LOCUS       sequence-245655-       11782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of PDGFRA D842V.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11782)
  AUTHORS   Ng PK, Li J, Jeong KJ, Shao S, Chen H, Tsang YH, Sengupta S, Wang Z,
            Bhavana VH, Tran R, Soewito S, Minussi DC, Moreno D, Kong K, 
            Dogruluk T, Lu H, Gao J, Tokheim C, Zhou DC, Johnson AM, Zeng J, Ip 
            CKM, Ju Z, Wester M, Yu S, Li Y, Vellano CP, Schultz N, Karchin R, 
            Ding L, Lu Y, Cheung LWT, Chen K, Shaw KR, Meric-Bernstam F, Scott 
            KL, Yi S, Sahni N, Liang H, Mills GB
  TITLE     Systematic Functional Annotation of Somatic Mutations in Cancer.
  JOURNAL   Cancer Cell. 2018 Mar 12;33(3):450-462.e10. doi: 
            10.1016/j.ccell.2018.01.021.
  PUBMED    29533785
REFERENCE   2  (bases 1 to 11782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2018.01"; journalName: "Cancer Cell"; date: 
            "2018-03-12- 12"; volume: "33"; issue: "3"; pages: "450-462.e10"
COMMENT     Sequence Label: pHAGE-PDGFRA-D842V
FEATURES             Location/Qualifiers
     source          1..11782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3421..3445
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6719..6743)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6796..6837
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    6858..7425
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6875..7427
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7019..7036)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7246..7265
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7439..7441,7442..7444,7445..8158)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7439..7441,7442..7444,7445..8155)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7484..7505)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7745..7764)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8092..8113
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8172..8760
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8225..8245)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8643..8654)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8835..9068
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9187..9205)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9273..9377
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9378..9446,9447..10238)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9596..9615)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10409..10997
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10898..10917
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11151..11168
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11408..11429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11444..11449,11450..11467,11468..11474)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11482..11498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11487..11509
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11506..11522
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11506..11522
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11572..11591
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccct gcagatatca
     3421 acaagtttgt acaaaaaagc aggcaccatg gggacttccc atccggcgtt cctggtctta
     3481 ggctgtcttc tcacagggct gagcctaatc ctctgccagc tttcattacc ctctatcctt
     3541 ccaaatgaaa atgaaaaggt tgtgcagctg aattcatcct tttctctgag atgctttggg
     3601 gagagtgaag tgagctggca gtaccccatg tctgaagaag agagctccga tgtggaaatc
     3661 agaaatgaag aaaacaacag cggccttttt gtgacggtct tggaagtgag cagtgcctcg
     3721 gcggcccaca cagggttgta cacttgctat tacaaccaca ctcagacaga agagaatgag
     3781 cttgaaggca ggcacattta catctatgtg ccagacccag atgtagcctt tgtacctcta
     3841 ggaatgacgg attatttagt catcgtggag gatgatgatt ctgccattat accttgtcgc
     3901 acaactgatc ccgagactcc tgtaacctta cacaacagtg agggggtggt acctgcctcc
     3961 tacgacagca gacagggctt taatgggacc ttcactgtag ggccctatat ctgtgaggcc
     4021 accgtcaaag gaaagaagtt ccagaccatc ccatttaatg tttatgcttt aaaagcaaca
     4081 tcagagctgg atctagaaat ggaagctctt aaaaccgtgt ataagtcagg ggaaacgatt
     4141 gtggtcacct gtgctgtttt taacaatgag gtggttgacc ttcaatggac ttaccctgga
     4201 gaagtgaaag gcaaaggcat cacaatgctg gaagaaatca aagtcccatc catcaaattg
     4261 gtgtacactt tgacggtccc cgaggccacg gtgaaagaca gtggagatta cgaatgtgct
     4321 gcccgccagg ctaccaggga ggtcaaagaa atgaagaaag tcactatttc tgtccatgag
     4381 aaaggtttca ttgaaatcaa acccaccttc agccagttgg aagctgtcaa cctgcatgaa
     4441 gtcaaacatt ttgttgtaga ggtgcgggcc tacccacctc ccaggatatc ctggctgaaa
     4501 aacaatctga ctctgattga aaatctcact gagatcacca ctgatgtgga aaagattcag
     4561 gaaataaggt atcgaagcaa attaaagctg atccgtgcta aggaagaaga cagtggccat
     4621 tatactattg tagctcaaaa tgaagatgct gtgaagagct atacttttga actgttaact
     4681 caagttcctt catccattct ggacttggtc gatgatcacc atggctcaac tgggggacag
     4741 acggtgaggt gcacagctga aggcacgccg cttcctgata ttgagtggat gatatgcaaa
     4801 gatattaaga aatgtaataa tgaaacttcc tggactattt tggccaacaa tgtctcaaac
     4861 atcatcacgg agatccactc ccgagacagg agtaccgtgg agggccgtgt gactttcgcc
     4921 aaagtggagg agaccatcgc cgtgcgatgc ctggctaaga atctccttgg agctgagaac
     4981 cgagagctga agctggtggc tcccaccctg cgttctgaac tcacggtggc tgctgcagtc
     5041 ctggtgctgt tggtgattgt gatcatctca cttattgtcc tggttgtcat ttggaaacag
     5101 aaaccgaggt atgaaattcg ctggagggtc attgaatcaa tcagcccgga tggacatgaa
     5161 tatatttatg tggacccgat gcagctgcct tatgactcaa gatgggagtt tccaagagat
     5221 ggactagtgc ttggtcgggt cttggggtct ggagcgtttg ggaaggtggt tgaaggaaca
     5281 gcctatggat taagccggtc ccaacctgtc atgaaagttg cagtgaagat gctaaaaccc
     5341 acggccagat ccagtgaaaa acaagctctc atgtctgaac tgaagataat gactcacctg
     5401 gggccacatt tgaacattgt aaacttgctg ggagcctgca ccaagtcagg ccccatttac
     5461 atcatcacag agtattgctt ctatggagat ttggtcaact atttgcataa gaatagggat
     5521 agcttcctga gccaccaccc agagaagcca aagaaagagc tggatatctt tggattgaac
     5581 cctgctgatg aaagcacacg gagctatgtt attttatctt ttgaaaacaa tggtgactac
     5641 atggacatga agcaggctga tactacacag tatgtcccca tgctagaaag gaaagaggtt
     5701 tctaaatatt ccgacatcca gagatcactc tatgatcgtc cagcctcata taagaagaaa
     5761 tctatgttag actcagaagt caaaaacctc ctttcagatg ataactcaga aggccttact
     5821 ttattggatt tgttgagctt cacctatcaa gttgcccgag gaatggagtt tttggcttca
     5881 aaaaattgtg tccaccgtga tctggctgct cgcaacgtcc tcctggcaca aggaaaaatt
