LOCUS       sequence-245422-        2819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for Cerulean (modified CFP; 
            variant of https://www.fpbase.org/protein/cerulean/) - Please see 
            Supplemental Documents for annotated Genbank file.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2819)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C13m
FEATURES             Location/Qualifiers
     source          1..2819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..38
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(42..85)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    88..109
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             125..838
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             125..838
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(155..183)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     778..798
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    850..870
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(876..893)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1047..1635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1127..1146)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1806..2597,2598..2666))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2429..2448
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2667..2771)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc
       61 cttagctttc gctaaggatg atttctggaa ttcgcggccg cttctagaga ctagtgggtc
      121 tcaaatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
      181 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
      241 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
      301 acttgtcact actttgactt ggggtgttca atgctttgct agatacccag atcatatgaa
      361 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
      421 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
      481 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
      541 caaattggaa tacaacgcta tttcagataa tgtatacatc actgcagaca aacaaaagaa
      601 tggaatcaaa gctaatttca aaattagaca caacattgaa gatggaagcg ttcaactagc
      661 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
      721 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag atcacatggt
      781 ccttcttgag tttgtaacag ctgctgggat tacactaggc atggatgaac tatacaaaag
      841 gtagagacct actagtagcg gccgctgcag gcttcctcgc tcactgactc gctgcgctcg
      901 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      961 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1021 cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga cgagcatcac
     1081 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1141 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1201 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1261 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1321 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1381 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1441 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1501 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1561 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1621 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1681 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1741 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1801 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     1861 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     1921 ggccccagtg ctgcaatgat accgcgcgac ccacgctcac cggctccaga tttatcagca
     1981 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2041 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     2101 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     2161 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     2221 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     2281 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     2341 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     2401 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     2461 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     2521 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     2581 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     2641 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     2701 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2761 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattat
//
