LOCUS       sequence-245530-       14998 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for the inducible expression of GFP1–10 fragment that 
            can be used to detect a protein tagged with GFP11.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14998)
  AUTHORS   Liu TY
  TITLE     Using Tripartite Split-sfGFP for the Study of Membrane 
            Protein-Protein Interactions.
  JOURNAL   Methods Mol Biol. 2021;2200:323-336. doi: 
            10.1007/978-1-0716-0880-7_15.
  PUBMED    33175385
REFERENCE   2  (bases 1 to 14998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2021"; volume: "2200:323-336. doi"; pages: " 
            10.1007/978-1-0716-0880-7_15"
COMMENT     Sequence Label: UBQ10:sXVE:GFP1–10
FEATURES             Location/Qualifiers
     source          1..14998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(28..831)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(139..158)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     749..768
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(881..1060)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        4322..4368
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      5098..5350
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5518..5542
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6007..6657
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     primer_bind     complement(6845..6862)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7096..7115)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     oriT            7338..7447
                     /note="incP origin of transfer"
     CDS             7480..7851
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     rep_origin      7978..8689
                     /label=oriV
                     /note="incP origin of replication"
     mobile_element  9312..10079
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             10271..11065
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             11364..12512
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    14108..14132
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(14379..14400)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14591..14610
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14735..14757
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14749..14766
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14750..14766
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(14779..14979)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
ORIGIN
        1 atccgtcgaa gctagcttgg gtcccgctca gaagaactcg tcaagaaggc gatagaaggc
       61 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
      121 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
      181 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
      241 cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt
      301 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
      361 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
      421 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
      481 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
      541 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
      601 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc
      661 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
      721 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
      781 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc caagctccca tgggccctcg
      841 actagagtcg agatctggat tgagagtgaa tatgagactc taattggata ccgaggggaa
      901 tttatggaac gtcagtggag catttttgac aagaaatatt tgctagctga tagtgacctt
      961 aggcgacttt tgaacgcgca ataatggttt ctgacgtatg tgcttagctc attaaactcc
     1021 agaaacccgc ggctgagtgg ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa
     1081 cggcttgtcc cgcgtcatcg gcgggggtca taacgtgact cccttaattc tccgctcatg
     1141 atcttgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc
     1201 agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc
     1261 ataaaaccgc ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct
     1321 ttgcgcttgc gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc
     1381 accgtttctg cggactggct ttctacgtgt tccgcttcct ttagcagccc ttgcgccctg
