LOCUS       sequence-24557-e         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Yasuda R, Harvey CD, Zhong H, Sobczyk A, van Aelst L, Svoboda K
  TITLE     Supersensitive Ras activation in dendrites and spines revealed by 
            two-photon fluorescence lifetime imaging.
  JOURNAL   Nat Neurosci. 2006 Feb . 9(2):283-91.
  PUBMED    16429133
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2006 Feb . 9(2):283-91."
COMMENT     Sequence Label: mEGFP-HRas G12V
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..32
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          147..279
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     262..281
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        324..342
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      358..367
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(409..430)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttagcagagc tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc
       61 acagttaaat tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca
      121 gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag
      181 accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta
      241 ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt
      301 acagctctta aggctagagt acttaatacg actcactata ggctagcgct accggtcgcc
      361 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      421 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
      481 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      541 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
      601 aagcagcacg acttcttcaa gtccgccatg c
//
