LOCUS       sequence-245650-        8814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant P. ovale protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8814)
  AUTHORS   Muller-Sienerth N, Shilts J, Kadir KA, Yman V, Homann MV, Asghar M, 
            Ngasala B, Singh B, Farnert A, Wright GJ
  TITLE     A panel of recombinant proteins from human-infective Plasmodium 
            species for serological surveillance.
  JOURNAL   Malar J. 2020 Jan 17;19(1):31. doi: 10.1186/s12936-020-3111-5.
  PUBMED    31952523
REFERENCE   2  (bases 1 to 8814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Malar J."; 
            date: "2020-01-17"; volume: "19(1):31. doi"; pages: " 
            10.1186/s12936-020-3111-5"
COMMENT     Sequence Label: Po_MSP3.6-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3972..3989
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4041..4096
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4095..4114)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6332..6350)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6418..6522
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6523..6591,6592..7383)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6741..6760)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7554..8142
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8043..8062
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8296..8313
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8430..8451
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8466..8471,8472..8489,8490..8496)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8504..8520
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8509..8531
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8528..8544
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8528..8544
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccaaggacg
     1201 tggacaatat ccacaacgcc gccaacaaca acaagggcaa gcccaacctg cggaacggct
     1261 tcagcttcaa gaacttcttt ccacagaacg agcagaacga cctggaagag gaagatgacg
     1321 acgacgagta caacaccgac gagaacaatg ccgagatcat tgccaacggc gccaaagaga
     1381 aggccgagaa ggtcgaggaa gccaagaacg ccggcgagaa ggccatcaag agagccaaag
     1441 aggccaccta cagcgccatc gagaaagtgc ggaacgcccg ggatgccatt gaagaggcca
     1501 acaaggccgt gaaagagaaa gaacagaacg ccaaagaaaa agtgaccaag gccgaaaagg
     1561 acaccaacga cgccatggaa gatgctaaca aagccaacaa agctgccgag attatcaagc
     1621 agatcaaggc cgccattgag gccgagagcg acgaggccaa gtacaacgtg gccctggaaa
     1681 acatgctgaa gaaagtgaag tccaaggcca aagaagccga gatcgaggcc gaaatcgaag
     1741 ctgagatccc caaggccctg gccgccaaaa cagaggccct gatcgccaag atcgacgccg
     1801 agaaagccaa aaacgaagcc gtgaatatcg ccaaaaaggc caaggatgcc gtggaaaagg
     1861 cccaggtggc cgctaagaag gctaaagagg ccgccaatac cgccaccgaa cacgccctgc
     1921 aggctgagaa ggctgaaaaa gaggaaaaga ccaccgagac actggaaaaa gtcaagaaca
     1981 ttgccgacgc cgccgtggga gccgctggcg aggctaaaaa agctaagctg agcgccgaga
     2041 ttggcctgaa gctgaccaaa gcccaggaag ctaaagaaga ggccgaagaa gcagaagagg
     2101 ctgtcgaggc tgtgaacgcc gcaaaagaag aagtgaacaa agcagagcag accaccgccg
     2161 aagagacagc caacgaggtg gccaagagcg tggtcaagat cgcccagacc aaagtgacaa
     2221 aagccattga gaacgccgct gccaaggcaa aggacgccgc ctctgccgca gagaaggcaa
     2281 agaccatcga aaccctgccc gaagtgaccg ccgaggccca gacagctaag aatgccgccc
     2341 acgaggcaaa caaagtgaaa gagcgggctg agatcgccgc caagattgca aaagaggtgg
     2401 caaaggccga agtggcccgg aagaaggcca agctggctaa ggaagccgcc gacaaggcta
     2461 aggatgaggt ggaacggatc gccagagccc ctaactccgc cgagaagatt aagcaggccg
     2521 ctaacgaggc tctgcaggcc gcaaagagcg ccgaaacaaa agaaaaggaa gtggaagtca
     2581 aggctatcga cgccttcgaa gaggcaaaga aagagaagta taagaccgag gccgaggtcg
     2641 ccacagaagt ggccaaaatc gagggcgcca agaccgagac agagagcctg ctgatcaagg
     2701 ctcaggccga taaggccgtg gccaccgcca cagccgtcga gaaggtggaa acagccaccg
     2761 ccggcaccaa gaactacaaa gaaaaggcca gcctggtgga cgccgccctg aattctgcca
     2821 atgccgccgt gacacaggaa gaagaagccg tcaagcaggc cctggatatc gctatcgccg
     2881 acaaccccaa catggtggaa ctcgagtaca ccagcctggg cgagaacgag gacaccacct
     2941 accacgtgga agatgtgtcc gaggaacagg aagagggcag cgaggacgag agcaaggaca
     3001 aacagggcgt ggtgcagaag tacgaggaag aggaggaagg cgaggaagaa gaggtcgagg
     3061 aagaggacga ggaaaacgaa gaggacgagg gcgacgaaga gaaagtgaaa gaagtggtcg
     3121 agaaagtcaa cgtggcaaag ggcgagtcca aggaccagaa agaaaacaag aacgatggcg
     3181 cccctctgaa cgacgacaac gcctacacct tcctggaagg caactacaag aatgacgact
     3241 ccaccaagaa agccgacggc gccctgttcg agacaatcag cgacctggtc atgatcaccc
     3301 agcagctcct catcccttct ggcgcgccgt cgacctccat cacggcctat aagagtgagg
     3361 gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg cagggagagt
     3421 tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc tccctaaaga
     3481 accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg tccgaaacgc
     3541 tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct ggcaacctga
     3601 ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg atgaaagtga
     3661 ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca cccaagatga
     3721 gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag aaagtgattc
     3781 aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt gaagaggtca
     3841 agatggactc caagatccag gttttatcca aagggttgaa ttccggcagc ctgcaccaca
     3901 tcctggacgc ccagaagatg ctgtggaacc acagggacag gaacctgcct cctctggccc