     1441 agtgcttgcg gcagcgtgaa gcttcgacga gtcagtaata aacggcgtca aagtggttgc
     1501 agccggcaca cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa
     1561 aaataaggca attagccaaa aacaactttg cgtgtaaaca acgctcaata cacgtgtcat
     1621 tttattatta gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt
     1681 ttcttcttct tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt
     1741 tcaatttctc aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat
     1801 ttctgtgttc cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt
     1861 atatgttctt tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat
     1921 cgttagatat catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa
     1981 taatttgagt tttgtcgaat aattactctt cgatttgtga tttctatcta gttctggtgt
     2041 tagtttctag tttgtgcgat cgaatttgtc gattaatctg agtttttctg attaacagac
     2101 tagcccgcgg aaaatgaagg ctcttactgc tagacaacaa gaagtttttg atcttattag
     2161 agatcatatt tctcaaactg gaatgcctcc tactagagct gaaattgctc aaagacttgg
     2221 atttaggtct cctaatgctg ctgaagaaca tcttaaggct cttgctagaa agggagttat
     2281 tgaaattgtt tctggagctt caagaggaat tagacttctt caagaagaag aagaaggact
     2341 tcctcttgtt ggaagagttg ctgctggaga accttcttct gctcctccta ctgatgtttc
     2401 tcttggagat gaacttcatc ttgatggaga agatgttgct atggctcatg ctgatgctct
     2461 tgatgatttt gatcttgata tgcttggaga tggagattct cctggacctg gatttactcc
     2521 tcatgattct gctccttatg gagcacttga tatggctgat tttgaatttg aacaaatgtt
     2581 tactgatgct cttggaattg atgaatatgg aggagatcct tctgctggag atatgagagc
     2641 tgctaatctt tggccttctc ctcttatgat taagaggtct aagaagaact ctcttgctct
     2701 ttctcttact gctgaccaaa tggtttctgc tcttcttgat gctgaacctc ctattcttta
     2761 ttctgaatat gatcctacta gacctttttc tgaggcttct atgatgggac ttcttactaa
     2821 tcttgctgat agagaacttg ttcatatgat taattgggct aagagagttc ctggatttgt
     2881 tgatcttact cttcatgacc aagttcatct tcttgaatgt gcttggcttg aaattcttat
     2941 gattggactt gtttggaggt ctatggaaca tcctgttaaa cttctttttg ctcctaatct
     3001 tcttcttgat agaaatcaag gaaagtgtgt tgaaggaatg gttgaaattt ttgatatgct
     3061 tcttgctact tcttcaagat ttagaatgat gaatcttcaa ggagaagaat ttgtttgtct
     3121 taagtctatt attcttctta attctggagt ttatactttt ctttcttcta ctcttaagtc
     3181 tcttgaagaa aaggatcata ttcatagagt tcttgataag attactgata ctcttattca
     3241 tcttatggct aaggctggac ttactcttca acaacaacat caaagacttg ctcaacttct
     3301 tcttattctt tctcatatta gacatatgtc taataaggga atggaacatc tttattctat
     3361 gaagtgtaag aatgttgttc ctctttatga tcttcttctt gaaatgcttg atgctcatag
     3421 acttcatgct cctacttcaa gaggaggagc ttctgttgaa gaaactgacc aatctcatct
     3481 tgctactgct ggatctactt cttctcattc tcttcaaaag tattatatta ctggagaagc
     3541 tgaaggattt cctgctactg tttgaagatc ttgatcaagc tccctggcca attcccagag
     3601 atgttagctg aaatcatcac taatcagata ccaaaatatt caaatggaaa tatcaaatag
     3661 cttctgtttc atcaaaaatg actcgaccta actgagtaag catgcttgtt cgagtattat
     3721 ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt
     3781 tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga
     3841 tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt
     3901 gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa
     3961 tcgtggcctc taatgaccga agttaatatg aggagtaaaa catcccaaac gcggccgcaa
     4021 ccggtcttgc atccagctgg gctgcaggtc gaggctaaaa aactaatcgc attatcatcc
     4081 cctcgacgta ctgtacatat aaccactggt tttatataca gcagtactgt acatataacc
     4141 actggtttta tatacagcag tcgacgtact gtacatataa ccactggttt tatatacagc
     4201 agtactgtac atataaccac tggttttata tacagcagtc gaggtaagat tagatatgga
     4261 tatgtatatg gatatgtata tggtggtaat gccatgtaat atgctcgact ctaggatctt
     4321 cgcaagaccc ttcctctata taaggaagtt catttcattt ggagaggaca cgctgaagct
     4381 agtcgactct agccaaatct agagttaacc gggctcaggc ctggcgcgcc actagtatgt
     4441 ccaaaggaga agaacttttc actggagttg tcccaattct tgtcgaatta gatggtgatg
     4501 ttaatgggca caaattttca gtccgtggag agggtgaagg tgatgcaaca atcggaaaac
     4561 ttacacttaa atttatttgc actactggaa aactacctgt tccctggtca acacttgtca
     4621 ctacccttac ctatggtgtt caatgctttt caagataccc agatcacatg aagcggcacg
     4681 acttcttcaa gagcgccatg cctgagggat acgtgcagga gaggaccatc tcgttcaagg
     4741 acgacgggaa atacaagaca cgtgctgttg tcaagtttga gggagacacc ctcgtcaaca
