LOCUS       sequence-24562-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Harvey CD, Ehrhardt AG, Cellurale C, Zhong H, Yasuda R, Davis RJ, 
            Svoboda K.
  TITLE     A genetically encoded fluorescent sensor of ERK activity
  JOURNAL   PNAS 2008 Dec 9;105(49):19264-9
  PUBMED    19033456
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PNAS"; 
            date: "2008-12-9"; volume: "105(49)"; pages: "19264-"
COMMENT     Sequence Label: nuclear EKAR (EGFP-mRFP)
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          152..284
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     267..286
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        329..347
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(483..501)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 cnnggncntt agcagactgt tagtgaaccg tcagatcact agaagcttta ttgcggtagt
       61 ttatcacagt taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag
      121 ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta
      181 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc
      241 acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccagtt
      301 caattacagc tcttaaggct agagtactta atacgactca ctataggcta gcatggcctc
      361 ctccgaggac gtcatcaagg agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa
      421 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
      481 cgccaagctg aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc
      541 tcagttccag tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
      601 gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
      661 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
      721 gctgcgcggc accaacttcc cctccgacgg ccccgtaatg canaagaaga ccatgggctg
      781 ggaggcctcc accgagcgga tgtaccccga ggacgg
//
