LOCUS       sequence-245478-       10508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid expresses Kir2.1 along with fluorescent marker RFP 
            under the control of CMV promoter. The vector also supports the 
            construction of adenovirus using ADeasy system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Wu Y, Ma L, Duyck K, Long CC, Moran A, Scheerer H, Blanck J, Peak A,
            Box A, Perera A, Yu CR
  TITLE     A Population of Navigator Neurons Is Essential for Olfactory Map 
            Formation during the Critical Period.
  JOURNAL   Neuron. 2018 Dec 5;100(5):1066-1082.e6. doi: 
            10.1016/j.neuron.2018.09.051. Epub 2018 Oct 25.
  PUBMED    30482691
REFERENCE   2  (bases 1 to 10508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2018.09.051"; journalName: "Neuron"; date: 
            "2018-12-5- 5"; volume: "100"; issue: "5"; pages: "1066-1082.e6"
COMMENT     Sequence Label: CMV-Kir2.1-pA-CMV-mRFP-pA(AV39)
FEATURES             Location/Qualifiers
     source          1..10508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     enhancer        3362..3665
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3666..3869
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3941..3950
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    5383..5504
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        5590..5893
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5894..6097
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             6140..6817
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             6140..6817
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(6270..6288)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6538..6557
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6732..6755
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    6965..7086
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_signal     7229..7379
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     repeat_region   7467..7569
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     rep_origin      7607..7987
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7694..7713)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7904..7925
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8035..8829)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(8035..8829)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8146..8165)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8756..8775
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      9661..10249
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10150..10169
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10403..10420
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg taggttttag ggcggagtaa
      121 cttgtatgtg ttgggaattg tagttttctt aaaatgggaa gtgacgtaac gtgggaaaac
      181 ggaagtgacg atttgaggaa gttgtgggtt ttttggcttt cgtttctggg cgtaggttcg
      241 cgtgcggttt tctgggtgtt ttttgtggac tttaaccgtt acgtcatttt ttagtcctat
      301 atatactcgc tctgcacttg gccctttttt acactgtgac tgattgagct ggtgccgtgt
      361 cgagtggtgt ttttttaata ggttttcttt tttactggta aggctgactg ttatggctgc
      421 cgctgtggaa gcgctgtatg ttgttctgga gcgggagggt gctattttgc ctaggcagga
      481 gggtttttca ggtgtttatg tgtttttctc tcctattaat tttgttatac ctcctatggg
      541 ggctgtaatg ttgtctctac gcctgcgggt atgtattccc ccgggctatt tcggtcgctt
      601 tttagcactg accgatgtga atcaacctga tgtgtttacc gagtcttaca ttatgactcc
      661 ggacatgacc gaggagctgt cggtggtgct ttttaatcac ggtgaccagt ttttttacgg
      721 tcacgccggc atggccgtag tccgtcttat gcttataagg gttgtttttc ctgttgtaag
      781 acaggcttct aatgtttaaa tgtttttttg ttattttatt ttgtgtttat gcagaaaccc
      841 gcagacatgt ttgagagaaa aatggtgtct ttttctgtgg tggttccgga gcttacctgc
      901 ctttatctgc atgagcatga ctacgatgtg ctttcttttt tgcgcgaggc tttgcctgat
      961 tttttgagca gcaccttgca ttttatatcg ccgcccatgc aacaagctat tgaattcgtt
     1021 taaactccct ctcaagtctg tatacgggga cacggacagc cttttcgtca ccgagcgtgg
     1081 acaccggctc atggaaacca gaggtaagaa acgcatcaaa aagcatgggg gaaacctggt
     1141 ttttgacccc gaacggccag agctcacctg gctcgtggaa tgcgagaccg tctgcggggc
     1201 ctgcggcgcg gatgcctact ccccggaatc ggtatttctc gcgcccaagc tctacgccct
     1261 caaaagtctg cactgcccct cgtgcggcgc ctcctccaag ggcaagctgc gcgccaaggg
     1321 ccacgccgcg gaggggctgg actatgacac catggtcaaa tgctacctgg ccgacgcgca
     1381 gggcgaagac cggcagcgct tcagcaccag caggaccagc ctcaagcgca ccctggccag
     1441 cgcgcagccc ggagcgcacc ccttcaccgt gacccagact acgctgacga ggaccctgcg
     1501 cccgtggaaa gacatgaccc tggcccgtct ggacgagcac cgactactgc cgtacagcga
     1561 aagccgcccc aacccgcgaa acgaggagat atgctggatc gagatgccgt agagcaggtg
     1621 accgagctgt gggaccgcct ggaactgctt ggtcaaacgc tcaaaagcat gcctacggcg
     1681 gacggtctca aaccgttgaa aaactttgct tccttgcaag aactgctatc gctgggcggc
     1741 gagcgccttc tggcggattt ggtcagggaa aacatgcgag tcagggacat gcttaacgaa
     1801 gtggcccccc tgctcaggga tgacggcagc tgcagctctc ttaactacca gttgcagccg
     1861 gtaataggtg tgatttacgg gcccaccggc tgcggtaagt cgcagctgct caggaacctg
