LOCUS       sequence-245421-        2144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part (medium-strength Mutalik library 
            promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental 
            Documents for annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2144)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P33m
FEATURES             Location/Qualifiers
     source          1..2144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     33..50
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    56..76
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     misc_feature    142..163
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      166..209
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(213..231)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        299..403
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(404..472,473..1264)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(622..641)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1435..2023
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1924..1943
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcctgca
       61 gcggccgcta ctagtaggtc tctagtatcc acaaacacta agagccggat gattaattgt
      121 caactcctga gacccactag tctctagaag cggccgcgaa ttccagaaat catccttagc
      181 gaaagctaag gatttttttt atctgaaatt ctgcctcgtg atacgcctat ttttataggt
      241 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      301 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      361 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      421 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      481 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      541 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      601 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      661 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      721 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      781 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
      841 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
      901 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
      961 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1021 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1081 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tcgcgcggta tcattgcagc
     1141 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1201 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1261 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1321 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1381 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1441 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1501 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1561 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     1621 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1681 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1741 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1801 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1861 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     1921 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     1981 tcgatttttg tgatgctcgt caggggggcg gagcctgtgg aaaaacgcca gcaacgcggc
     2041 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2101 ccctgattct gtggataacc gtattaccgc ctttgagtga gctg
//
