LOCUS       sequence-245577-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  constitutive expression of a guide RNA targeting human WRN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Chan EM, Shibue T, McFarland JM, Gaeta B, Ghandi M, Dumont N, 
            Gonzalez A, McPartlan JS, Li T, Zhang Y, Bin Liu J, Lazaro JB, Gu P,
            Piett CG, Apffel A, Ali SO, Deasy R, Keskula P, Ng RWS, Roberts EA, 
            Reznichenko E, Leung L, Alimova M, Schenone M, Islam M, Maruvka YE, 
            Liu Y, Roper J, Raghavan S, Giannakis M, Tseng YY, Nagel ZD, 
            D'Andrea A, Root DE, Boehm JS, Getz G, Chang S, Golub TR, Tsherniak 
            A, Vazquez F, Bass AJ
  TITLE     WRN helicase is a synthetic lethal target in microsatellite unstable
            cancers.
  JOURNAL   Nature. 2019 Apr 10. pii: 10.1038/s41586-019-1102-x. doi: 
            10.1038/s41586-019-1102-x.
  PUBMED    30971823
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2019 Apr 10. pii: 10.1038/s41586-019-1102-x. doi: 
            10.1038/s41586-019-1102-x."
COMMENT     Sequence Label: pXPR_BRD003-sgWRN2
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    91..112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(127..132,133..150,151..157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    165..181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     170..192
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     189..205
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     189..205
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     224..244
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        226..244
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        272..498
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             499..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    726..851
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1344..1577
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1762..1806
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1984..2232
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1984..2004
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2155..2174
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2254..2329
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2385..2502
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2641..3819
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2872..3810
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3767..3787
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3832..4431
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3832..3851)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4328..4348
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4447..5035
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4500..4520)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4918..4929)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5107..5340
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5418..5539
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5455..5474)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5509..5528
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5579..5714
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5734..5753)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5735..5753)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5763..5780)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5763..5779)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5772..5794)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5921..6376
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6008..6027)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6218..6239
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6402..6506
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6507..6575,6576..7367)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6725..6744)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7538..8126
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8027..8046
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8280..8297
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
       61 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
      121 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
      181 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
      241 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
      301 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
      361 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
      421 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
      481 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
      541 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
      601 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
      661 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
      721 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
      781 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
      841 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
      901 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
      961 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     1021 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     1081 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     1141 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     1201 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     1261 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     1321 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     1381 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     1441 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     1501 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     1561 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     1621 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     1681 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     1741 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     1801 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     1861 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     1921 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     1981 agagagggcc tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga
     2041 gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt
     2101 agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat
     2161 catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     2221 ggacgaaaca ccgatcctgt ggaacatacc atggttttag agctagaaat agcaagttaa
     2281 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttaagct
     2341 tggcgtaact agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     2401 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2461 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     2521 agcagagatc cactttggcg ccggctcgag ggggcccggg tgcaaagatg gataaagttt
     2581 taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt
     2641 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     2701 ggaggggtcg gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     2761 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     2821 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     2881 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     2941 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     3001 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     3061 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     3121 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     3181 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     3241 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3301 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3361 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3421 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3481 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3541 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3601 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3661 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3721 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3781 gtggttcaaa gtttttttct tccatttcag gtgtcgtgac gtacggccac catgaccgag
     3841 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     3901 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     3961 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     4021 gtgtgggtcg cggacgacgg cgccgccgtg gcggtctgga ccacgccgga gagcgtcgaa
     4081 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     4141 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     4201 ttcctggcca ccgtcggagt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     4261 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     4321 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     4381 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aacgcgttaa
     4441 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     4501 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     4561 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     4621 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     4681 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     4741 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4801 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     4861 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4921 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4981 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     5041 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     5101 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     5161 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5221 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5281 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5341 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     5401 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5461 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5521 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     5581 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5641 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     5701 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     5761 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5821 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5881 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     5941 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6001 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6061 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6121 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6181 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6241 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6301 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     6361 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     6421 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     6481 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     6541 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     6601 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     6661 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     6721 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     6781 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     6841 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     6901 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     6961 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7021 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     7081 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     7141 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7201 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7261 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7321 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     7381 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     7441 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     7501 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7561 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7621 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7681 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7741 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7801 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7861 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7921 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7981 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8041 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8101 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8161 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8221 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8281 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caa
//
