LOCUS       sequence-24546-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Douglas PM, Treusch S, Ren HY, Halfmann R, Duennwald ML, Lindquist 
            S, Cyr DM
  TITLE     Chaperone-dependent amyloid assembly protects cells from prion 
            toxicity.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 May 20. 105(20):7206-11.
  PUBMED    18480252
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 May 20. 105(20):7206-11."
COMMENT     Sequence Label: pRS414 GPD-Sis1deltaG/F
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(19..208)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     220..238
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     256..272
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     257..273
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cggcggcgca attaagccct tcgagcgtcc caaaaccttc tcaagcaagg ttttcagtat
       61 aatgttacat gcgtacacgc gtctgtacag aaaaaaaaga aaaatttgaa atataaataa
      121 cgttcttaat actaacataa ctataaaaaa ataaataggg acctagactt caggttgtct
      181 aactccttcc ttttcggtta gagcggatgt ggggggaggg cgtgaatgta agcgtgacat
      241 aactaattac atgactcgag gtcgacggta tcgataagct tgatatcgaa ttcctgcagc
      301 ccgggggatc cttaaaaatt ttcatctata gcacgttttt gagcgtcgtt tagtgatatt
      361 ggatagtcca ctttatattt tacaatcaaa ttacctctct gagatgggtt ctttggagtt
      421 ggcatacctt gaccaggata agtagaagtt tgtgagggtt ggacaggctg tactctcgac
      481 aaaggtaagg ttctgccatc aattgtttgg atagtttttg aaaaacctaa caatgattcc
      541 ttgaaagata gtggcagagt gtaaattagg tcatcaccgt ctcttttaaa gtttggatgg
      601 ctcttttcct ggatgacaaa ctgcaaagtc tttctacgcc gcgttgnaga attgngacca
      661 ccctggtctg gngtgttttt nggncccnct nccncccggn tttnaattga ngncaattgt
      721 gtctttcaga gncccntgtg ggcccttctc cattttaatg actctttncc acaacaacct
      781 ccangcacgg gaaaact
//
