LOCUS       sequence-245402-        8743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged BRD protein, which can be used to be expressed in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8743)
  AUTHORS   Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M,
            Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM
  TITLE     Screen identifies bromodomain protein ZMYND8 in chromatin 
            recognition of transcription-associated DNA damage that promotes 
            homologous recombination.
  JOURNAL   Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114.
  PUBMED    25593309
REFERENCE   2  (bases 1 to 8743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.252189"; journalName: "Genes Dev"; date: "2015-01-15- 
            15"; volume: "29"; issue: "2"; pages: "197-211"
COMMENT     Sequence Label: GFP-BRDT
FEATURES             Location/Qualifiers
     source          1..8743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        104..483
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        484..687
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     638..658
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     684..708
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(812..814,815..817,818..1528)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(857..878)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1118..1137)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1465..1486
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1544..1568
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4437..4461)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4469..4510
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4550..4569)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4594..4642
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4844..5272
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4931..4950)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5141..5162
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5281..5301)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5286..5615
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5466..5601
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5528..5547
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5663..5710
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5729..6127
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6285..6406
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6322..6341)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6376..6395
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6455..6471)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6455..6471)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6468..6490)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6479..6495
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6503..6509,6510..6527,6528..6533))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6548..6569
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6686..6703)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6857..7445)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6937..6956)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7616..8407,8408..8476))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8239..8258
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8477..8581)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8656..8675)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg
       61 cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact
      121 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
      181 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
      241 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
      301 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
      361 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
      421 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
      481 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
      541 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
      601 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
      661 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
      721 catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct ccggactcta
      781 gaggatccct accggtgata tcctcgagac catggtgagc aagggcgagg agctgttcac
      841 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      901 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      961 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcacct acggcgtgca
     1021 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1081 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1141 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1201 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     1261 ggtctatatc accgccgaca agcagaagaa cggcatcaag gtgaacttca agacccgcca
     1321 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1381 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa
     1441 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1501 cactctcggc atggacgagc tgtacaaggg ctcgagccca tcaacaagtt tgtacaaaaa
     1561 agcagaaggg ccgtcaaggc ccaccatgtc tctgccaagt cgacaaacag ctattattgt
     1621 taaccctcct ccaccagaat atataaatac taagaaaaat gggcgattga caaatcaact
     1681 tcagtatcta caaaaagttg tcctaaagga tttatggaag catagttttt catggccctt
     1741 tcaacgtcct gtggatgctg tgaaactaca gttgcctgat tattatacca ttataaaaaa
     1801 cccaatggat ttaaatacaa ttaagaagcg cttggagaat aaatattatg cgaaggcttc
     1861 agaatgtata gaagacttca atacaatgtt ctcaaattgt tatttatata acaagcctgg
     1921 agatgacatt gttcttatgg cacaagccct agagaagctg tttatgcaga aattatctca
     1981 gatgccacaa gaagagcaag ttgtgggtgt taaggaaaga atcaagaaag gcactcaaca
     2041 gaatatagct gtttcttctg ctaaagaaaa atcatcaccc agcgcaacag aaaaagtatt
     2101 taagcagcaa gaaattcctt ctgtatttcc taagacatct atttctccct tgaacgtggt
     2161 acagggagct tcagtcaact ccagttcaca aactgcggcc caagttacaa aaggtgtgaa
     2221 gaggaaagca gatacaacaa ctcctgcaac ttcagcagtt aaagcaagta gtgaattttc
     2281 tccaacattc acagaaaaat cagtggcact gccacctata aaagaaaata tgccaaagaa
     2341 tgttttgcca gattctcagc aacaatataa tgttgtgaag actgttaaag taactgaaca
     2401 attaaggcac tgtagtgaga ttcttaaaga aatgcttgca aagaaacatt tttcatatgc
     2461 atggcccttt tataatcctg ttgacgttaa tgctttggga ctccataact actatgacgt
     2521 tgtcaaaaat ccgatggatc ttggaactat taaggagaaa atggataacc aagaatataa
     2581 ggatgcatac aaatttgcgg cagatgttag attaatgttc atgaattgct acaagtacaa
     2641 tcctccagat cacgaagttg tgacaatggc aagaatgctt caggatgttt tcgaaacgca
