LOCUS       sequence-245547-        2900 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target plasmid containing PSP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Strecker J, Ladha A, Gardner Z, Schmid-Burgk JL, Makarova KS, Koonin
            EV, Zhang F
  TITLE     RNA-guided DNA insertion with CRISPR-associated transposases.
  JOURNAL   Science. 2019 Jul 5;365(6448):48-53. doi: 10.1126/science.aax9181. 
            Epub 2019 Jun 6.
  PUBMED    31171706
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aax9181"; journalName: "Science"; date: "2019-07-5-
            5"; volume: "365"; issue: "6448"; pages: "48-53"
COMMENT     Sequence Label: pTarget_CAST
FEATURES             Location/Qualifiers
     source          1..2900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(416..433)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(550..1095)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(630..649)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(1712..1731)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(2162..2821)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2736..2755
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2822..2900,1..24))
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 ttggaacctc ttacgtgccg atcaacgtct cattttcgcc aaaagttggc ccagggcttc
       61 ccggtatcaa cagggacacc aggatttatt tattctgcga agtgatcttc cgtcacaggt
      121 atttattcgg cgcaaagtgc gtcgggtgat gctgccaact tactgattta gtgtatgatg
      181 gtgtttttga ggtgctccag tggcttctgt ttctatcagc tgtccctcct gttcagctac
      241 tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat cagcgctagc ggagtgtata
      301 ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt ggcaggagaa
      361 aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc gcttcctcgc
      421 tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct tacgaacggg
      481 gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga gggccgcggc
      541 aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct gacgctcaaa
      601 tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggcggctc
      661 cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc tgttatggcc
      721 gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc tccaagctgg
      781 actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt aactatcgtc
      841 ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact ggtaattgat
      901 ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag gacaagtttt
      961 ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc tcagagaacc
     1021 ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat tacgcgcaga
     1081 ccaaaacgat ctcaagaaga tcatcttatt aatatacgta gtatctacga tacgtagtat
     1141 ctacgatacg taggaggttg agaagtcatt taataaggcc actgttaaac gtagatatat
     1201 ctacgcgtag atatatctac gatacgtagc ggccgacgcg ctgggctacg tctttaatgg
     1261 gcagcgccca catacgcagc gatttccaac agcttgaggc ggaagcttta atgcggtagt
     1321 ttatcacagt taaattgcta acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc
     1381 atcgtcatcc tcggcaccgt caccctggat gctgtaggca taggcttggt tatgccggta
     1441 ctgccgggcc tcttgcggga tatcgtccat tccgacagca tcgccagtca ctatggcgtg
     1501 ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc
     1561 gaccgctttg gccgccgccc agtcctgctc gcttcgctac ttggagccac tatcgactac
     1621 gcgatcatgg cgaccacacc cgtcctgtgg atcctctacg ccggacgcat cgtggccggc
     1681 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     1741 gatcgggcct gccaccatac ccacgccgaa acaagcgccc tgcaccatta tgttccggat
     1801 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg
     1861 ctaaccgttt ttatcaggct ctgggaggca gaataaatga tcatatcgtc aattattacc
     1921 tccacgggga gagcctgagc aaactggcct caggcatttg agaagcacac ggtcacactg
     1981 cttccggtag tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc
     2041 tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat
     2101 tatcacttat tcaggcgtag caaccaggcg tttaagggca ccaataactg ccttaaaaaa
     2161 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     2221 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     2281 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     2341 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     2401 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2461 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     2521 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     2581 caccgtcttt cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt
     2641 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     2701 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2761 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2821 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     2881 ttatttcatt atggtgaaag
//
