LOCUS       sequence-245693-       12023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lineage specific over expression of a mutated TFEB in lung alveolar 
            epithelial cells, where TFEB is missing the first 30 amino acids of 
            the sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12023)
  TITLE     Kotton lab unpublished
REFERENCE   2  (bases 1 to 12023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHAGE-hSPC-TFEB(delta30)eGFP-W
FEATURES             Location/Qualifiers
     source          1..12023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2396..2418
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2410..2427
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2411..2427
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      6303..6312
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      7680..7689
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7686..7688,7689..7691,7692..8405)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7686..7688,7689..7691,7692..8402)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7731..7752)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7992..8011)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8339..8360
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8413..9001
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8466..8486)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8884..8895)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9076..9309
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9428..9446)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9514..9618
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9619..9687,9688..10479)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9837..9856)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10650..11238
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11139..11158
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11392..11409
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11649..11670
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11685..11690,11691..11708,11709..11715)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11723..11739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11728..11750
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11747..11763
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11747..11763
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11813..11832
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtatg
     2221 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     2281 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     2341 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     2401 tcacgacgtt gtaaaacgac ggccagtgcc aagcttgaag actgctgctc tctaccacgt
     2461 tagctcccct gtggcggagg atgactgtcc taagagctca taggcacggg gcaaggggag
     2521 cctggctgtc agctgcctgg gcttctagtc ttgtgctttt tgcacaccag tccagggaac
     2581 gaagaccact ggctttaaga tgcttcccca gctgtcccca gactctgcca gcaggggatt
     2641 ctctggtctg agcttaagtt gtgttctccc agccagggat gcccctgccc tttgatgtct
     2701 ccttgctgcc acacatttag ccgccctccc catgccagct tggggggagg gaagcagtgg
     2761 gggtagggag gtggctgggg cagctgggca actgtcccca cccgtccctg gcacggctct
     2821 gcccagtaca caaagagcaa agtgaatctt gtccccaccc ctgcagctga ggggctggag
     2881 gaggaaacgg ggaggcccac acagaagggg tggccaccgt ggggctgtcc atcactcagg
     2941 gctctcagag ggagtcaacc cagaaacaga caaagagggt gagtctgggc tgtgttctta
     3001 gctagtgaga ggtcccctag aggatgaagt agatgatgct aatgaggatg actggatgtc
     3061 acacccatga tgctattagg tcctcataat agcatagtga ggtggacagc tagtacctga
     3121 cccatctcac agatgaacat actaatgcct aacaaagcag aacgactcac gctgggtccc
     3181 agagctggcc agtggaagca ctgagacctc cacatactga aggcatggac tattgaccgc
     3241 tgttggtatt ggtctcatca ttgactatca ttaagtgttg gctgtttgca tgcttcctgc
     3301 ccagtggcag gttcaaagaa gcccgcagga agcgtgctcc tttctttccc agggcccgca
     3361 attgggctgg aagatagagc aacaaaaagc gcccatgtaa ctcatgggaa cattcatgtg
     3421 tgctgaatgg caggtgaagg tgccacagag aggctgagga tttcagaggg caccatgaac
     3481 tggagtgagg tcgcagagca ggtgccattg gctcttggcc tgtttgggtg ggtggcattc
     3541 agagaggtgg aaggtcagat gcactgttca cgcctgtaat ctcagcactt tggaaggcca
     3601 aggtaggagg atcacacgag gccaggagat caacgctgca gtgagctatg aagctgtgat
     3661 tgcaccactg cactgcagct tgggtaacag agtgagaccc tgtctctaaa taattaaata
     3721 aataaaataa aaataaaacc ggagaagtgg agagggattg gaggtgggct ttcacagagg
     3781 gagaaacggc ttaagtacag gccaaaaagt gagaaggctg cagacagggc tggtaggggg
     3841 agggggaaat ttggcacacc cagctaaagg tccttctgtg tctccttctc caaggaaccc
