LOCUS       sequence-245657-        9551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Ayalon G, Hostettler JD, Hoffman J, Kizhatil K, Davis JQ, Bennett V
  TITLE     Ankyrin-B interactions with spectrin and dynactin-4 are required for
            dystrophin-based protection of skeletal muscle from exercise injury.
  JOURNAL   J Biol Chem. 2011 Mar 4. 286(9):7370-8.
  PUBMED    21186323
REFERENCE   2  (bases 1 to 9551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2011 Mar 4. 286(9):7370-8."
COMMENT     Sequence Label: AnkyrinB-2XHA
FEATURES             Location/Qualifiers
     source          1..9551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             6101..6127
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6128..6154
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6279..6400
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6316..6335)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6370..6389
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6407..6862)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6544..6565)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6756..6775
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6889..6993
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6995..7352
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6995..7186
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7018..7038)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7203..7338
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7387..8181
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7387..8181
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7441..7460)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8051..8070
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8369..8388)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8413..8460
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8789..9377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9278..9297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggactcag atctcgagct caagcttcga attcatgatg aacgaagatg cagctcagaa
      601 aagcgacagt ggagagaagt tcaacggcag tagtcagagg agaaaaagac ccaagaagtc
      661 tgacagcaat gcaagcttcc tccgtgctgc cagagcaggc aacctggaca aagttgtgga
      721 atatctgaag gggggcatag acatcaatac ctgcaatcag aatggactca acgctctcca
      781 tctggctgcc aaggaaggcc acgtggggct ggtgcaggag ctgctgggaa gagggtcctc
      841 tgtggattct gccactaaga agggaaatac cgctcttcac attgcatctt tggctggaca
      901 agcagaagtt gtcaaagttc ttgttaagga aggagccaat attaatgcac agtctcagaa
      961 tggctttact cctttataca tggctgccca agagaatcac attgatgttg taaaatattt
     1021 gctggaaaat ggagctaatc agagcactgc tacagaggat ggctttactc ctctagctgt
     1081 ggcactccag caaggacaca accaggcggt ggccatcctc ttggagaatg acaccaaagg
     1141 gaaagtgagg ctgccagctc tgcatattgc cgctaggaaa gacgacacca aatctgccgc
     1201 acttctgctt cagaatgacc acaatgctga cgtacaatcc aagatgatgg tgaataggac
     1261 aactgagagt ggttttaccc ctttgcacat agctgcacat tacggaaatg tcaacgtggc
     1321 aactcttctt ctaaaccggg gagctgctgt ggacttcaca gccaggaatg gaatcactcc
     1381 tctgcatgtg gcttccaaaa gaggaaatac aaacatggtg aagctcttac tggatcgagg
     1441 cggtcagatc gatgccaaaa ctagggatgg gttgacgcca cttcactgtg ctgcacgaag
     1501 tgggcatgac caagtggtgg aacttctgtt ggaacggggt gcccccttgc tggcaaggac
     1561 taagaatggg ctgtctccac tacacatggc tgcccaggga gaccacgtgg aatgtgtgaa
     1621 gcacctgtta cagcacaagg cacctgttga tgatgtcacc ctagactacc tgacagccct
     1681 ccacgttgct gcgcactgtg gccactaccg tgtaaccaaa ctccttttag acaagagagc
     1741 caatccgaac gccagagccc tgaatggttt tactccactg cacattgcct gcaagaaaaa
     1801 ccgcatcaaa gtcatggaac tgctggtgaa atatggggct tcaatccaag ctataacaga
     1861 gtctggcctc acaccaatac atgtggctgc cttcatgggc cacttgaaca ttgtcctcct
     1921 tctgctgcag aacggagcct ctccagatgt cactaacatt cgtggtgaga cggcactaca
     1981 catggcagcc cgagccgggc aggtggaagt ggtccgatgc ctcctgagaa atggtgccct
     2041 tgttgatgcc agagccaggg aggaacagac acctttacat attgcctccc gcctgggtaa
     2101 gacagaaatt gtccagctgc ttctacaaca tatggctcat ccagatgcgg ccactacaaa
     2161 tgggtacaca ccactgcaca tctctgcccg ggagggccag gtggatgtgg catcagtcct
     2221 attggaagca ggagcagccc actccttagc taccaagaag ggttttactc ccctgcatgt