     2701 tttttcaaag atcccgattg aacctgttga gagtatgcct ttatgttaca tcaaaacaga
     2761 tatcacagaa accactggta gagagaacac taatgaagcc tcctctgaag ggaactcttc
     2821 tgatgattct gaagatgagc gagttaagcg tcttgcaaag cttcaggagc agcttaaagc
     2881 tgtacatcaa cagctccagg ttttgtccca agtacctttc cgtaagctaa ataaaaagaa
     2941 agagaagtct aaaaaggaaa agaaaaaaga aaaggttaat aacagcaatg aaaatccaag
     3001 aaaaatgtgt gagcaaatga ggctaaagga aaagtccaag agaaatcagc caaagaaaag
     3061 gaaacaacag ttcattggtc taaaatctga agatgaagat aatgctaaac ctatgaacta
     3121 tgatgagaaa aggcagttaa gtctgaatat aaacaaactc cctggagata aacttgggcg
     3181 agtagttcac ataatacaat caagagagcc ttctctgagc aattccaatc ctgatgagat
     3241 agagatagac tttgaaacac tgaaagcatc aacactaaga gaattagaaa aatatgtttc
     3301 ggcatgtcta agaaagagac cattaaaacc tcctgctaag aaaataatga tgtccaaaga
     3361 agaacttcac tcacagaaaa aacaggaatt ggaaaagcgg ttactggatg ttaataatca
     3421 gttaaattct aggaaacgtc aaacaaaatc tgataaaacg caaccatcca aagctgttga
     3481 aaatgtttcc cgactgagtg agagcagcag cagcagcagc agctcatcag agtctgaaag
     3541 tagcagcagt gacttaagct cttcagacag cagtgattct gaatcagaaa tgttccctaa
     3601 gtttacagaa gtaaaaccaa atgattctcc ttctaaagag aatgtaaaga aaatgaagaa
     3661 tgaatgcata ccgcctgaag gaagaacagg cgtcacacag ataggatatt gtgtgcaaga
     3721 cacaacctct gccaatacta cccttgttca tcagaccaca ccttcacatg taatgccacc
     3781 aaatcaccac caattagcat ttaattatca agaattagaa catttacaga ctgtgaaaaa
     3841 catttcacct ttacaaattc tgcctccctc aggtgattct gaacagctct caaatggcat
     3901 aactgtgatg catccatctg gtgatagtga cacaacgatg ttagaatctg aatgtcaagc
     3961 tcctgtacag aaggatataa agattaagaa tgcagattca tggaaaagtt taggcaaacc
     4021 agtgaaacca tcaggtgtaa tgaaatcctc agatgagctc ttcaaccaat ttagaaaagc
     4081 agccatagaa aaggaagtaa aagctcggac acaggaactc atacggaagc atttggaaca
     4141 aaatacaaag gaactaaaag catctcaaga aaatcagagg gatcttggga atggattgac
     4201 tgtagaatct ttttcaaata aaatacaaaa caagtgctct ggagaagagc agaaagaaca
     4261 tcagcagtca tcagaagctc aagataaatc caaactctgg cttctcaaag accgtgattt
     4321 agcaaggcag aaagaacaag agaggaggag gagagaagca atggtgggta ccattgatat
     4381 gacccttcaa agtgacatta tgacaatgtt tgaaaacaac tttgatggcc tcatgggccc
     4441 agctttcttg tacaaagtgg ttgataacgg gaagcctatc cctaaccctc tcctcggtct
     4501 cgattctacg cgtaccggtt agtaatgagt ttaaacgggg gaggctaact gaaacacgga
     4561 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     4621 acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     4681 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     4741 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     4801 gccatagcag atctgcgcag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     4861 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     4921 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     4981 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5041 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5101 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5161 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5221 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     5281 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5341 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     5401 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     5461 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     5521 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     5581 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     5641 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     5701 ataatacgac aaggtgagga actaaaccat ggccaagcct ttgtctcaag aagaatccac
     5761 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     5821 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     5881 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     5941 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     6001 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     6061 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     6121 gggctaagca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
     6181 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     6241 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     6301 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6361 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     6421 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     6481 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6541 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6601 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6661 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6721 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6781 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6841 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6901 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6961 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7021 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7081 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7141 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7201 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7261 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7321 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7381 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7441 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7501 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7561 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7621 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7681 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7741 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7801 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7861 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7921 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7981 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8041 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8101 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8161 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8221 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8281 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8341 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8401 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8461 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8521 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8581 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     8641 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     8701 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa att
//