     5941 gtgaagatct gtgactttgg cctggccaga gtcatcatgc atgattcgaa ctatgtgtcg
     6001 aaaggcagta cctttctgcc cgtgaagtgg atggctcctg agagcatctt tgacaacctc
     6061 tacaccacac tgagtgatgt ctggtcttat ggcattctgc tctgggagat cttttccctt
     6121 ggtggcaccc cttaccccgg catgatggtg gattctactt tctacaataa gatcaagagt
     6181 gggtaccgga tggccaagcc tgaccacgct accagtgaag tctacgagat catggtgaaa
     6241 tgctggaaca gtgagccgga gaagagaccc tccttttacc acctgagtga gattgtggag
     6301 aatctgctgc ctggacaata taaaaagagt tatgaaaaaa ttcacctgga cttcctgaag
     6361 agtgaccatc ctgctgtggc acgcatgcgt gtggactcag acaatgcata cattggtgtc
     6421 acctacaaaa acgaggaaga caagctgaag gactgggagg gtggtctgga tgagcagaga
     6481 ctgagcgctg acagtggcta catcattcct ctgcctgaca ttgaccctgt ccctgaggag
     6541 gaggacctgg gcaagaggaa cagacacagc tcgcagacct ctgaagagag tgccattgag
     6601 acgggttcca gcagttccac cttcatcaag agagaggacg agaccattga agacatcgac
     6661 atgatggacg acatcggcat agactcttca gacctggtgg aagacagctt cctgtagaac
     6721 ccagctttct tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gagtctagag
     6781 ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt
     6841 accggttagt aatgagatcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     6901 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     6961 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     7021 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     7081 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     7141 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     7201 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     7261 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     7321 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     7381 ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg ccacacatat
     7441 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     7501 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     7561 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     7621 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     7681 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     7741 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     7801 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     7861 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     7921 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     7981 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     8041 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     8101 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaat
     8161 cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     8221 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     8281 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     8341 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     8401 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     8461 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     8521 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     8581 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     8641 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     8701 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     8761 ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     8821 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     8881 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     8941 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     9001 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     9061 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     9121 aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat tcttgaagac
     9181 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     9241 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     9301 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     9361 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     9421 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     9481 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     9541 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     9601 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     9661 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9721 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9781 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9841 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9901 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9961 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    10021 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    10081 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    10141 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    10201 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    10261 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    10321 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    10381 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    10441 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    10501 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    10561 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    10621 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    10681 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    10741 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    10801 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10861 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10921 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10981 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    11041 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    11101 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    11161 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    11221 ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag aggccgaggc
    11281 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
    11341 ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    11401 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    11461 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    11521 acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    11581 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
    11641 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
    11701 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    11761 tatgtactac aaacttagta gt
//
