LOCUS       sequence-24535-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
COMMENT     Sequence Label: pRK5-HA GST RagA 21L
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      98..107
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             110..136
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     779..801
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 cttgcttcct ccggtgtccc tcccaggtcc actgcacctc ggttctatcg attgaattcc
       61 cgaaccgaca gtcggtctct tcaccaaggc cattcgcgcc accatgggct acccctatga
      121 tgtgcctgac tacgcagatc tcaatggtgg tggtggtggg tcgagaatgt cccctatact
      181 aggttattgg aaaattaagg gccttgtgca acccactcga cttcttttgg aatatcttga
      241 agaaaaatat gaagagcatt tgtatgagcg cgatgaaggt gataaatggc gaaacaaaaa
      301 gtttgaattg ggtttggagt ttcccaatct tccttattat attgatggtg atgttaaatt
      361 aacacagtct atggccatca tacgttatat agctgacaag cacaacatgt tgggtggttg
      421 tccaaaagag cgtgcagaga tttcaatgct tgaaggagcg gttttggata ttagatacgg
      481 tgtttcgaga attgcatata gtaaagactt tgaaactctc aaagttgatt ttcttagcaa
      541 gctacctgaa atgctgaaaa tgttcgaaga tcgtttatgt cataaaacat atttaaatgg
      601 tgatcatgta acccatcctg acttcatgtt gtatgacgct cttgatgttg ttttatacat
      661 gggacccaaa actgcctgga tgcgttccca aaattagttt gttttaaaaa ccgtattgaa
      721 gctatcccac aaattgataa gtacttgaaa tccagcaagt atatagcatg gccttgcagg
      781 gctggcaagc cacgtttggt ggggcgacat cctcca
//