     4801 ggatcgagct taagggaaca gatttcaagg aggacggaaa catcctcggc cacaagttgg
     4861 aatacaactt taactcccac aacgtataca tcacggccga caagcaaaag aacggcatca
     4921 aagccaactt cacagtacgc cacaacgttg aagacggctc tgtgcaactc gctgatcatt
     4981 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
     5041 ccacacaaac agtcctttcc aaagatccca acgaaaagta agagctcgaa tttccccgat
     5101 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
     5161 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
     5221 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
     5281 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
     5341 ttactagatc ggggaattca gatcggctga gtggctcctt caacgttgcg gttctgtcag
     5401 ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta
     5461 attctccgct catgatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga
     5521 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt
     5581 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
     5641 ttccccagat ctggcgccgg ccagcgagac gagcaagatt ggccgccgcc cgaaacgatc
     5701 cgacagcgcg cccagcacag gtgcgcaggc aaattgcacc aacgcataca gcgccagcag
     5761 aatgccatag tgggcggtga cgtcgttcga gtgaaccaga tcgcgcagga ggcccggcag
     5821 caccggcata atcaggccga tgccgacagc gtcgagcgcg acagtgctca gaattacgat
     5881 caggggtatg ttgggtttca cgtctggcct ccggaccagc ctccgctggt ccgattgaac
     5941 gcgcggattc tttatcactg ataagttggt ggacatatta tgtttatcag tgataaagtg
     6001 tcaagcatga caaagttgca gccgaataca gtgatccgtg ccgccctgga cctgttgaac
     6061 gaggtcggcg tagacggtct gacgacacgc aaactggcgg aacggttggg ggttcagcag
     6121 ccggcgcttt actggcactt caggaacaag cgggcgctgc tcgacgcact ggccgaagcc
     6181 atgctggcgg agaatcatac gcattcggtg ccgagagccg acgacgactg gcgctcattt
     6241 ctgatcggga atgcccgcag cttcaggcag gcgctgctcg cctaccgcga tggcgcgcgc
     6301 atccatgccg gcacgcgacc gggcgcaccg cagatggaaa cggccgacgc gcagcttcgc
     6361 ttcctctgcg aggcgggttt ttcggccggg gacgccgtca atgcgctgat gacaatcagc
     6421 tacttcactg ttggggccgt gcttgaggag caggccggcg acagcgatgc cggcgagcgc
     6481 ggcggcaccg ttgaacaggc tccgctctcg ccgctgttgc gggccgcgat agacgccttc
     6541 gacgaagccg gtccggacgc agcgttcgag cagggactcg cggtgattgt cgatggattg
     6601 gcgaaaagga ggctcgttgt caggaacgtt gaaggaccga gaaagggtga cgattgatca
     6661 ggaccgctgc cggagcgcaa cccactcact acagcagagc catgtagaca acatcccctc
     6721 cccctttcca ccgcgtcaga cgcccgtagc agcccgctac gggctttttc atgccctgcc
     6781 ctagcgtcca agcctcacgg ccgcgctcgg cctctctggc ggccttctgg cgctcttccg
     6841 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     6901 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     6961 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7021 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7081 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7141 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7201 cgcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca
     7261 tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt
     7321 atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc
     7381 ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc
     7441 tggccggcta ccgccggcgt aacagatgag ggcaagcgga tggctgatga aaccaagcca
     7501 accaggaagg gcagcccacc tatcaaggtg tactgccttc cagacgaacg aagagcgatt
     7561 gaggaaaagg cggcggcggc cggcatgagc ctgtcggcct acctgctggc cgtcggccag
     7621 ggctacaaaa tcacgggcgt cgtggactat gagcacgtcc gcgagctggc ccgcatcaat
     7681 ggcgacctgg gccgcctggg cggcctgctg aaactctggc tcaccgacga cccgcgcacg
     7741 gcgcggttcg gtgatgccac gatcctcgcc ctgctggcga agatcgaaga gaagcaggac
     7801 gagcttggca aggtcatgat gggcgtggtc cgcccgaggg cagagccatg acttttttag
     7861 ccgctaaaac ggccgggggg tgcgcgtgat tgccaagcac gtccccatgc gctccatcaa
     7921 gaagagcgac ttcgcggagc tggtgaagta catcaccgac gagcaaggca agaccgagcg
     7981 cctttccgac gctcaccggg ctggttgccc tcgccgctgg gctggcggcc gtctatggcc
     8041 ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag acaccgcggc cgccggcgtt
     8101 gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac gttgacactt
     8161 gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga tttcggccgg
     8221 cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc
