LOCUS       sequence-245416-        9868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multipartite assembly obtained by combining FBparts FB026+FB009 into
            pDGB3omega1. Used for fluorescent tagging of fungi (G418 resistance 
            + YFP), according to FungalBraid modular DNA assembly for ATMT..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Hernanz-Koers M, Gandia M, Garrigues S, Manzanares P, Yenush L, 
            Orzaez D, Marcos JF
  TITLE     FungalBraid: A GoldenBraid-based modular cloning platform for the 
            assembly and exchange of DNA elements tailored to fungal synthetic 
            biology.
  JOURNAL   Fungal Genet Biol. 2018 Jul;116:51-61. doi: 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20.
  PUBMED    29680684
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol."; date: "2018-07"; volume: "116:51-61. doi"; pages: " 
            10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20"
COMMENT     Sequence Label: FB039
FEATURES             Location/Qualifiers
     source          1..9868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(316..567)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(358..379)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(514..1026,1027..1029,1030..1032))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     707..726
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     966..987
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             2136..2930
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2190..2209)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2800..2819
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    3230..3254
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4555..5184
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5613..6686
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6752..6946
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7182..7204)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7290..7430
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7345..7364
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7445..7462)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7616..8204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7696..7715)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8292..9083)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tggcaggata tattgtggtg taaacataac ggatccggtc tcaggagagc gcatgtctca
       61 gacggtcgat gcattgcaga tgagctgtat ctggaagagg taaacccgaa acgcgtttta
      121 ttcttgttga catggagcta ttaaatcact agaaggcact ctttgctgct tggacaaatg
      181 aacgtatctt atcgagatcc tgaacaccat ttgtctcaac tccggagctg acatcgacac
      241 caacgatctt atatccagat tcgtcaagct gtttgatgat ttcagtaacg ttaagtaagc
      301 tcagctcatg actgacttgt agagctcgtc catgccgaga gtgatcccgg cggcggtcac
      361 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
      421 gtagctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
      481 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
      541 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
      601 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
      661 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
      721 gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
      781 gaagtcgtgc tgcttcatgt ggtcggggta gcgggcgaag cacttcaggc cgtagccgaa
      841 ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga tgaacttcag
      901 ggtcagcttg ccgtaggtgg cgtcgccctc gccctcgccg gacacgctga acttgtggcc
      961 gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca gctcttcacc
     1021 cttgctcacc attttaaagg ttcttggatg ggaagatgaa tatactgaag atgggaaaag
     1081 aaagagaaaa gaaaagagca gctggtgggg agagcaggaa aatatggcaa caaatgttgg
     1141 actgacgcaa cgaccttgtc aaccccgccg acacaccggg cggacagacg gggcaaagct
     1201 gcctaccagg gactgaggga cctcagcagg tcgagtgcag agcaccggat gggtcgactg
     1261 ccagcttgtg ttcccggtct gcgccgctgg ccagctcctg agcggccttt ccggtttcat
     1321 acaccgggca aagcaggaga ggcacgatat ttggacgccc tacagatgcc ggatgggcca
     1381 attagggagc ttacgcgccg ggtactcgct ctacctactt cggagaaggt actatctcgt
     1441 gaatctttta ccagatcgga agcaattgga cttctgtacc taggttaatg gcatgctatt
     1501 tcgccgacgg ctatacaccc ctggcttcac attctccttc gcttactgcc ggtgattcga
     1561 tgaagctcca tattctccga tgatgcaata gattcttggt caacgagggg cacaccagcc
     1621 tttccacttc ggggcggagg ggcggccggt cccggattaa taatcatcca ctgcacctca
     1681 gagccgccag agctgtctgg cgcagtggcg cttattactc agcccttctc tctgcgtccg
     1741 tccgtctgtc cgcatgcgcc actccgtcag gagaactgat attgaaggag cattttttgg
     1801 gcttggctgg agctagtgga ggtcaacaat gaatgcctat tttggtttag tcgtccaggc
     1861 ggtgagcaca aaatttgtgt cgtttgacaa gatggttcat ttaggcaact ggtcagatca
     1921 gccccacttg tagcagtagc ggcggcgctc gaagtgtgac tcttattagc agacaggaac
     1981 gaggacatta ttatcatctg ctgcttggtg cacgataact tggtgcgttt gtcaagcaag
     2041 gtaagtggac gacccggtca taccttctta agttcgccct tcctcccttt atttcagatt
     2101 caatctgact tacctattct acccaagcat ccaaaatgat tgaacaagat ggattgcacg
     2161 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     2221 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     2281 tcaagaccga cctgtccggt gccctgaatg aactccaaga cgaggcagcg cggctatcgt
     2341 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     2401 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     2461 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     2521 ctacctgccc attcgaccac caagcgaaac accgcatcga gcgagcacgt actcggatgg
     2581 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     2641 aactgttcgc caggctcaag gcgcggatgc ccgacggcga ggatctcgtc gtgacccacg
     2701 gcgacgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     2761 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     2821 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     2881 ccgattcgca gcgcatagcc ttctatcgcc ttcttgacga gttcttctga gcttcatcat
     2941 tccaccatca ttccactcaa cattcaggct cctctgcgca cgtaaagtgc caaaggcaat
     3001 accctgctcg gtggaatgcc gccgggcttg tcgattttac gcacatatgc gcattcttga
     3061 cttgaagcgg aggagttctt cgttgcgggt tacagtgttt taataaaaga atggtcaaat
     3121 caaactgcta gatatacctg tcagacactc tagttgttga cccctatact cttaatacat
     3181 cagacagtac atgcatgttg catgatgacg ctgtcatgag accggatcct gacaggatat
     3241 attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg
     3301 cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc
     3361 gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt
     3421 cagtgcagcc gtcatctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg
     3481 ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata
     3541 cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg
     3601 gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg
     3661 cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg
     3721 agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag
