LOCUS       sequence-245649-        7632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. malariae protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 7632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Pm_P12-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2790..2807
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2859..2914
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2913..2932)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5150..5168)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5236..5340
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5341..5409,5410..6201)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5559..5578)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6372..6960
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6861..6880
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7114..7131
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7248..7269
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7284..7289,7290..7307,7308..7314)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7322..7338
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7327..7349
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7346..7362
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7346..7362
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gcctacaaga
     1201 ccaacgacga ctgcgacttc acccgcgagc ccctgaacgt ggcctggaac aacagaaaca
     1261 acgccctggc ccctgtggac atggaagatg agccctacga caacgacaac aacatcaagt
     1321 actgcgtgaa gttcaccaag ggcttcgaga tcctgacctt catctgcccc aagaagggca
     1381 tcaactacga gggcatcgag atgcggccca aagagtgctt cgagaagatc agaatcaacg
     1441 gccgggacga gaacttcgcc gagattctga agggcagcat cttcgagagc agcgagacag
     1501 acagcatcat catccggcgg gtgttcatcc ctcccaccat ctacgccgac atggtcatcg
     1561 agtgcacctg tgacaacagc ctcaccttca aagagaactt catcggcgcc agaggcatca
     1621 tgcgggtgca cctgaagcgg aacaagatct tcggctgcga tttcgacagc aacatcgacg
     1681 gcgacgacag cacctacggc agatccgatg gcggcaccag aaacaagtgc gagggccggc
     1741 tggacaacaa ctgcgaggaa agcaagagcg ggcggagcgc cttcgtgaag tactacgaca
     1801 agagcaagat ccggtccaac gaggccatca catgcaatgt ggccatcaac aagaaagagg
     1861 tgtacctggg cctgatctgc cccgagggct acacaatcta ccccagcgac tgctttgaga
     1921 acgtgctgta cgagaacaag attgtgaaca tcaaagagct gctcgtcagc cacgacatca
     1981 agctgcacat cgacaagaaa aagcggatga gcttcgccac cttcatcctg aacaagaacg
     2041 agaaccctaa gggcttcagc tgccagtgcg tgaagaacaa cgataacatc ttcccactgc
     2101 aggccaactt cgagtacgcc aattacgaga gcttctctgg cgcgccgtcg acctccatca
     2161 cggcctataa gagtgagggg gagtcagcgg agttctcctt cccactcaac cttggagagg
     2221 aaagcctgca gggagagttg agatggaagg cagagaaggc tccttcttcc cagtcctgga
     2281 tcaccttctc cctaaagaac caaaaggtgt ctgtgcagaa gtctactagc aaccccaagt
     2341 tccagctgtc cgaaacgctc ccactcaccc ttcagatacc ccaggtctcc cttcagtttg
     2401 ctggttctgg caacctgacc ctgactctgg acagagggat actgtatcag gaagtgaacc
     2461 tggtggtgat gaaagtgact cagcccgaca gcaacacttt gacctgtgag gtgatgggac
     2521 ccacctcacc caagatgaga ctgatcttga agcaggagaa tcaggaggcc agggtctcca
     2581 ggcaggagaa agtgattcaa gtgcaggccc ctgaagcagg ggtgtggcaa tgtctactga
     2641 gtgaaggtga agaggtcaag atggactcca agatccaggt tttatccaaa gggttgaatt
     2701 ccggcagcct gcaccacatc ctggacgccc agaagatgct gtggaaccac agggacagga
     2761 acctgcctcc tctggcccct ctgggccccc accaccatca ccaccattga gtctagacac
     2821 gtgattaatt aaggatcccc cgacctcgac ctctggctaa taaaggaaat ttattttcat
     2881 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     2941 tcatttggtc gagatccctc ggagatctct agctagagga tcgatccccg ccccggacga
     3001 actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc
     3061 agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca
     3121 aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc
     3181 aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat
     3241 tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc
     3301 ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag
     3361 ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt
     3421 aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct
     3481 aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa
     3541 gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg
     3601 ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca
     3661 aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc
     3721 taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg
     3781 tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta
     3841 tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac
     3901 attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat
     3961 gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca atatgatact
     4021 ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc
     4081 tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc
     4141 taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt
     4201 ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc
     4261 tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc
     4321 actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata
     4381 agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca
     4441 cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag
     4501 agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata
     4561 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     4621 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     4681 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     4741 tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata
     4801 tgctatccta atttatatct gggtagcata tactacccaa atatctggat agcatatgct
     4861 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agcataggct
     4921 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     4981 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     5041 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     5101 atcctcatga taagctgtca aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata
     5161 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     5221 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5281 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5341 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5401 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5461 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5521 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5581 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5641 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5701 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5761 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5821 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5881 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5941 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     6001 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6061 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6121 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6181 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6241 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6301 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6361 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6421 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6481 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6541 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6601 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6661 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6721 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6781 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6841 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6901 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6961 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7021 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7081 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7141 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     7201 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     7261 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     7321 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
     7381 tagctagagg tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
     7441 tgttgccatt gctgcaggcg cagaactggt aggtatggaa gatctataca ttgaatcaat
     7501 attggcaatt agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc
     7561 cattgcatac gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat
     7621 gaccgccatg tt
//