     2281 agcagccaag tatggaagcc tggatgtggc aaaacttctc ttgcaacgcc gtgctgccgc
     2341 agattctgca gggaagaacg gccttacccc gctccatgtt gctgctcatt atgacaacca
     2401 gaaggtggcg ctgctgttac tggagaaggg tgcttcccct catgccactg ccaagaatgg
     2461 ctatactccg ttacatattg ctgccaagaa gaatcaaatg cagatagctt ccacactcct
     2521 gaactatgga gcagagacaa acattgtgac aaagcaagga gtaactccac tccatctggc
     2581 ctcgcaggag gggcacacag atatggttac cttgcttctg gataagggag ccaatatcca
     2641 catgtcaact aagagtggac tcacatcctt acaccttgca gcccaggaag ataaagtgaa
     2701 tgttgctgat attctcacca agcatggagc tgatcaggat gctcatacaa agcttggtta
     2761 cacaccttta attgtggcct gtcactatgg aaatgtgaaa atggtcaact ttcttctgaa
     2821 gcagggagca aatgttaacg caaaaaccaa gaacggctac acgcctttgc accaggccgc
     2881 tcagcagggt cacacgcaca tcatcaacgt cctgctccag catggggcca agcccaacgc
     2941 caccactgcg aatggcaaca ctgccttggc gattgctaag cgtctgggct acatctccgt
     3001 ggtcgacacc ctgaaggttg tgactgagga ggtcaccacc accaccacaa ctattacaga
     3061 aaaacacaaa ctaaatgtac ctgagacgat gactgaggtt cttgatgttt ctgatgaaga
     3121 gggtgatgac acaatgactg gtgatggggg agaatacctt aggcctgagg acctaaaaga
     3181 actgggtgat gactcactac ccagcagtca gttcctggat ggtatgaatt acctgcgata
     3241 cagcttggag ggaggacgat ctgacagcct tcgatccttc agttccgaca ggtctcacac
     3301 tctgagccat gcctcctacc tgagggacag tgccgtgatg gatgactcag ttgtgattcc
     3361 cagtcaccag gtgtcaactc tagccaagga ggcagaaaga aactcttatc gcctaagctg
     3421 gggcactgag aacttagaca acgtggctct ttcttctagt cctattcatt caggtttcct
     3481 ggttagtttt atggtggatg cccgaggtgg tgctatgcga ggatgcagac acaatgggct
     3541 ccgaatcatt attccacctc ggaaatgtac tgctccaacg cgagtcacct gccgactggt
     3601 caagcgccac agactggcaa caatgcctcc aatggtggaa ggagaaggcc tggccagtcg
     3661 cctgatcgaa gttggacctt ctggtgctca gttccttggg cctgtgatcg tggagatccc
     3721 tcactttgcg gcccttcgag gaaaggaaag ggaactggtg gtcctgcgca gtgagaatgg
     3781 ggacagctgg aaagagcatt tctgtgacta cactgaagat gaattgaatg aaattcttaa
     3841 cggcatggat gaagtactgg atagcccaga agacctagaa aagaaacgaa tctgccgcat
     3901 catcacccga gacttcccac agtactttgc agtggtgtct cgtatcaaac aggacagcaa
     3961 tctgattggc ccagaaggag gtgtactgag cagcacagtg gtgccccagg tgcaggccgt
     4021 cttcccagag ggggcactca ccaagcggat ccgtgtaggc ctgcaggctc aacctatgca
     4081 cagtgagctg gttaagaaga tcctaggcaa caaagctacc ttcagcccta tagtcacttt
     4141 ggaacctaga agaagaaaat tccacaaacc aattaccatg accattcctg tccccaaagc
     4201 ttcaagtgat gtcatgttga atggttttgg gggagatgca ccaaccttaa gattactatg
     4261 cagcataaca ggtggaacca cccctgccca gtgggaagat attacaggaa ctacgccatt
     4321 aacatttgtc aatgaatgtg tttcctttac aacaaacgtg tctgccaggt tctggctgat
     4381 agattgtcga cagatccagg aatccgttac ttttgcatca caagtataca gagaaattat
     4441 ctgcgtacct tatatggcca aatttgtagt gtttgccaaa tcacatgacc ccattgaagc
     4501 caggttgagg tgtttctgca tgactgatga taaagtggat aagacccttg aacaacaaga
     4561 aaattttgct gaggtggcca gaagcaggga tgtggaggtg ttagaaggaa aacccatcta
     4621 cgttgattgt ttcggcaact tggtaccatt aactaaaagt ggccagcatc atatattcag
     4681 tttttttgcc ttcaaagaaa atagacttcc tctatttgtc aaggtacgcg atacgactca
     4741 ggaaccttgc ggacgactat catttatgaa ggagccaaaa tccacgagag gcctggtgca
     4801 tcaagctatt tgcaacttaa acatcacttt gccgatttat acaaaggaat cagagtcaga
     4861 tcaagaacag gaggaagaga tcgatatgac atcagaaaaa aatccacagg atgagcagga
     4921 acggatcgag gaaaggctgg cttatattgc tgatcacctt ggcttcagct ggacagaatt
     4981 agcaagagaa ctggatttca ctgaggagca aattcatcaa attcgaattg aaaatcccaa
     5041 ctctcttcaa gaccagagtc atgcactgtt gaagtactgg ctagagaggg atgggaaaca
     5101 tgctacagat accaacctcg ttgaatgtct caccaagatc aaccgaatgg atattgttca
     5161 tctcatggag accaacacag aacctctcca ggagcgcatc agtcatagtt atgcagaaat
     5221 tgaacagacc attacactgg atcatagtga agggttctcg gtacttcaag aggagttatg
     5281 cactgcacag cacaagcaga aagaggagca agctgtttct aaagaaagtg agacctgcga
     5341 tcaccctcct atcgtctcag aggaagacat ttctgttggt tattccactt ttcaggatgg
     5401 cgtccccaaa actgaggggg acagctcatc aacagcactc tttccccaaa ctcacaagga
     5461 gcaagttcaa caggatttct cagggaaaat gcaagacctg cctgaagagt catctctgga
     5521 atatcagcag gaatattttg tgacaactcc aggaacagaa acatcagaga ctcagaaggc
     5581 tatgatagta cccagctctc ccagcaagac acctgaggaa gtcagcaccc ctgcagagga
