LOCUS       sequence-245691-        1129 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Drosophila melanogaster Cofilin (Twinstar) in bacterial 
            cell culture.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Figard L, Zheng L, Biel N, Xue Z, Seede H, Coleman S, Golding I, 
            Sokac AM
  TITLE     Cofilin-Mediated Actin Stress Response Is Maladaptive in 
            Heat-Stressed Embryos.
  JOURNAL   Cell Rep. 2019 Mar 26;26(13):3493-3501.e4. doi: 
            10.1016/j.celrep.2019.02.092.
  PUBMED    30917306
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.02"; journalName: "Cell Rep"; date: 
            "2019-03-26- 26"; volume: "26"; issue: "13"; pages: "3493-3501.e4"
COMMENT     Sequence Label: pET45b-tsr(B/X) (6X-His-Dm-Cofilin)
FEATURES             Location/Qualifiers
     source          1..1129
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..79
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             98..112
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             581..625
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(663..681)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      677..724
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(848..867)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggggggcaca ttctctctat aataattttg tttaacttta ataaggagat ataccatggc
       61 acatcaccac caccatcacg tgggtaccgg ttcgaatgat gacgacgaca agagtccgga
      121 tcccgcttct ggtgtaactg tgtctgatgt ctgcaagact acatacgagg aaataaagaa
      181 ggacaaaaag catcgctatg tcatctttta cattcgcgat gaaaagcaga ttgatgtgga
      241 gactgtggca gatcgcaacg ccgagtacga ccagtttcta gaagatatcc agaaatgcgg
      301 acctggagag tgccggtacg gactgttcga tttcgagtac atgcaccagt gccaaggcac
      361 ctctgagagt tcaaagaagc aaaagctgtt ccttatgtcg tggtgtcccg atactgccaa
      421 ggtcaagaag aagatgttgt actccagctc cttcgatgct ctcaagaagt cgctcgtggg
      481 cgtccaaaag tatatacaag ccactgatct ttccgaagcc tcccgggaag ccgtcgagga
      541 gaaactccgg gccaccgacc gccaataact cgagtctggt aaagaaaccg ctgctgcgaa
      601 atttgaacgc cagcacatgg actcgtctac tagcgcagct taattaacct aggctgctgc
      661 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
      721 tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca
      781 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      841 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
      901 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
      961 cccaaaaaac ttgattaggg tgatgggttc acgtagtggg ccatcgccct gatagacgtt
     1021 tttcgccctt gacgttggag tcacgtcttt atagtggact ctgatccaac tgacaacact
     1081 cagcctatct cgggtcttat tcctttgacc ttattaagga tttttgcga
//
