LOCUS       sequence-24565-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Harvey CD, Yasuda R, Zhong H, Svoboda K
  TITLE     The spread of Ras activity triggered by activation of a single 
            dendritic spine.
  JOURNAL   Science. 2008 Jul 4. 321(5885):136-40.
  PUBMED    18556515
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jul 4. 321(5885):136-40."
COMMENT     Sequence Label: paGFP-HRas G12V
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(71..73,74..76,77..589)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(116..137)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(377..396)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cngatgattg agctagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc
       61 ggtcgccatn atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
      121 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
      181 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
      241 ctggcccacc ctcgtgacca ccttcagcta cggcgtgcag tgcttcagcc gctaccccga
      301 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
      361 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
      421 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
      481 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
      541 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt
      601 gcagctcgcc gaccactacc agcagaacac ccatgtgtan naggcccccg tggtgctgcc
      661 cgacanccac tacctgagnc accagtccan gtgaacaaga cccaaacgag aacgcaatca
      721 nttgggtcct gcttgnattt cntnaccccc ccccgggntn actctcggca tggacgactg
      781 tacaagtccg gactcganat gacggaatat anctggtggt ggtggcccct tgnngggnaa
      841 aaggcctnaa ctccttttac naaatttttg naaa
//