     3901 aagaccttca cttggttggt gtgagcactc caggaggcag gcaccctccc tcagccctca
     3961 agcaagcaaa aatgggttta aaaaaagaag gagaagaagc agcagcagca gccgccacag
     4021 agcttgtgac agctacagcc taagggcaac aggcagggga gaccaaggac cagaaagagc
     4081 agaggctttt tcaaagaaag agatccctct cccagcaccc agcgatggcg gcaaacccca
     4141 cccacagtgc ctgctaagaa caagtcccac gtgagaacaa catggccccc cgagatgccc
     4201 acagggaccc cgagatgcct gcagtgcttg gctctcccgc tggccagctg cccacctggc
     4261 tcaggcccag tactcgtgag tcagccgatc aatcccaatg ttgccaggat gatgggggcg
     4321 ggagtaaggg ccctggggga gggcaggggt gggcactgca ggcgagctgt ctcccacatc
     4381 tggcacctgc acacagctga ggccgagctg agaggatgct tctgtgggct ccccctcctc
     4441 ccggcacccc tcccctcctt tcactgtcct aggacactct ctggctgctg gagtcttagg
     4501 caaatattta aaggggcagc aagggggtga ggagggtggt gggagcaaac acttccctcc
     4561 ctttcttcct ccctgcgctt ctcaggggct ctcagttcag atgccatgct gttatgcaac
     4621 cttggggctg aaggccctcc agatggagag ggggacaggg gaacctgcca gctcatgacc
     4681 gaagggcagg gcccaggtgg gagggggctg gggcagggga caggaactgg ggtggcatgt
     4741 taaaggacag gaggctggtt gggcatggtg gctcacacct gtaatcctag cactttagga
     4801 ggccgagatc acttgagccc aggagttcaa gaccagcctg ggcaacatgg tgaaaactca
     4861 tctctataaa acaagcaaaa attagctggg cacaatggca tgcactggta gtcccagcta
     4921 cttgggatgc tgaggtgtga ggatcaccgg agtccaggag gtcaacgctg cagtgagcag
     4981 tgatctcgct actgcatgcc agcctgggtg ataaagtgag accctgtctc acaacaaaac
     5041 aaaacaaaac aaaacaaaag gataggaggt taagggagcg agcccaggcc tggactctgc
     5101 cacagtcacc tgagtttaga ggtgaaggga ctttagagac cacctggccc aaggagtgaa
     5161 agggaacctg agagaaaggg tgagccagcc caaagtcatt ggcagatttg acctcgtaaa
     5221 tacatagaga tggctttggg aaggcactag gaaagacaga gaaaagagaa ggagacagtc
     5281 ctcaaagctg atcgtatttg ggtgagtatt attctcaggg caaatttagg atcagaggat
     5341 gcagaaaggg gagtctagag gggtagagtg tagaccacag ggtgagtgag ctgattcgag
     5401 gatggggaga ctgggagccc accagtgacc agagccagcc ctgttcaggg ctgtccgggc
     5461 agaagaaagc agtgtcagac ctggaatctg ccatcagcac agcctgcaat tgacagacaa
     5521 gcccagagca aagaaggaag cactgcacat gagtaagagc ttgccaccag tggggacaga
     5581 gtttccagaa ttaggaaaat aatcactggg ggcaagtttg aggttggtac cagatatgtg
     5641 ggaggaggca aggtaaggga aagagtactt gaagttggaa ctggtccttg cagggaaatg
     5701 cacatttatg aaaccccgaa aactgatgtc aaagcacctc ctgccttggg cagagtcctc
     5761 tcagagtcta caggtgctgc ctccagaacc ctcttcctgg agcgcatccc tatgtatcta
     5821 gaaattctgc tgggaaatat gatggtcaga cccttggcca cctgaaaggt tcagggtggt
     5881 agaagaaaaa ggaaagccac agggcagcag gggcaggtgc cagcaaggaa ggcaggcacg
     5941 ccaggaagac acccatggtg agaagtgcag atggcccgag ggcaagtttg ctcaactcac
     6001 ccaggtttgc tcttgctggg gccaagagga ctcatgtgcc agggccaagg gcccttgggg
     6061 gctctcacag ggggcttatc tgggcttcgg ttctggaggg ccaggaacaa acaggcttca
     6121 aagccaaggg cttggctggc acacaggggg cttggtcctt cacctctgtc ccctctccct
     6181 acggacacat ataagaccct ggtcacacct gggagaggag gagaggagag catagcacct
     6241 gcaggtcgac tgaaccgtca gatccgctag cgctaccgga ctcagatctc gagctcaagc
     6301 ttgccaccat ggattacatg cagcagcagc agcagcagca acagcagcag ctcggagggc
     6361 cgcccacccc ggccatcaat acccccgtcc acttccagtc gccaccacct gtgcctgggg
     6421 aggtgttgaa ggtgcagtcc tacctggaga atcccacatc ctaccatctg cagcagtcgc
     6481 agcatcagaa ggtgcgggag tacctgtccg agacctatgg gaacaagttt gctgcccaca
     6541 tcagcccagc ccagggctct ccgaaacccc caccagccgc ctccccaggg gtgcgagctg
     6601 gacacgtgct gtcctcctcc gctggcaaca gtgctcccaa tagccccatg gccatgctgc
     6661 acattggctc caaccctgag agggagttgg atgatgtcat tgacaacatt atgcgtctgg
     6721 acgatgtcct tggctacatc aatcctgaaa tgcagatgcc caacacgcta cccctgtcca
     6781 gcagccacct gaatgtgtac agcagcgacc cccaggtcac agcctccctg gtgggcgtca
     6841 ccagcagctc ctgccctgcg gacctgaccc agaagcgaga gctcacagat gctgagagca
     6901 gggccctggc caaggagcgg cagaagaaag acaatcacaa cttaattgaa aggagacgaa
     6961 ggttcaacat caatgaccgc atcaaggagt tgggaatgct gatccccaag gccaatgacc
     7021 tggacgtgcg ctggaacaag ggcaccatcc tcaaggcctc tgtggattac atccggagga
     7081 tgcagaagga cctgcaaaag tccagggagc tggagaacca ctctcgccgc ctggagatga
     7141 ccaacaagca gctctggctc cgtatccagg agctggagat gcaggctcga gtgcacggcc
     7201 tccctaccac ctccccgtcc ggcatgaaca tggctgagct ggcccagcag gtggtgaagc
     7261 aggagctgcc tagcgaagag ggcccagggg aggccctgat gctgggggct gaggtccctg
     7321 accctgagcc actgccagct ctgcccccgc aagccccgct gcccctgccc acccagccac
     7381 cgtccccatt ccatcacctg gacttcagcc acagcctgag ctttgggggc agggaggacg
     7441 agggtccccc gggctacccc gaacccctgg cgccggggca tggctcccca ttccccagcc