     8281 cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac ttgaggggcg
     8341 cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt gctgacagat
     8401 gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc agcatttgca
     8461 agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct tttaaaccaa
     8521 tatttataaa ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga
     8581 aggggggtgc ccccccttct cgaaccctcc cggcccgcta acgcgggcct cccatccccc
     8641 caggggctgc gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc gctggcagtc
     8701 cttgccattg ccgggatcgg ggcagtaacg ggatgggcga tcagcccgag cgcgacgccc
     8761 ggaagcattg acgtgccgca ggtgctggca tcgacattca gcgaccaggt gccgggcagt
     8821 gagggcggcg gcctgggtgg cggcctgccc ttcacttcgg ccgtcggggc attcacggac
     8881 ttcatggcgg ggccggcaat ttttaccttg ggcattcttg gcatagtggt cgcgggtgcc
     8941 gtgctcgtgt tcgggggtgc gataaaccca gcgaaccatt tgaggtgata ggtaagatta
     9001 taccgaggta tgaaaacgag aattggacct ttacagaatt actctatgaa gcgccatatt
     9061 taaaaagcta ccaagacgaa gaggatgaag aggatgagga ggcagattgc cttgaatata
     9121 ttgacaatac tgataagata atatatcttt tatatagaag atatcgccgt atgtaaggat
     9181 ttcagggggc aaggcatagg cagcgcgctt atcaatatat ctatagaatg ggcaaagcat
     9241 aaaaacttgc atggactaat gcttgaaacc caggacaata accttatagc ttgtaaattc
     9301 tatcataatt gggtaatgac tccaacttat tgatagtgtt ttatgttcag ataatgcccg
     9361 atgactttgt catgcagctc caccgatttt gagaacgaca gcgacttccg tcccagccgt
     9421 gccaggtgct gcctcagatt caggttatgc cgctcaattc gctgcgtata tcgcttgctg
     9481 attacgtgca gctttccctt caggcgggat tcatacagcg gccagccatc cgtcatccat
     9541 atcaccacgt caaagggtga cagcaggctc ataagacgcc ccagcgtcgc catagtgcgt
     9601 tcaccgaata cgtgcgcaac aaccgtcttc cggagactgt catacgcgta aaacagccag
     9661 cgctggcgcg atttagcccc gacatagccc cactgttcgt ccatttccgc gcagacgatg
     9721 acgtcactgc ccggctgtat gcgcgaggtt accgactgcg gcctgagttt tttaagtgac
     9781 gtaaaatcgt gttgaggcca acgcccataa tgcgggctgt tgcccggcat ccaacgccat
     9841 tcatggccat atcaatgatt ttctggtgcg taccgggttg agaagcggtg taagtgaact
     9901 gcagttgcca tgttttacgg cagtgagagc agagatagcg ctgatgtccg gcggtgcttt
     9961 tgccgttacg caccaccccg tcagtagctg aacaggaggg acagctgata gaaacagaag
    10021 ccactggagc acctcaaaaa caccatcata cactaaatca gtaagttggc agcatcaccc
    10081 ataattgtgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac tttgaaaaca
    10141 actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac agtgaattgg
    10201 agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga aaagaggaag
    10261 gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga tcgaaaaata
    10321 ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc tggtgggaga
    10381 aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca cctatgatgt
    10441 ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc caaaggtcct
    10501 gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg atggcgtcct
    10561 ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc tgtatgcgga
    10621 gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga atagcttaga
    10681 cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg tggattgcga
    10741 aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt ttttaaagac
    10801 ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca gcaacatctt
    10861 tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca gggcggacaa
    10921 gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg aagaacagta
    10981 tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatta
    11041 tattttactg gatgaattgt tttagtacct agatgtggcg caacgatgcc ggcgacaagc
    11101 aggagcgcac cgacttcttc cgcatcaagt gttttggctc tcaggccgag gcccacggca
    11161 agtatttggg caaggggtcg ctggtattcg tgcagggcaa gattcggaat accaagtacg
    11221 agaaggacgg ccagacggtc tacgggaccg acttcattgc cgataaggtg gattatctgg
    11281 acaccaaggc accaggcggg tcaaatcagg aataagggca cattgccccg gcgtgagtcg
    11341 gggcaatccc gcaaggaggg tgaatgaatc ggacgtttga ccggaaggca tacaggcaag
    11401 aactgatcga cgcggggttt tccgccgagg atgccgaaac catcgcaagc cgcaccgtca
    11461 tgcgtgcgcc ccgcgaaacc ttccagtccg tcggctcgat ggtccagcaa gctacggcca
    11521 agatcgagcg cgacagcgtg caactggctc cccctgccct gcccgcgcca tcggccgccg
