LOCUS       sequence-24568-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Harvey CD, Yasuda R, Zhong H, Svoboda K
  TITLE     The spread of Ras activity triggered by activation of a single 
            dendritic spine.
  JOURNAL   Science. 2008 Jul 4. 321(5885):136-40.
  PUBMED    18556515
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jul 4. 321(5885):136-40."
COMMENT     Sequence Label: mEGFP
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        248..266
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     248..265
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     279..298
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      354..363
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(405..426)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cnngggnatt agcagagctg ttagtgaacc gtcagatcgc ctggagacgc catccacgct
       61 gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggccgg gaacggtgca
      121 ttggaacgcg gattccccgt gccaagagtg acgtaagtac cgcctataga gtctataggc
      181 ccaccccctt ggcttcgtta gaacgcggct acaattaata cataacctta tgtatcatac
      241 acatacgatt taggtgacac tatagaataa catccacttt gcctttctct ccacaggtgt
      301 ccactcccag gtccaactgc acctcggttc tatcgattga attcgtaccg gtcgccacca
      361 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
      421 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
      481 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
      541 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
      601 agcacgactt cttcaagtcc gcctgccccn aagggtactc tcagggagcg naccatcttc
      661 ttcanggacn acggcaacta caagacccgc gccgaggtga agttcgaggn cnaaccctgn
      721 tgaacnctct nnntgtanng gnttncnttt nngggaggac ggcaacatcc tggggcacaa
      781 gctggataca ctacacagca cacgtctatt natggcgaca ncaaanangn ctcagggaat
      841 tcanaccncn aantcaagaa
//
