LOCUS       sequence-245647-        7743 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. malariae protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Pm_P41-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2521..2538
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2590..2645
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2644..2663)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4881..4899)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4967..5071
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5072..5140,5141..5932)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5290..5309)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6103..6691
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6592..6611
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6845..6862
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6979..7000
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7015..7020,7021..7038,7039..7045)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7053..7069
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7058..7080
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7077..7093
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7077..7093
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7364..7743
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gcccaggaac acgtctgcga cttcaccaaa
      841 gagaagtacc tgctgggcaa gaacgagaaa gaatactgcg aggtgcacgc caaccccttc
      901 gacagcgtga ccttcatctg ccccgagaag atcggagccg agtgcttcca gaacgtgaac
      961 atcgtggacg acatcacaaa agaaaagatg gacagcagca agatcagcat cgacgagatc
     1021 ctgtacggca gcaccgtgta cggcgacacc ctgctgatct ccccaaccgt gaagaagaat
     1081 accgccttct actgcttctg caacctgcag accgccgacg tgcagaagta tctgcaggaa
     1141 cggaaggccg agaaagagaa gctgatcaaa gaaagcaaga gcatcgtggt cgaggacagc
     1201 gaagaggaag aggtgctggt caaggaagaa gaagaggaac agcagaaggt ggacgagcac
     1261 ctggaaaagg ccctgcagag agtgaagaag tacaagaaca tcatcgaaaa agagaagaag
     1321 aaagactccg gcgagagccc cgaggacaac gaggaaatcg tcgaggaaga agtggacgaa
     1381 gagatcaaga tcaagacaga gaagatcatc accaaatacg gcatcatgaa ggtggtggtg
     1441 tccaccagcg acaccatcac caagggctgc gatttcggca acaacgtggt caactacttc
     1501 agcaacccct accccgtcga gaagtacggc ggcagcagag tgtgcagaat cgaggccaag
     1561 cctggcgagt tcgtgggctt caagtgcatc tacgacaaca ccggcaacgt ggaacccaac
     1621 aactgcttcg acaaggtgct gtacgaggac aaagagacag acctgaaaac cctgatgccc
     1681 ggctacatca gctacggcaa caagcggcag gggaagtacg ccttttacct gaagctgccc
     1741 cacttcatcc agcacaacta cgccatccgg tgcaagtgca gatccaccgt ggcccagtac
     1801 gacaactacg tgttcgagct ggccgtggaa ggcggcgagt ctgccgccat cagccagacc
     1861 tttcaggatg gcgcgccgtc gacctccatc acggcctata agagtgaggg ggagtcagcg
     1921 gagttctcct tcccactcaa ccttggagag gaaagcctgc agggagagtt gagatggaag
     1981 gcagagaagg ctccttcttc ccagtcctgg atcaccttct ccctaaagaa ccaaaaggtg
     2041 tctgtgcaga agtctactag caaccccaag ttccagctgt ccgaaacgct cccactcacc
     2101 cttcagatac cccaggtctc ccttcagttt gctggttctg gcaacctgac cctgactctg
     2161 gacagaggga tactgtatca ggaagtgaac ctggtggtga tgaaagtgac tcagcccgac
     2221 agcaacactt tgacctgtga ggtgatggga cccacctcac ccaagatgag actgatcttg
     2281 aagcaggaga atcaggaggc cagggtctcc aggcaggaga aagtgattca agtgcaggcc
     2341 cctgaagcag gggtgtggca atgtctactg agtgaaggtg aagaggtcaa gatggactcc
     2401 aagatccagg ttttatccaa agggttgaat tccggcagcc tgcaccacat cctggacgcc
     2461 cagaagatgc tgtggaacca cagggacagg aacctgcctc ctctggcccc tctgggcccc
     2521 caccaccatc accaccattg agtctagaca cgtgattaat taaggatccc ccgacctcga
     2581 cctctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
     2641 tctcactcgg aaggacatat gggagggcaa atcatttggt cgagatccct cggagatctc
     2701 tagctagagg atcgatcccc gccccggacg aactaaacct gactacgaca tctctgcccc
     2761 ttcttcgcgg ggcagtgcat gtaatccctt cagttggttg gtacaacttg ccaactgggc
     2821 cctgttccac atgtgacacg gggggggacc aaacacaaag gggttctctg actgtagttg
     2881 acatccttat aaatggatgt gcacatttgc caacactgag tggctttcat cctggagcag
     2941 actttgcagt ctgtggactg caacacaaca ttgcctttat gtgtaactct tggctgaagc
     3001 tcttacacca atgctggggg acatgtacct cccaggggcc caggaagact acgggaggct
     3061 acaccaacgt caatcagagg ggcctgtgta gctaccgata agcggaccct caagagggca
     3121 ttagcaatag tgtttataag gcccccttgt taaccctaaa cgggtagcat atgcttcccg
     3181 ggtagtagta tatactatcc agactaaccc taattcaata gcatatgtta cccaacggga
     3241 agcatatgct atcgaattag ggttagtaaa agggtcctaa ggaacagcga tatctcccac
     3301 cccatgagct gtcacggttt tatttacatg gggtcaggat tccacgaggg tagtgaacca
     3361 ttttagtcac aagggcagtg gctgaagatc aaggagcggg cagtgaactc tcctgaatct
     3421 tcgcctgctt cttcattctc cttcgtttag ctaatagaat aactgctgag ttgtgaacag
