LOCUS       sequence-245592-       10617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains Blue Fluorescent Protein insert and a lineage-specific 
            human surfactant protein C (SPC) promoter driving BFP expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10617)
  TITLE     Kotton lab lentiviral vectors
REFERENCE   2  (bases 1 to 10617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-hSPC-TagBFP-W
FEATURES             Location/Qualifiers
     source          1..10617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2396..2418
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2410..2427
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2411..2427
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6297..6998
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    7007..7595
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7060..7080)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7478..7489)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7670..7903
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8022..8040)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8108..8212
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8213..8281,8282..9073)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8431..8450)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9244..9832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9733..9752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9986..10003
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10243..10264
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10279..10284,10285..10302,10303..10309)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10317..10333
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10322..10344
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10341..10357
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10341..10357
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10407..10426
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtatg
     2221 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     2281 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     2341 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     2401 tcacgacgtt gtaaaacgac ggccagtgcc aagcttgaag actgctgctc tctaccacgt
     2461 tagctcccct gtggcggagg atgactgtcc taagagctca taggcacggg gcaaggggag
     2521 cctggctgtc agctgcctgg gcttctagtc ttgtgctttt tgcacaccag tccagggaac
     2581 gaagaccact ggctttaaga tgcttcccca gctgtcccca gactctgcca gcaggggatt
     2641 ctctggtctg agcttaagtt gtgttctccc agccagggat gcccctgccc tttgatgtct
     2701 ccttgctgcc acacatttag ccgccctccc catgccagct tggggggagg gaagcagtgg
     2761 gggtagggag gtggctgggg cagctgggca actgtcccca cccgtccctg gcacggctct
     2821 gcccagtaca caaagagcaa agtgaatctt gtccccaccc ctgcagctga ggggctggag
     2881 gaggaaacgg ggaggcccac acagaagggg tggccaccgt ggggctgtcc atcactcagg
     2941 gctctcagag ggagtcaacc cagaaacaga caaagagggt gagtctgggc tgtgttctta
     3001 gctagtgaga ggtcccctag aggatgaagt agatgatgct aatgaggatg actggatgtc
     3061 acacccatga tgctattagg tcctcataat agcatagtga ggtggacagc tagtacctga
     3121 cccatctcac agatgaacat actaatgcct aacaaagcag aacgactcac gctgggtccc
     3181 agagctggcc agtggaagca ctgagacctc cacatactga aggcatggac tattgaccgc
     3241 tgttggtatt ggtctcatca ttgactatca ttaagtgttg gctgtttgca tgcttcctgc
     3301 ccagtggcag gttcaaagaa gcccgcagga agcgtgctcc tttctttccc agggcccgca
     3361 attgggctgg aagatagagc aacaaaaagc gcccatgtaa ctcatgggaa cattcatgtg
     3421 tgctgaatgg caggtgaagg tgccacagag aggctgagga tttcagaggg caccatgaac
     3481 tggagtgagg tcgcagagca ggtgccattg gctcttggcc tgtttgggtg ggtggcattc
     3541 agagaggtgg aaggtcagat gcactgttca cgcctgtaat ctcagcactt tggaaggcca
     3601 aggtaggagg atcacacgag gccaggagat caacgctgca gtgagctatg aagctgtgat
     3661 tgcaccactg cactgcagct tgggtaacag agtgagaccc tgtctctaaa taattaaata
     3721 aataaaataa aaataaaacc ggagaagtgg agagggattg gaggtgggct ttcacagagg
     3781 gagaaacggc ttaagtacag gccaaaaagt gagaaggctg cagacagggc tggtaggggg
     3841 agggggaaat ttggcacacc cagctaaagg tccttctgtg tctccttctc caaggaaccc
     3901 aagaccttca cttggttggt gtgagcactc caggaggcag gcaccctccc tcagccctca
     3961 agcaagcaaa aatgggttta aaaaaagaag gagaagaagc agcagcagca gccgccacag
     4021 agcttgtgac agctacagcc taagggcaac aggcagggga gaccaaggac cagaaagagc
     4081 agaggctttt tcaaagaaag agatccctct cccagcaccc agcgatggcg gcaaacccca
     4141 cccacagtgc ctgctaagaa caagtcccac gtgagaacaa catggccccc cgagatgccc
     4201 acagggaccc cgagatgcct gcagtgcttg gctctcccgc tggccagctg cccacctggc
     4261 tcaggcccag tactcgtgag tcagccgatc aatcccaatg ttgccaggat gatgggggcg
     4321 ggagtaaggg ccctggggga gggcaggggt gggcactgca ggcgagctgt ctcccacatc
     4381 tggcacctgc acacagctga ggccgagctg agaggatgct tctgtgggct ccccctcctc
     4441 ccggcacccc tcccctcctt tcactgtcct aggacactct ctggctgctg gagtcttagg
     4501 caaatattta aaggggcagc aagggggtga ggagggtggt gggagcaaac acttccctcc
     4561 ctttcttcct ccctgcgctt ctcaggggct ctcagttcag atgccatgct gttatgcaac
     4621 cttggggctg aaggccctcc agatggagag ggggacaggg gaacctgcca gctcatgacc
     4681 gaagggcagg gcccaggtgg gagggggctg gggcagggga caggaactgg ggtggcatgt
     4741 taaaggacag gaggctggtt gggcatggtg gctcacacct gtaatcctag cactttagga
     4801 ggccgagatc acttgagccc aggagttcaa gaccagcctg ggcaacatgg tgaaaactca
     4861 tctctataaa acaagcaaaa attagctggg cacaatggca tgcactggta gtcccagcta
     4921 cttgggatgc tgaggtgtga ggatcaccgg agtccaggag gtcaacgctg cagtgagcag
     4981 tgatctcgct actgcatgcc agcctgggtg ataaagtgag accctgtctc acaacaaaac
     5041 aaaacaaaac aaaacaaaag gataggaggt taagggagcg agcccaggcc tggactctgc
     5101 cacagtcacc tgagtttaga ggtgaaggga ctttagagac cacctggccc aaggagtgaa
     5161 agggaacctg agagaaaggg tgagccagcc caaagtcatt ggcagatttg acctcgtaaa
     5221 tacatagaga tggctttggg aaggcactag gaaagacaga gaaaagagaa ggagacagtc
