LOCUS       sequence-24553-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Yasuda R, Harvey CD, Zhong H, Sobczyk A, van Aelst L, Svoboda K
  TITLE     Supersensitive Ras activation in dendrites and spines revealed by 
            two-photon fluorescence lifetime imaging.
  JOURNAL   Nat Neurosci. 2006 Feb . 9(2):283-91.
  PUBMED    16429133
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2006 Feb . 9(2):283-91."
COMMENT     Sequence Label: mRFP-RBD(R59A)-mRFP
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..41
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          157..289
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     272..291
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        334..352
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      372..381
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(509..527)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cnnngggnat tagcagagct cgtttagtga accgtcagat cactagaagc tttattgcgg
       61 tagtttatca cagnttaaat tgctaacgca gtcagtgctt ctgacacaac agtctcgaac
      121 ttaagctgca gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag
      181 gtttaaggag accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga
      241 taggcaccta ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc
      301 cagttcaatt acagctctta aggctagagt acttaatacg actcactata ggctagcctc
      361 gagaattcgt cgccaccatg gcctcctccg aggacgtcat caaggagttc atgcgcttca
      421 aggtgcgcat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gaggggcgag
      481 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
      541 cccttcgcct gggacatcct gtcccctcag ttccagtacg gctccaaggc ctacgtgaag
      601 caccccgccg acatccccga ctacttgnnc gnnnnnntnc cnganntttg ngggnnnnac
      661 ncgtgatgaa cttcnaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
      721 acggcgagtt catctacaag tganctgcgc ggcacnacca acttcccctc cgacggcccg
      781 tatgcaaaaa agacatgggc tggaagcctc cccgacgaat tacccaagac gnncctgang
      841 gnaannaaan nagt
//