     3481 taaggtgtat gtgaggtgct cgaaaacaag gtttcaggtg acgcccccag aataaaattt
     3541 ggacgggggg ttcagtggtg gcattgtgct atgacaccaa tataaccctc acaaacccct
     3601 tgggcaataa atactagtgt aggaatgaaa cattctgaat atctttaaca atagaaatcc
     3661 atggggtggg gacaagccgt aaagactgga tgtccatctc acacgaattt atggctatgg
     3721 gcaacacata atcctagtgc aatatgatac tggggttatt aagatgtgtc ccaggcaggg
     3781 accaagacag gtgaaccatg ttgttacact ctatttgtaa caaggggaaa gagagtggac
     3841 gccgacagca gcggactcca ctggttgtct ctaacacccc cgaaaattaa acggggctcc
     3901 acgccaatgg ggcccataaa caaagacaag tggccactct tttttttgaa attgtggagt
     3961 gggggcacgc gtcagccccc acacgccgcc ctgcggtttt ggactgtaaa ataagggtgt
     4021 aataacttgg ctgattgtaa ccccgctaac cactgcggtc aaaccacttg cccacaaaac
     4081 cactaatggc accccgggga atacctgcat aagtaggtgg gcgggccaag ataggggcgc
     4141 gattgctgcg atctggagga caaattacac acacttgcgc ctgagcgcca agcacagggt
     4201 tgttggtcct catattcacg aggtcgctga gagcacggtg ggctaatgtt gccatgggta
     4261 gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat
     4321 aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat
     4381 atgctatcct aatttatatc tgggtagcat aggctatcct aatctatatc tgggtagcat
     4441 atgctatcct aatctatatc tgggtagtat atgctatcct aatctgtatc cgggtagcat
     4501 atgctatcct aatagagatt agggtagtat atgctatcct aatttatatc tgggtagcat
     4561 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcatatgc
     4621 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     4681 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
     4741 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     4801 tatcctaatc tgtatccggg tagcatatgc tatcctcatg ataagctgtc aaacatgaga
     4861 attttcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     4921 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4981 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5041 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5101 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5161 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5221 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5281 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
     5341 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5401 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5461 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5521 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5581 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
     5641 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5701 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5761 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5821 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5881 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5941 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6001 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6061 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6121 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6181 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6241 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6301 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6361 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6421 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6481 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6541 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6601 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6661 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6721 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6781 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     6841 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     6901 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     6961 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     7021 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     7081 aaacagctat gaccatgatt acgccaagct ctagctagag gtcgaccaat tctcatgttt
     7141 gacagcttat catcgcagat ccgggcaacg ttgttgccat tgctgcaggc gcagaactgg
     7201 taggtatgga agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt
     7261 tatatagcat aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat
     7321 atgtacattt atattggctc atgtccaata tgaccgccat gttgacattg attattgact
     7381 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
     7441 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     7501 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     7561 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
     7621 agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
     7681 atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     7741 atg
//