     5281 ctcaaagctg atcgtatttg ggtgagtatt attctcaggg caaatttagg atcagaggat
     5341 gcagaaaggg gagtctagag gggtagagtg tagaccacag ggtgagtgag ctgattcgag
     5401 gatggggaga ctgggagccc accagtgacc agagccagcc ctgttcaggg ctgtccgggc
     5461 agaagaaagc agtgtcagac ctggaatctg ccatcagcac agcctgcaat tgacagacaa
     5521 gcccagagca aagaaggaag cactgcacat gagtaagagc ttgccaccag tggggacaga
     5581 gtttccagaa ttaggaaaat aatcactggg ggcaagtttg aggttggtac cagatatgtg
     5641 ggaggaggca aggtaaggga aagagtactt gaagttggaa ctggtccttg cagggaaatg
     5701 cacatttatg aaaccccgaa aactgatgtc aaagcacctc ctgccttggg cagagtcctc
     5761 tcagagtcta caggtgctgc ctccagaacc ctcttcctgg agcgcatccc tatgtatcta
     5821 gaaattctgc tgggaaatat gatggtcaga cccttggcca cctgaaaggt tcagggtggt
     5881 agaagaaaaa ggaaagccac agggcagcag gggcaggtgc cagcaaggaa ggcaggcacg
     5941 ccaggaagac acccatggtg agaagtgcag atggcccgag ggcaagtttg ctcaactcac
     6001 ccaggtttgc tcttgctggg gccaagagga ctcatgtgcc agggccaagg gcccttgggg
     6061 gctctcacag ggggcttatc tgggcttcgg ttctggaggg ccaggaacaa acaggcttca
     6121 aagccaaggg cttggctggc acacaggggg cttggtcctt cacctctgtc ccctctccct
     6181 acggacacat ataagaccct ggtcacacct gggagaggag gagaggagag catagcacct
     6241 gcaggtcgac tctagaggat cgatccccgg gtaccgagct cgaattagcg gccgccatga
     6301 gcgagctgat taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc
     6361 atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga
     6421 gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct
     6481 tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc
     6541 agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc
     6601 tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca
     6661 gaggggtgaa cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg
     6721 ccttcaccga gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc
     6781 tgaagctcgt gggcgggagc catctgatcg caaacatcaa gaccacatat agatccaaga
     6841 aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa
     6901 tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact
     6961 gcgacctccc tagcaaactg gggcacaagc ttaattaaag gatcctaatc aacctctgga
     7021 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7081 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7141 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7201 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7261 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7321 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7381 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     7441 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7501 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7561 gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag accaatgact
     7621 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7681 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     7741 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7801 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7861 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     7921 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggcccggg
     7981 ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga tacgcctatt
     8041 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     8101 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     8161 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     8221 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     8281 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     8341 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8401 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
     8461 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     8521 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8581 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8641 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8701 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8761 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8821 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8881 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8941 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     9001 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9061 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9121 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9181 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9241 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9301 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9361 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9421 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9481 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9541 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9601 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     9661 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9721 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9781 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9841 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9901 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9961 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
    10021 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca agctcatggc
    10081 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
    10141 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc cgtggcacga
    10201 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
    10261 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
    10321 gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt tcacaaataa
    10381 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
    10441 tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc ccaccccata
    10501 ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg attcctctga
    10561 attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact tagtagt
//
