LOCUS       sequence-24567-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Harvey CD, Yasuda R, Zhong H, Svoboda K
  TITLE     The spread of Ras activity triggered by activation of a single 
            dendritic spine.
  JOURNAL   Science. 2008 Jul 4. 321(5885):136-40.
  PUBMED    18556515
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jul 4. 321(5885):136-40."
COMMENT     Sequence Label: MARCKS(1-40)-paGFP
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        247..265
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     247..264
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     278..297
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     complement(545..566)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggnnattagc agagctcgtt agtgaaccgt cagatcgcct ggagacgcca tccacgctgn
       61 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat
      121 tggaacgcgg attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc
      181 cacccccttg gcttcgttag aacgcggcta caattaatac ataaccttat gtatcataca
      241 catacgattt aggtgacact atagaataac atccactttg cctttctctc cacaggtgtc
      301 cactcccagg tccaactgca cctcggttct atcgattgaa ttcccgggct cgagcagcat
      361 gggttgctgt ttctccaaga ccgcagcgaa gggagaagcc gccgcggaga ggcctgggga
      421 ggcggctgtg gcctcgtcgc cttccaaagc gaacggacag gagaatggcc acgtgaaggt
      481 aggatcctct agagtcgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
      541 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
      601 cgagggcgat gccacctacg gcaagctgac cctgaagttc atngtnacca ccgggcaagc
      661 tgcccgtgcc ctggcngacc ctcgtgacca ccttcaagct acgggggtgc agtgnttcag
      721 cccttccccn naaccacatg aagcagcacg acttcttcaa gtccgcatgc cnnaagntac
      781 ntcnagancg accatntttn aagaaaangg nattaaaaac ccncccaggt nattngaggn
      841 naccctgtna cnaatc
//
