LOCUS       sequence-24566-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Harvey CD, Yasuda R, Zhong H, Svoboda K
  TITLE     The spread of Ras activity triggered by activation of a single 
            dendritic spine.
  JOURNAL   Science. 2008 Jul 4. 321(5885):136-40.
  PUBMED    18556515
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jul 4. 321(5885):136-40."
COMMENT     Sequence Label: paGFP-HRas G12V
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(301..552)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSHQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(343..364)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     692..711
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctctgncggt ggagtcctca aagacttggt gttgttgatg gcaaacacac acaggaagcc
       61 ctccccggtg cgcatgtact ggtcccgcat ggcgctgtac tcctcctggc cggcggtatc
      121 caggatgtcc aacaggcacg tctccccatc aatgaccacc tgcttccggt aggaatcctc
      181 tatagtgggg tcgtattcgt ccacaaaatg gttctggatc agctggatgg tcagcgcact
      241 cttgcccaca ccgacggcgc ccaccaccac cagcttatat tccgtcatct cgagtccgga
      301 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
      361 catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactggtggc tcaggtagtg
      421 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
      481 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttggcct tgatgccgtt
      541 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
      601 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
      661 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
      721 gatggtgctg tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
      781 catgtggtcg gggtagcggc tgaacactg
//