     3781 accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg
     3841 gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca
     3901 tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca
     3961 cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc
     4021 tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga
     4081 aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca
     4141 gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga
     4201 ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc
     4261 gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat
     4321 cgcggccggg tacgtgttcg agccgcccgc gcacctctca accgtgcggc tgcatgaaat
     4381 cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga
     4441 aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc
     4501 ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag
     4561 gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta
     4621 gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc
     4681 agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac
     4741 cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac
     4801 ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg
     4861 attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt
     4921 aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg
     4981 atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc
     5041 attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca
     5101 accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct
     5161 gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa
     5221 acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc
     5281 tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca
     5341 agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca
     5401 tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct
     5461 aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg
     5521 tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgcctgccg gccctgcaat
     5581 ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg
     5641 tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg
     5701 cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg
     5761 atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc
     5821 cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc
     5881 gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag
     5941 ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg
     6001 ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg
     6061 aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac
     6121 acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc
     6181 tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg
     6241 ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga
     6301 tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt
     6361 accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt
     6421 tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg
     6481 cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca
     6541 agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt
     6601 tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg
     6661 agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag
     6721 caggggaaaa aggtcgaaaa ggactctttc ctgtggatag cacgtacatt gggaacccaa
     6781 agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc
     6841 ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact
     6901 ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc
     6961 gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg
     7021 ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag
     7081 gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc
     7141 aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     7201 ccggtgacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     7261 gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
     7321 ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     7381 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg
     7441 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7501 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7561 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7621 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7681 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7741 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7801 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7861 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7921 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7981 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8041 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8101 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8161 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8221 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc
     8281 attctaggtg attatttgcc gactaccttg gtgatctcgc ctttcacgta gtggacaaat
     8341 tcttccaact gatctgcgcg cgaggccaag cgatcttctt cttgtccaag ataagcctgt
     8401 ctagcttcaa gtatgacggg ctgatactgg gccggcaggc gctccattgc ccagtcggca
     8461 gcgacatcct tcggcgcgat tttgccggtt actgcgctgt accaaatgcg ggacaacgta
     8521 agcactacat ttcgctcatc accagcccag tcgggcggcg agttccatag cgttaaggtt
     8581 tcatttagcg cctcaaatag atcctgttca ggaaccggat caaagagttc ctccgccgct
     8641 ggacctacca aggcaacgct atgttctctt gcttttgtca gcaagatagc cagatcaatg
     8701 tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat tgcgctgcca ttctccaaat
     8761 tgcagttcgc gcttagctgg ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg
     8821 acttctacag cgcggagaat ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg
     8881 ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg tcaccgtaac cagcaaatca
     8941 atatcactgt gtggcttcag gccgccatcc actgcggagc cgtacaaatg tacggccagc
     9001 aacgtcggtt cgagatggcg ctcgatgacg ccaactacct ctgatagttg agtcgatact
     9061 tcggcgatca ccgcttccct cataatgttt aactttgttt tagggcgact gccctgctgc
     9121 gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc
     9181 ttggatgccc gaggcataga ctgtacccca aaaaaacagt cataacaagc catgaaaacc
     9241 gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc
     9301 atacgctact tgcattacag cttacgaacc gaacaggctt atgtccactg ggttcgtgcc
     9361 ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gtagcagcga agtcgaggca
     9421 tttctgtcct ggctggaaca gaacttatta tttccttcct cttttctaca gtatttaaag
     9481 ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct tgcattctaa
     9541 aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc gtataacata
     9601 gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt
     9661 tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg
     9721 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
     9781 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
     9841 gccggtcggg gagctgttgg ctggctgg
//
