LOCUS       sequence-245408-       10271 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10271)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 10271)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: GRM5-Tango
FEATURES             Location/Qualifiers
     source          1..10271
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4829..5443,5444..5830)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="RLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKN
                     KRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPT
                     EKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDS
                     MPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIEG
                     LLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHADA
                     LDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(4896..4915)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(5862..5879)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5868..6092
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6138..6566
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6225..6244)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6435..6456
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6575..6595)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6580..6909
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6760..6895
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6822..6841
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6976..7770
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7030..7049)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7640..7659
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7944..8065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7981..8000)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8035..8054
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8114..8130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8114..8130)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8127..8149)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8138..8154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8162..8168,8169..8186,8187..8192))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8207..8228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8345..8362)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8516..9104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8596..8615)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9275..10066,10067..10135))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9898..9917
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10136..10240)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
      961 agatatccag cacagtggcg gccgcgccac catgaagacg atcatcgccc tgagctacat
     1021 cttctgcctg gtattcgccg actacaagga cgatgatgac gccagcatcg atatggtgct
     1081 gttgctgatc ctttcagtgc tcctgctgaa ggaagacgta cggggctccg cccagtccag
     1141 cgagcgacgg gtggtggctc atatgccagg agacatcata atcggggcat tgttcagcgt
     1201 ccatcaccag ccaactgtcg acaaggtcca tgagcgaaaa tgcggcgcgg tacgcgagca
     1261 gtacggcatt caaagggtgg aggccatgtt gcacacactg gagagaatta acagcgatcc
     1321 taccttgctg cctaacatta cattgggatg cgaaatccgc gattcctgct ggcacagcgc
     1381 agtggctctg gagcaaagca ttgaatttat ccgggatagt ttgatcagca gcgaagagga
     1441 ggagggactg gtacgatgcg ttgatggtag cagcagctcc ttcaggtcta agaaacccat
     1501 cgtaggggtt atcgggcctg gatcctcctc cgttgcgatc caagttcaga acctgctgca
     1561 actgtttaat attccccaga ttgcttatag tgcaacttcc atggacttgt ccgataagac
     1621 cctttttaag tatttcatgc gggtagttcc aagcgacgct cagcaggcaa gggccatggt
     1681 ggatatcgtg aagcgctaca actggactta tgtcagtgct gtgcatactg agggcaatta
     1741 tggggagtcc ggaatggaag cattcaagga catgtctgct aaggagggaa tctgcatcgc
     1801 ccacagctac aagatctata gtaacgctgg ggagcagtcc tttgataagt tgctgaaaaa
     1861 gctcactagc caccttccca aggcccgagt tgtcgcctgc ttctgcgagg gaatgacagt
     1921 tcggggcttg ctcatggcta tgcggaggct ggggttggca ggagaattcc tgctgctcgg
     1981 atcagatggt tgggccgata gatacgacgt cacagatggc taccaaaggg aagcagttgg
     2041 aggcattacc atcaagctgc agtctcctga tgtgaagtgg ttcgacgact actacttgaa
     2101 gctgaggccc gagacaaatc acagaaaccc atggttccaa gagttttggc aacacagatt
     2161 tcaatgcagg ctggaaggct tcccacagga aaattccaag tacaataaga cctgtaactc
     2221 atcactgacg ttgaaaactc accatgtaca ggattcaaag atgggattcg tgattaatgc
     2281 tatctactct atggcctatg gcctccacaa tatgcaaatg agcttgtgtc ccggctacgc
     2341 aggcctctgt gacgcaatga aacccataga tgggcgaaag ttgctcgaaa gtctgatgaa
     2401 gactaacttc accggagtgt ccggcgatac aatcctgttt gacgaaaacg gtgattctcc
     2461 tggacgctac gagatcatga acttcaaaga gatgggcaag gattattttg attatatcaa
     2521 cgttgggagt tgggataacg gcgaattgaa aatggacgat gatgaggtgt ggagcaaaaa
     2581 gtccaacatc atccggagcg tgtgctccga accatgcgag aaggggcaga ttaaggtgat
     2641 aaggaaggga gaggtgagtt gctgttggac ttgcacccct tgcaaagaga acgagtacgt
     2701 ctttgacgaa tatacctgca aggcttgcca gctggggtcc tggcctacgg atgatttgac
     2761 cggatgcgat ctgatccccg tgcagtatct gagatggggc gacccagagc ccattgcagc
     2821 ggtggttttc gcttgtctcg gcctgcttgc aactctgttc gtgactgttg tattcatcat
     2881 ttaccgagat acccccgtgg ttaagagcag ttctagagaa ctgtgctaca ttatcctggc
     2941 cggcatatgc cttggttatc tgtgtacgtt ctgcctcatt gccaaaccaa aacagatcta
     3001 ttgctatctg cagcgcattg ggatcggcct gtctcccgcc atgtcctatt cagcgctcgt
     3061 gaccaaaacc aatcgaatcg cgaggatcct ggcagggtct aagaaaaaaa tatgtaccaa
     3121 gaaaccaaga tttatgtccg cctgtgccca gctcgtgatc gccttcatcc tgatctgtat
     3181 ccagcttggc attatagttg ccctttttat aatggagccc ccagacatca tgcatgacta
     3241 cccttccatc agggaagtgt acctcatctg caatactacc aatctggggg tggtaacccc
     3301 actgggatat aatggcctcc tgatcctgag ctgtaccttt tacgcgttta agacgcgcaa
     3361 tgtgcccgcg aattttaatg aggccaaata tattgctttt accatgtata cgacttgtat
     3421 aatctggctg gccttcgtgc caatctattt tggatccaat tataagataa ttaccatgtg
     3481 ctttagcgtt tccttgagcg ccaccgtggc actcggatgc atgttcgttc ctaaggtata
     3541 tattattctg gctaaaccag agcgcaatgt ccgcagcgct ttcacgacat ctaccgtcgt
     3601 taggatgcat gtgggagatg gaaagtcaag cagtgcagcc agtaggagca gcagcctggt
     3661 gaatctgtgg aaacggcgag ggagtagtgg ggaaaccctg agatacaagg acagaaggct
     3721 cgctcaacat aaaagcgaga tagaatgttt cacccccaag gggagcatgg gtaacggagg
     3781 acgcgctact atgtcatctt caaatggaaa gtccgtgact tgggcccaaa atgaaaaaag
     3841 tagccgcgga caacacctgt ggcagcgcct gtcaattcat atcaataaga aggagaaccc
     3901 caaccagacc gctgtgatca aaccatttcc caagagtaca gagtcacggg ggctgggagc
     3961 tggcgccgga gccggaggaa gtgcgggcgg cgtgggggcc acgggaggtg ctggatgcgc
     4021 cggggccgga ccaggggggc ccgagtcccc agatgcgggc cccaaagctc tgtacgacgt