     1921 ctttcttccc agctgatctc ccctaccccg gaaaccgttt tcttcatcgc cccgcaggta
     1981 gacatgatcc ccccatctga actcaaagcg tgggaaatgc aaatctgtga gggtaactac
     2041 gcccctgggc cggatggaac cattataccg cagtctggca ccctccgccc gcgctttgta
     2101 aaaatggcct atgacgatct catcctggaa cacaactatg acgttagtga tcccagaaat
     2161 atcttcgccc aggccgccgc ccgtgggccc attgccatca ttatggacga atgcatggaa
     2221 aatcttggag gtcacaaggg cgtctccaag ttcttccacg catttccttc taagctacat
     2281 gacaaatttc ccaagtgcac cggatacact gtgctggtgg ttctgcacaa catgaatccc
     2341 cggagggata tggctgggaa catagccaac ctaaaaatac agtccaagat gcatctcata
     2401 tccccacgta tgcacccatc ccagcttaac cgctttgtaa acacttacac caagggcctg
     2461 cccctggcaa tcagcttgct actgaaagac atttttaggc accacgccca gcgctcctgc
     2521 tacgactgga tcatctacaa caccaccccg cagcatgaag ctctgcagtg gtgctacctc
     2581 caccccagag acgggcttat gcccatgtat ctgaacatcc agagtcacct ttaccacgtc
     2641 ctggaaaaaa tacacaggac cctcaacgac cgagaccgct ggtcccgggc ctaccgcgcg
     2701 cgcaaaaccc ctaaataaag acagcaagac acttgcttga tccaaatcca aacagagtct
     2761 ggttttttat ttatgtttta aaccgcattg ggaggggagg aagccttcag ggcagaaacc
     2821 tgctggcgca gatccaacag ctgctgagaa acgacattaa gttcccgggt caaagaatcc
     2881 aattgtgcca aaagagccgt caacttgtca tcgcgggcgg atgaacggga agctgcactg
     2941 cttgcaagcg ggctcaggaa agcaaagtca gtcacaatcc cgcgggcggt ggctgcagcg
     3001 gctgaagcgg cggcggaggc tgcagtctcc aacggcgttc cagacacggt ctcgtaggtc
     3061 aaggtagtag agtttgcggg caggacgggg cgaccatcaa tgctggagcc catcacattc
     3121 tgacgcaccc cggcccatgg gggcatgcgc gttgtcaaat atgagctcac aatgcttcca
     3181 tcaaacgagt tggtgctcat ggcggcggcg gctgctgcaa aacagataca aaactacata
     3241 agacccccac cttatatatt ctttcccacc cttaaccacg cccagatcct ctagcagtga
     3301 taaacgtcta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     3361 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     3421 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     3481 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     3541 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     3601 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     3661 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     3721 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     3781 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     3841 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagagat
     3901 ctggtaccgt cgacgcggcc gcgaattcgc ccttggccgc gccaccatgg gcagtgtgag
     3961 aaccaaccgc tacagcatcg tctcttcgga ggaagatggc atgaagctgg ccactatggc
     4021 agttgccaat ggctttggga atggcaagag taaagtccat acccgacaac agtgcaggag
     4081 ccgctttgtg aagaaagatg gtcattgcaa tgttcagttt atcaacgtgg gtgagaaggg
     4141 acagaggtac ctggcagaca tctttactac ctgtgtcgac atccgctgga ggtggatgct
     4201 ggttatcttc tgtcttgcct tcgtgctctc ctggctgttc tttggctgtg tgttttggtt
     4261 gatagccctg ctccatgggg atctagatac ttctaaagtg agcaaagcat gcgtgtcaga
     4321 ggtcaacagc ttcacggctg ccttcctctt ctccatcgag acccagacaa ccattggcta
     4381 tggtttcagg tgtgtgacag acgagtgccc aattgctgtc ttcatggtgg tattccagtc
     4441 aatcgtaggc tgcatcattg acgccttcat cattggtgca gtcatggcga agatggcaaa
     4501 gccaaagaag agaaatgaga ctcttgtctt cagtcacaat gctgtgattg ccatgaggga
     4561 tggcaaactc tgcttgatgt ggagagtggg taaccttcga aagagccacc ttgtggaagc
     4621 tcatgtccgg gcacagcttc tcaaatctag gatcacttca gaaggggagt atatcccttt
     4681 ggaccagata gacatcaatg ttggttttga tagtggaatt gaccgcatat ttctagtgtc
     4741 ccccatcact atcgttcacg aaatagatga agacagccct ttatatgact tgagtaagca
     4801 ggacattgac aatgcagact ttgaaattgt tgtcatactg gaaggcatgg tggaggcgac
     4861 tgccatgaca actcaatgcc ggagttcgta tctggccaat gaaattctct ggggtcaccg
     4921 ctatgagcca gtgctctttg aagagaaaca ctactataaa gtagactatt caagattcca
     4981 taagacttat gaagtaccta acacccccct ttgtagtgcc agagacttag cagagaagaa
     5041 atacatcctt tcaaatgcaa attcattttg ctatgaaaat gaagttgccc taacaagcaa
     5101 agaggaagag gaggatagtg agaacggagt cccagagagc acaagcacag actcacctcc
     5161 tggcatagat ctccacaacc aggcaagcgt acctctagag cccaggccct taaggcgaga
     5221 atcggagata tgaaagcttc tagataagat atccgatcca ccggatctag ataactgatc
     5281 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     5341 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     5401 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     5461 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgg aggtttatcg
     5521 acgatctgct agtgattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     5581 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     5641 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     5701 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     5761 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     5821 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     5881 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     5941 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     6001 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     6061 taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg
