LOCUS       sequence-245571-        1228 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses constitutively GTP-bound RAGB in mammalian cells in a 
            G418-selectable plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Walton ZE, Patel CH, Brooks RC, Yu Y, Ibrahim-Hashim A, Riddle M, 
            Porcu A, Jiang T, Ecker BL, Tameire F, Koumenis C, Weeraratna AT, 
            Welsh DK, Gillies R, Alwine JC, Zhang L, Powell JD, Dang CV
  TITLE     Acid Suspends the Circadian Clock in Hypoxia through Inhibition of 
            mTOR.
  JOURNAL   Cell. 2018 Jun 28;174(1):72-87.e32. doi: 10.1016/j.cell.2018.05.009.
            Epub 2018 May 31.
  PUBMED    29861175
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.05.009"; journalName: "Cell"; date: 
            "2018-06-28- 28"; volume: "174"; issue: "1"; pages: "72-87.e32"
COMMENT     Sequence Label: pcDNA3.1+ Flag-RAGB99L
FEATURES             Location/Qualifiers
     source          1..1228
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             104..127
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgctaccggt atggactaca aggatgacga
      121 tgacaagggt ggtggtggtg gtgcgtcgac gatggaagaa tctgactctg agaaaacgac
      181 ggagaaagaa aatctggggc cgagaatgga tccaccacta ggggaaccgg aaggatcgct
      241 tgggtgggtg ctaccaaata cagccatgaa gaaaaaggtg ctgttgatgg gtaaaagtgg
      301 gtctggtaag accagcatga ggtctattat ctttgcaaat tatattgcca gagacacacg
      361 tcgccttggc gcaacaattg atgtagaaca ttctcatgtt cgatttctgg gaaacctggt
      421 attgaacctg tgggattgtg gtgggctaga caccttcatg gaaaattatt tcactagcca
      481 acgggacaac atcttccgaa atgtggaggt tctgatttat gtctttgatg tggagagccg
      541 cgaactggaa aaggacatgc actattacca atcatgcctg gaggccattc tgcagaattc
      601 tccagatgcc aaaatatttt gcttggtaca caaaatggat ctggtacagg aggatcaacg
      661 ggacctgatt tttaaagagc gagaagaaga tttgaggcgt ttgtctcgcc cattggaatg
      721 ttcttgtttc cgaacatcta tctgggatga aaccctctat aaggcttggt ccagcatagt
      781 ttatcagctg attcccaatg ttcagcagct ggaaatgaac ctaaggaatt ttgctgaaat
      841 tatcgaagct gatgaagtac ttctttttga gagagctact tttctggtaa tttctcacta
      901 tcagtgtaaa gagcagcgtg atgcccatag atttgagaaa ataagcaaca ttattaagca
      961 gttcaagctg agctgcagca agctggctgc ctctttccag agtatggaag tcaggaactc
     1021 taacttcgct gctttcattg acatctttac atccaacact tatgtgatgg ttgtgatgtc
     1081 tgatccgtcc attccttctg cagctactct gatcaacatc cgcaatgcca ggaaacactt
     1141 tgaaaagctg gaaagagtgg atggaccaaa gcagtgtctt ctcatgcgct aagatatcca
     1201 gcacagtggc ggccgctcga gtctagag
//
