     3421 gcactgctat gaaccttagc gtccctcaag ttcagctgat caacccagag aaccaaatta
     3481 tagagcctga cggggctgtg aacttggaca gtgatgaggg tgaagagcct tctccagaag
     3541 ccttggttcg ctatttgtca atgaggaggc acacggtggg agtggctgac ccacgcacgg
     3601 aagttatgga agatctgcag aagctgctgc ctggctttcc tggagtaaac ccacagggtc
     3661 cattcctaca agtggcccct aacatgaact tcacgcacaa cctgttgccc atgcagagtc
     3721 tgcagccaac tgggcagctt gagtacaagg aacagtctct gttacagccg cctacactac
     3781 agctactgaa cggaatgggg ccccttggcc ggagagcctc agatggaggc gccaacatcc
     3841 aactgcatgc ccagcagctg ctcaagcgcc cacggggacc gtccccactt gtcaccatga
     3901 caccagcagt gccagcagtt acccctgtgg atgaggagag ctcggatggg gagccagatc
     3961 aggaagctgt gcagaggtac ttggcaaata ggtccaaaag gcacacactg gccatgacca
     4021 gcccgacagc tgagatccca ccggacctgc aacggcagct aggacaacag tctttccgtt
     4081 cccgggtctg gcctcctcac ctggtacctg accagcatcg ctccacctac aaggactcca
     4141 acaccctgca tctccctacg gagcgtttct cccccgtgcg ccggttctca gacggggctg
     4201 caagcatcca ggccttcaaa gctcacctgg aaaaaatggg caacagcagc agcatcaagc
     4261 agttgcagca ggagtgtgag cagctgcaga agatgtacgg ggggcaggtg gatgagagga
     4321 ccctggagaa gacccagcag cagcatatgt tgtaccagca ggagcagcac catcagatcc
     4381 tccagcagca aatccaagat tctatttgtc ctcctcagcc ctccccacct cttcaggttg
     4441 cctgtgaaaa ccaaccagcc ctcctcaccc accagctcca gaggttaagg attcaacctt
     4501 caagcccacc ccccaaccat cccagcaacc atctcttcag acagccaagt aacagtcctc
     4561 ccccggtcag cagtgccatg atcacgtctc acggtgctac gtctccctcc cagtttcaag
     4621 gcttaccctc ccatggtgca atcttccagc agcaacccga gaactgttcc ccgcctccca
     4681 gtgtagcact aacctgcctg ggcctacagc aggctagcca gtcacagcca gtgactatcc
     4741 agctacagga gccagttgac atgctcagca acatggccgg aaccgcggca ggctctgcag
     4801 ggcggagcat ccccatcagc cccagcgcca gtcagattca gatacagcac cgagccagcc
     4861 taatggctcc cttcagctat gggcaccggc ccttgtccaa gcagctgagc gctgacagtg
     4921 cagaggccca cagcttgaac atgaatcggt tctcccctgc caactacgac caggcgcatt
     4981 tacaccccca tctgttttcg gaccagtccc gaggttcccc cagcagctac agcccttcaa
     5041 caggagtggg gtttcctcca actcaagccc tgaaagttcc tccgcttgac cagttcccca
     5101 ctttccctcc cagtgcccag cagcagccac cccactatac cacgtcagca ctacagcagg
     5161 ccctgctatc tcctacaccg ccagactatc cccgacacca gcaggttccc catatccttc
     5221 aaggactgct ctctccccgg cattcactca ccggccactc ggacattcgg ctgcctccgg
     5281 cagagtttgc acagctcatc aaaaggcagc aacagcatcg acagcagcag cagcagcagc
     5341 agcagcaaca agaataccat gaattgttca ggcacatgaa ccaaggggat gctgttagcc
     5401 tagctcccag cctcggggga cagaatatga cagagcaaca ggctttatct tatcaaaatg
     5461 ctgactcgta ccaccgccac cacaccagcc cccagcatat cttacagatc agggcgcaag
     5521 attgtatctc acagggtccc tcgcccaccc ccacccatgg ctatgcccat cagccaccac
     5581 taatgcattc ggagagtatg gaggaagact gcttgtgtga ggggctcaag gagggcttcc
     5641 cagacaagag ctcaagcaca ctgaccaaag gttgccacaa cagccctctg ctcttgtgta
     5701 ccagtgggcc tggggaccct gagcctttgc tgggaactgt gagtcaggcc cgggagctgg
     5761 ggatccatcc ctacggacac cagccaactg ccaccacgtt cagtagaaat aaggtgccca
     5821 gccgagagtc tgtcctaggg aactgcctgg aaagaagttc tcctggacaa gcaatggagc
     5881 tgccggatca caacggcctt gggtacccag tacggccctt agtcagtgag cacctcaggt
     5941 cccggacgct ccagagacac cacacgatcc agaacagcga cgatgcctat gtacagctgg
     6001 ataccttgcc aggaatgagc ctggtggcag gcaaggcgct gagctctgcc cggatgtcag
     6061 atgcagttct tagtcagtct tcactcatgg gcagccaaca gtttcaggat gaggaagatg
     6121 aagaatgtgg ggtgagcctg ggccacgagc atccaggcct gggtgatggc agccagcatc
     6181 tcaactcctc tcgctatcca gctacgtgtg ttacagacat catgctcagc cacaagcacc
     6241 cagaggtctc cttcagcatg gagcaagccg gcgtgggcag cggcgccacc aacttcagcc
     6301 tgctgaagca ggccggcgac gtggaggaga accccggccc catgaccgag tacaagccca
     6361 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     6421 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     6481 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     6541 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     6601 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     6661 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     6721 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     6781 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     6841 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     6901 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     6961 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     7021 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     7081 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     7141 acgagttctt ctgaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa
     7201 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     7261 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     7321 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     7381 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     7441 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7501 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7561 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7621 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7681 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7741 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
     7801 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     7861 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7921 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7981 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     8041 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
     8101 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     8161 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8221 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     8281 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     8341 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     8401 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     8461 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     8521 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     8581 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     8641 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     8701 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     8761 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     8821 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     8881 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     8941 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     9001 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     9061 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     9121 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     9181 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     9241 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     9301 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     9361 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     9421 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     9481 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     9541 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     9601 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     9661 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     9721 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     9781 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     9841 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     9901 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     9961 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
    10021 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
    10081 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
    10141 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    10201 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    10261 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    10321 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    10381 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
    10441 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    10501 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    10561 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    10621 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    10681 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    10741 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    10801 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    10861 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    10921 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    10981 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
    11041 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
    11101 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
    11161 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
    11221 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
    11281 tcactaaagg gaacaaaagc tggagctgca agc
//