     6121 ctagcgctac cgggatccga tggcctcctc cgaggacgtc atcaaggagt tcatgcgctt
     6181 caaggtgcgc atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     6241 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct
     6301 gcccttcgcc tgggacatcc tgtcccctca gttccagtac ggctccaagg cctacgtgaa
     6361 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     6421 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     6481 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     6541 cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcggatgt accccgagga
     6601 cggcgccctg aagggcgaga tcaagatgag gctgaagctg aaggacggcg gccactacga
     6661 cgccgaggtc aagaccacct acatggccaa gaagcccgtg cagctgcccg gcgcctacaa
     6721 gaccgacatc aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     6781 cgagcgcgcc gagggccgcc actccaccgg cgcctaagaa ttggccgcga ctctagatca
     6841 taatcagcca taccacaaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     6901 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     6961 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7021 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7081 atcttaacgc gatcaagcta gcttgctaga ctcgactgac tataataata aaacgccaac
     7141 tttgacccgg aacgcggaaa acacctgaga aaaacacctg ggcgagtctc cacgtaaacg
     7201 gtcaaagtcc ccgcggccct agacaaatat tacgcgctat gagtaacaca aaattattca
     7261 gatttcactt cctcttattc agttttcccg cgaaaatggc caaatcttac tcggttacgc
     7321 ccaaatttac tacaacatcc gcctaaaacc gcgcgaaaat tgtcacttcc tgtgtacacc
     7381 ggcgcacacc aaaaacgtca cttttgccac atccgtcgct tacatgtgtt ccgccacact
     7441 tgcaacatca cacttccgcc acactactac gtcacccgcc ccgttcccac gccccgcgcc
     7501 acgtcacaaa ctccaccccc tcattatcat attggcttca atccaaaata aggtatatta
     7561 ttgatgatgt taattaagaa ttaattcgat cctgaatggc gaatggacgc gccctgtagc
     7621 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     7681 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     7741 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     7801 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     7861 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     7921 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     7981 atttcggcct attggttaaa aaatgagctg atttaacaaa aattttaaca aaattcagaa
     8041 gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta
     8101 aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc
     8161 caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga
     8221 aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag
     8281 atcctcgccg tcgggcatgc tcgccttgag cctggcgaac agttcggctg gcgcgagccc
     8341 ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc
     8401 tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg
     8461 cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga
     8521 caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac
     8581 aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc
     8641 ctcgtcttgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg
     8701 cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca
     8761 gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg
     8821 ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gagcttgatc ccctgcgcca
     8881 tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc
     8941 agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag
     9001 ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct
     9061 tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct gcggactggc
     9121 tttctacgtg aaaaggatct aggtgaagat cctttttgat aatctcatgg ctgcagcaat
     9181 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     9241 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     9301 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     9361 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     9421 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     9481 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     9541 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     9601 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     9661 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9721 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9781 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     9841 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9901 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9961 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    10021 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    10081 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    10141 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    10201 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    10261 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    10321 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    10381 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
    10441 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatgga tccatgcatg
    10501 ttaattaa
//
