LOCUS       sequence-24559-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Harvey CD, Ehrhardt AG, Cellurale C, Zhong H, Yasuda R, Davis RJ, 
            Svoboda K.
  TITLE     A genetically encoded fluorescent sensor of ERK activity
  JOURNAL   PNAS 2008 Dec 9;105(49):19264-9
  PUBMED    19033456
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PNAS"; 
            date: "2008-12-9"; volume: "105(49)"; pages: "19264-"
COMMENT     Sequence Label: cytoplasmic EKAR (Cerulean-Venus)
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        238..256
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     238..255
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     269..288
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      344..353
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(350..352,353..355,356..868)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(395..416)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(656..675)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcagagctcg tttagtgacc gtcagatcgc ctggagacgc catccacgct gttttgacct
       61 ccatagaaga caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg
      121 gattccccgt gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt
      181 ggcttcgtta gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt
      241 taggtgacac tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag
      301 gtccaactgc acctcggttc tatcgattga attcgtaccg gtcgccacca tggtgagcaa
      361 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      421 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      481 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      541 cctgacctgg ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
      601 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      661 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      721 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      781 caacgccatc agcgacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc
      841 caacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      901 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctga
//