     3961 ctctgggccc ccaccaccat caccaccatt gagtctagac acgtgattaa ttaaggatcc
     4021 cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     4081 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg tcgagatccc
     4141 tcggagatct ctagctagag gatcgatccc cgccccggac gaactaaacc tgactacgac
     4201 atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt
     4261 gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa ggggttctct
     4321 gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga gtggctttca
     4381 tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta tgtgtaactc
     4441 ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc ccaggaagac
     4501 tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat aagcggaccc
     4561 tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa acgggtagca
     4621 tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat agcatatgtt
     4681 acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta aggaacagcg
     4741 atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga ttccacgagg
     4801 gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg gcagtgaact
     4861 ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa taactgctga
     4921 gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca
     4981 gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca atataaccct
     5041 cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa tatctttaac
     5101 aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct cacacgaatt
     5161 tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat taagatgtgt
     5221 cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta acaaggggaa
     5281 agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc ccgaaaatta
     5341 aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc ttttttttga
     5401 aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt tggactgtaa
     5461 aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt caaaccactt
     5521 gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg ggcgggccaa
     5581 gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc
     5641 aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt
     5701 tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat
     5761 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     5821 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     5881 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     5941 ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat
     6001 ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     6061 gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg
     6121 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg
     6181 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     6241 gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gataagctgt
     6301 caaacatgag aattttcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     6361 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     6421 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     6481 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     6541 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     6601 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6661 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6721 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     6781 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6841 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6901 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6961 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     7021 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     7081 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     7141 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     7201 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     7261 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     7321 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     7381 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     7441 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7501 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7561 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7621 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7681 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     7741 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7801 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7861 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7921 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7981 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8041 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     8101 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     8161 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     8221 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     8281 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     8341 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     8401 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     8461 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     8521 tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgaccaa
     8581 ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg
     8641 cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat
     8701 tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc
     8761 tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca tgtt
//
