LOCUS       sequence-24555-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Yasuda R, Harvey CD, Zhong H, Sobczyk A, van Aelst L, Svoboda K
  TITLE     Supersensitive Ras activation in dendrites and spines revealed by 
            two-photon fluorescence lifetime imaging.
  JOURNAL   Nat Neurosci. 2006 Feb . 9(2):283-91.
  PUBMED    16429133
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2006 Feb . 9(2):283-91."
COMMENT     Sequence Label: mEGFP-HRas S17N
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          155..287
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     270..289
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        332..350
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      366..375
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(417..438)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cgnngggcat tagcagagct gttagtgaac cgtcagatca ctagaagctt tattgcggta
       61 gtttatcaca gnttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt
      121 aagctgcaga agttggtcgt gaggcactgg gcaggtaagt atcaaggtta caagacaggt
      181 ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc gtttctgata
      241 ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg tccactccca
      301 gttcaattac agctcttaag gctagagtac ttaatacgac tcactatagg ctagcgctac
      361 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      421 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      481 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
      541 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
      601 accacatgaa gcagcacgac ttcttcannn ccgccatgcc cgaaggctac gtcntggaac
      661 gccacattct tctttcaagg acgacggcaa ctacaanacc cgcgccgagt gaattnnnga
      721 gggcgacacc ctggtgaacc gcatcgagct gaaggcatcg acttcaagga agacgnnact
      781 tcttgggnca aagctggagt aatctactac nccaccccct ctttnnttnc tgncganann
      841 aaagacgggt caagtnaa
//