     5641 ggagaagctg tacctccaga ccccaacatc cagcgagcgg ggaggctctc ccatcataca
     5701 agaacccgaa gagccctcag agcacagaga ggagagctct ccgcggaaaa ccagcctcgt
     5761 aatagtggag tctgccgata accagcctga gacctgtgaa agactcgatg aagatgcagc
     5821 ttttgaaaag ggagacgata tgcctgaaat acccccagaa acagtcacag aagaagaata
     5881 cattgatgag catggacaca ccgtggtaaa gaaggttact aggaaaatca ttaggcggta
     5941 tgtatcctct gaaggcacag agaaagaaga gattatggtg cagggaatgc cacaggaacc
     6001 tgtcaacatc gaggaagggg atggctattc caaagttata aagcgtgttg tattgaagag
     6061 tgacaccgag cagtcagagg acaacaatga gggtttaaac tacccatacg atgttccaga
     6121 ttacgcttac ccatacgatg ttccagatta cgcttgagcg gccgcgactc tagatcataa
     6181 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     6241 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     6301 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6361 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt
     6421 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     6481 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     6541 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     6601 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     6661 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     6721 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     6781 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     6841 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     6901 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6961 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga
     7021 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7081 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     7141 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     7201 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     7261 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     7321 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg
     7381 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     7441 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     7501 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     7561 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     7621 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     7681 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     7741 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     7801 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     7861 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg
     7921 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     7981 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     8041 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     8101 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     8161 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     8221 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     8281 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     8341 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa
     8401 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc
     8461 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt
     8521 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag
     8581 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat
     8641 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     8701 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     8761 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     8821 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     8881 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     8941 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     9001 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     9061 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     9121 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     9181 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     9241 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     9301 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     9361 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     9421 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     9481 ccgccatgca ttagttatta atagtaatca attacggggt cattagttca tagcccatat
     9541 atggagttcc g
//
