LOCUS       sequence-245423-        2752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for RhlI (quorum-sensing synthase
            for 3OC14). Please see Supplemental Documents for annotated Genbank 
            file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2752)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C36m
FEATURES             Location/Qualifiers
     source          1..2752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..31
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(35..78)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    81..102
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    776..796
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(802..819)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(973..1561)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1053..1072)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1732..2523,2524..2592))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2355..2374
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2593..2697)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa atccttagct
       61 ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agactagtgg gtctcaaatg
      121 atcgaattgc tctctgaatc gctggaaggg ctttccgccg ccatgatcgc cgagctggga
      181 cgctaccggc atcaggtctt catcgagaag ctgggctggg acgtggtctc cacctccagg
      241 gtccgcgacc aggaattcga ccagttcgac catccgcaaa cccgctacat cgtcgccatg
      301 agccgccagg gcatctgcgg ttgcgcccgc ctgctgccga cgaccgacgc ctacctgctc
      361 aaggacgtct tcgcctacct gtgcagcgaa accccgccga gcgatccgtc ggtctgggag
      421 ctttcgcgct acgccgccag cgcggcggac gatccgcagc tggcgatgaa gatattctgg
      481 tccagcctgc aatgcgcctg gtacctgggc gccagttcgg tggtggcggt gaccaccacg
      541 gccatggagc gctatttcgt tcgcaacggc gtgatcctcc agcgcctcgg cccgccgcag
      601 aaggtcaagg gcgagacgct ggtcgcgatc agcttcccgg cctaccagga gcgcggcctg
      661 gagatgctgc tgcgctacca cccggaatgg ctgcagggcg taccgctgtc gatggcggtg
      721 acctccgcag cgaacgacga aaattacgcc cttgcagcgt aataaggtag agacctacta
      781 gtagcggccg ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      841 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      901 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      961 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1021 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1081 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1141 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1201 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1261 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1321 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1381 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1441 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1501 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1561 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1621 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1681 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1741 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1801 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1861 aatgataccg cgcgacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1921 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1981 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2041 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2101 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     2161 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     2221 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     2281 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     2341 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     2401 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2461 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2521 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2581 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2641 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2701 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga ca
//
