LOCUS       sequence-24545-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Douglas PM, Treusch S, Ren HY, Halfmann R, Duennwald ML, Lindquist 
            S, Cyr DM
  TITLE     Chaperone-dependent amyloid assembly protects cells from prion 
            toxicity.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 May 20. 105(20):7206-11.
  PUBMED    18480252
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 May 20. 105(20):7206-11."
COMMENT     Sequence Label: pRS414 GPD-Sis1
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(18..207)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     219..237
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     255..271
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     256..272
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cggcggcgca attaagcctt cgagcgtccc aaaaccttct caagcaaggt tttcagtata
       61 atgttacatg cgtacacgcg tctgtacaga aaaaaaagaa aaatttgaaa tataaataac
      121 gttcttaata ctaacataac tataaaaaaa taaataggga cctagacttc aggttgtcta
      181 actccttcct tttcggttag agcggatgtg gggggagggc gtgaatgtaa gcgtgacata
      241 actaattaca tgactcgagg tcgacggtat cgataagctt gatatcgaat tcctgcagcc
      301 cgggggatcc ttaaaaattt tcatctatag cacgtttttg agcgtcgttt agtgatattg
      361 gatagtccac tttatatttt acaatcaaat tacctctctg agatgggttc tttggagttg
      421 gcataccttg accaggataa gtagaagttt gtgagggttg gacaggctgt actctcgaca
      481 aaggtaaggt tctgccatca attgtttgga tagtttttga aaaacctaac aatgattcct
      541 tgaaagatag tggcagagtg taaattaggt catcaccgtc tcttttaaag tttggatggc
      601 tcttttcctg gatgacaaac tgcaaagtct ttctacggcc cgttttgtgg aatgtaancc
      661 cnnggcntct ngtttttntt nggcnccccc cccccccccc gttttantga nggcatttgt
      721 gtctttncna ggcccagggg gcccttctcc atttaatgct ctttnccaca acaactcana
      781 aac
//
