LOCUS       sequence-245426-        2155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly DE part for L3S3P11 (173x-strength 
            synthetic terminator, modified to remove BsaI cut site; see DOI: 
            10.1038/nmeth.2515)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2155)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: T15m
FEATURES             Location/Qualifiers
     source          1..2155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..23
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(27..70)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    73..94
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    171..191
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(197..214)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(368..956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(448..467)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1127..1918,1919..1987))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1750..1769
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1988..2092)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
       61 ggatgatttc tggaattcgc ggccgcttct agagactagt gggtctcaag gtccaattat
      121 tgaacaccct tcggggtgtt tttttgtttc tggtctgccg cttagagacc tactagtagc
      181 ggccgctgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
      241 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      301 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      361 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      421 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      481 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      541 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      601 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      661 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      721 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      781 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      841 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      901 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      961 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     1021 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     1081 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     1141 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     1201 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     1261 taccgcgcga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     1321 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     1381 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     1441 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     1501 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     1561 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     1621 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     1681 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     1741 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     1801 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     1861 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     1921 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     1981 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     2041 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     2101 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta accta
//
