LOCUS       sequence-24561-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Harvey CD, Ehrhardt AG, Cellurale C, Zhong H, Yasuda R, Davis RJ, 
            Svoboda K.
  TITLE     A genetically encoded fluorescent sensor of ERK activity
  JOURNAL   PNAS 2008 Dec 9;105(49):19264-9
  PUBMED    19033456
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PNAS"; 
            date: "2008-12-9"; volume: "105(49)"; pages: "19264-"
COMMENT     Sequence Label: nuclear EKAR (Cerulean-Venus)
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        249..267
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     249..266
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     280..299
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      355..364
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(406..427)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     gap             688..698
                     /estimated_length=11
ORIGIN
        1 gngggnnatt agcagagctc gttagtgaac cgtcagatcg cctggagacg ccatccacgc
       61 tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc
      121 attggaacgc ggattccccg tgccaagagt gacgtaagta ccgcctatag agtctatagg
      181 cccaccccct tggcttcgtt agaacgcggc tacaattaat acataacctt atgtatcata
      241 cacatacgat ttaggtgaca ctatagaata acatccactt tgcctttctc tccacaggtg
      301 tccactccca ggtccaactg cacctcggtt ctatcgattg aattcgtacc ggtcgccacc
      361 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      421 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      481 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      541 ctcgtgacca ccctgacctg gggcgtgcag tgcttcgccc gctaccccga ccacatgaag
      601 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      661 ttcaaggacg acggcaatca ccangcgnnn nnnnnnnntt ggnnnnnnnn aacccttgna
      721 acncntnatc gagctgaagg cttcactntt caaggaggac ggcaacatcc tggggcacan
      781 gctggagtac acgccatcag cgacaacgtc tatntcac
//
