LOCUS       sequence-245552-        1031 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RAP2A in mammalian cells in a G418-selectable plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Walton ZE, Patel CH, Brooks RC, Yu Y, Ibrahim-Hashim A, Riddle M, 
            Porcu A, Jiang T, Ecker BL, Tameire F, Koumenis C, Weeraratna AT, 
            Welsh DK, Gillies R, Alwine JC, Zhang L, Powell JD, Dang CV
  TITLE     Acid Suspends the Circadian Clock in Hypoxia through Inhibition of 
            mTOR.
  JOURNAL   Cell. 2018 Jun 28;174(1):72-87.e32. doi: 10.1016/j.cell.2018.05.009.
            Epub 2018 May 31.
  PUBMED    29861175
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.05.009"; journalName: "Cell"; date: 
            "2018-06-28- 28"; volume: "174"; issue: "1"; pages: "72-87.e32"
COMMENT     Sequence Label: pcDNA3.1+ Flag-RAP2A
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             104..127
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(764..781)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    770..994
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgctaccggt atggactaca aggatgacga
      121 tgacaagggt ggtggtggtg gtgcgtcgac gatgcgcgag tacaaagtgg tggtgctggg
      181 ctcgggcggg gtaggcaaat ccgccctgac cgtgcagttc gtgaccggca ccttcatcga
      241 gaaatacgac cccaccatcg aggacttcta ccgcaaggag atcgaggtgg attcgtcgcc
      301 gtcggtgctg gagatcctgg acacggcggg caccgagcag ttcgcgtcca tgcgggacct
      361 gtacatcaag aacggccagg gcttcatcct cgtctacagc ctcgtcaacc agcagagctt
      421 ccaggacatc aagcccatgc gggaccagat catccgcgtg aagcggtatg agaaagtgcc
      481 agtcatcttg gttgggaaca aagtggacct ggaaagtgag agagaagtat cgtccagcga
      541 aggcagagcc cttgctgaag agtggggctg cccctttatg gaaacttccg ctaagagtaa
      601 aacaatggtg gacgaactct ttgcagaaat tgtgaggcag atgaactatg ctgctcagcc
      661 tgacaaagat gacccatgct gttctgcatg taacatacaa taggatatcc agcacagtgg
      721 cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
      781 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
      841 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
      901 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
      961 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     1021 ggctctaggg g
//