     4081 ggctgaggct gaagagcact tccccgctcc tgctcggcca agaagcccct ccccaatttc
     4141 aaccttgtca catcgggccg ggtcagcctc aagaaccgat gatgatgttc ctagtcttca
     4201 ttccgaaccc gtggctcgaa gctcctcctc tcagggcagc ctgatggagc agataagctc
     4261 cgtggtgaca agattcaccg ccaacatttc agaattgaac agtatgatgc tgtcaaccgc
     4321 cgcacccagt cctggtgttg gcgcacctct ctgcagtagc tatctgatcc ccaaggaaat
     4381 ccagctgcct actactatga ccaccttcgc tgagatccag cctctgccag ccattgaagt
     4441 gactggcggg gcgcaaccag ctgcaggcgc tcaggctgct ggcgacgcgg ccagggaaag
     4501 tccagccgct ggacctgaag ccgcagcggc taagcctgat ctcgaagagt tggtcgccct
     4561 gaccccgcca agcccattcc gggactccgt ggactccggt agtactactc ccaactcccc
     4621 agtcagtgag tccgcacttt gtattccaag ctcacccaag tacgacactc tgattattcg
     4681 ggattacacg cagtcctcct ccagcctgat cgataccggt ggacgcaccc cacccagcct
     4741 gggtccccaa gatgagtcct gcaccaccgc cagctcctcc ctggccaagg acacttcatc
     4801 gaccggtgag aacctgtact tccagctaag attagataaa agtaaagtga ttaacagcgc
     4861 attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa
     4921 gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga
     4981 cgccttagcc attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga
     5041 aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca
     5101 tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct
     5161 cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc
     5221 actcagcgct gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt
     5281 cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc
     5341 tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat
     5401 catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tccgcgtaca gccgcgcgcg
     5461 tacgaaaaac aattacgggt ctaccatcga gggcctgctc gatctcccgg acgacgacgc
     5521 ccccgaagag gcggggctgg cggctccgcg cctgtccttt ctccccgcgg gacacacgcg
     5581 cagactgtcg acggcccccc cgaccgatgt cagcctgggg gacgagctcc acttagacgg
     5641 cgaggacgtg gcgatggcgc atgccgacgc gctagacgat ttcgatctgg acatgttggg
     5701 ggacggggat tccccgggtc cgggatttac cccccacgac tccgccccct acggcgctct
     5761 ggatatggcc gacttcgagt ttgagcagat gtttaccgat gcccttggaa ttgacgagta
     5821 cggtgggtag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     5881 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     5941 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     6001 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     6061 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     6121 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6181 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     6241 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     6301 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     6361 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     6421 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     6481 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     6541 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     6601 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6661 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6721 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     6781 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6841 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     6901 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
     6961 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     7021 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     7081 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     7141 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     7201 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     7261 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     7321 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     7381 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     7441 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     7501 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     7561 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     7621 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     7681 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     7741 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     7801 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     7861 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     7921 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     7981 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     8041 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     8101 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     8161 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     8221 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     8281 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     8341 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     8401 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     8461 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     8521 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8581 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8641 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8701 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8761 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8821 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8881 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8941 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9001 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9061 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9121 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9181 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9241 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9301 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9361 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9421 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9481 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9541 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9601 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9661 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9721 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9781 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9841 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9901 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9961 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10021 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10081 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10141 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    10201 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    10261 cacctgacgt c
//