    11581 tggagcgttc gcgtcgtctc gaacaggagg cggcaggttt ggcgaagtcg atgaccatcg
    11641 acacgcgagg aactatgacg accaagaagc gaaaaaccgc cggcgaggac ctggcaaaac
    11701 aggtcagcga ggccaagcag gccgcgttgc tgaaacacac gaagcagcag atcaaggaaa
    11761 tgcagctttc cttgttcgat attgcgccgt ggccggacac gatgcgagcg atgccaaacg
    11821 acacggcccg ctctgccctg ttcaccacgc gcaacaagaa aatcccgcgc gaggcgctgc
    11881 aaaacaaggt cattttccac gtcaacaagg acgtgaagat cacctacacc ggcgtcgagc
    11941 tgcgggccga cgatgacgaa ctggtgtggc agcaggtgtt ggagtacgcg aagcgcaccc
    12001 ctatcggcga gccgatcacc ttcacgttct acgagctttg ccaggacctg ggctggtcga
    12061 tcaatggccg gtattacacg aaggccgagg aatgcctgtc gcgcctacag gcgacggcga
    12121 tgggcttcac gtccgaccgc gttgggcacc tggaatcggt gtcgctgctg caccgcttcc
    12181 gcgtcctgga ccgtggcaag aaaacgtccc gttgccaggt cctgatcgac gaggaaatcg
    12241 tcgtgctgtt tgctggcgac cactacacga aattcatatg ggagaagtac cgcaagctgt
    12301 cgccgacggc ccgacggatg ttcgactatt tcagctcgca ccgggagccg tacccgctca
    12361 agctggaaac cttccgcctc atgtgcggat cggattccac ccgcgtgaag aagtggcgcg
    12421 agcaggtcgg cgaagcctgc gaagagttgc gaggcagcgg cctggtggaa cacgcctggg
    12481 tcaatgatga cctggtgcat tgcaaacgct agggccttgt ggggtcagtt ccggctgggg
    12541 gttcagcagc cagcgcttta ctggcatttc aggaacaagc gggcactgct cgacgcactt
    12601 gcttcgctca gtatcgctcg ggacgcacgg cgcgctctac gaactgccga taaacagagg
    12661 attaaaattg acaattgtga ttaaggctca gattcgacgg cttggagcgg ccgacgtgca
    12721 ggatttccgc gagatccgat tgtcggccct gaagaaagct ccagagatgt tcgggtccgt
    12781 ttacgagcac gaggagaaaa agcccatgga ggcgttcgct gaacggttgc gagatgccgt
    12841 ggcattcggc gcctacatcg acggcgagat cattgggctg tcggtcttca aacaggagga
    12901 cggccccaag gacgctcaca aggcgcatct gtccggcgtt ttcgtggagc ccgaacagcg
    12961 aggccgaggg gtcgccggta tgctgctgcg ggcgttgccg gcgggtttat tgctcgtgat
    13021 gatcgtccga cagattccaa cgggaatctg gtggatgcgc atcttcatcc tcggcgcact
    13081 taatatttcg ctattctgga gcttgttgtt tatttcggtc taccgcctgc cgggcggggt
    13141 cgcggcgacg gtaggcgctg tgcagccgct gatggtcgtg ttcatctctg ccgctctgct
    13201 aggtagcccg atacgattga tggcggtcct gggggctatt tgcggaactg cgggcgtggc
    13261 gctgttggtg ttgacaccaa acgcagcgct agatcctgtc ggcgtcgcag cgggcctggc
    13321 gggggcggtt tccatggcgt tcggaaccgt gctgacccgc aagtggcaac ctcccgtgcc
    13381 tctgctcacc tttaccgcct ggcaactggc ggccggagga cttctgctcg ttccagtagc
    13441 tttagtgttt gatccgccaa tcccgatgcc tacaggaacc aatgttctcg gcctggcgtg
    13501 gctcggcctg atcggagcgg gtttaaccta cttcctttgg ttccggggga tctcgcgact
    13561 cgaacctaca gttgtttcct tactgggctt tctcagcccc agatctgggg tcgatcagcc
    13621 ggggatgcat caggccgaca gtcggaactt cgggtccccg acctgtacca ttcggtgagc
    13681 aatggatagg ggagttgata tcgtcaacgt tcacttctaa agaaatagcg ccactcagct
    13741 tcctcagcgg ctttatccag cgatttccta ttatgtcggc atagttctca agatcgacag
    13801 cctgtcacgg ttaagcgaga aatgaataag aaggctgata attcggatct ctgcgaggga
    13861 gatgatattt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
    13921 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
    13981 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
    14041 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
    14101 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca
    14161 cattgcggac gtttttaatg tactggggtg gtttttcttt tcaccagtga gacgggcaac
    14221 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt
    14281 tgccccagca ggcgaaaatc ctgtttgatg gtggttccga aatcggcaaa atcccttata
    14341 aatcaaaaga atagcccgag atagggttga gtgttgttcc agtttggaac aagagtccac
    14401 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
    14461 cactacgtga accatcaccc aaatcaagtt ttttggggtc gaggtgccgt aaagcactaa
    14521 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
    14581 cgagaaagga agggaagaaa gcgaaaggag cgggcgccat tcaggctgcg caactgttgg
    14641 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
    14701 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
    14761 gccagtgaat taattcccat cttgaaagaa atatagttta aatatttatt gataaaataa
    14821 caagtcaggt attatagtcc aagcaaaaac ataaatttat tgatgcaagt ttaaattcag
    14881 aaatatttca ataactgatt atatcagctg gtacattgcc gtagatgaaa gactgagtgc
    14941 gatattatgt gtaatacata aattgatgat atagctagct tagctcatcg ggggatcg
//