     7501 tgtccaagaa ggatctggac ctcatgctcc tggacgactc actgctaccg ctggcctctg
     7561 atccacttct gtccaccatg tcccccgagg cctccaaggc cagcagccgc cggagcagct
     7621 tcagcatgga ggagggcgat gtgctgaagg taccgcgggc ccgggatcca ccggtcgcgg
     7681 ccgccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     7741 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     7801 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     7861 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     7921 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     7981 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     8041 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     8101 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     8161 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     8221 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     8281 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     8341 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     8401 agtaaggatc ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     8461 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     8521 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     8581 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     8641 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     8701 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     8761 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     8821 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     8881 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     8941 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     9001 cctgagatcc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     9061 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     9121 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     9181 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     9241 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     9301 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     9361 gaatatcaga gagtgagagg cccgggttaa ttaaggaaag ggctagatca ttcttgaaga
     9421 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     9481 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     9541 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     9601 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     9661 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     9721 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     9781 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     9841 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac
     9901 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     9961 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
    10021 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
    10081 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
    10141 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
    10201 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
    10261 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
    10321 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
    10381 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
    10441 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
    10501 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
    10561 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
    10621 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    10681 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    10741 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
    10801 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    10861 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    10921 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    10981 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    11041 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    11101 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    11161 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    11221 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    11281 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    11341 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
    11401 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    11461 attcattaat gcagcaagct catggctgac taattttttt tatttatgca gaggccgagg
    11521 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
    11581 tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
    11641 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
    11701 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
    11761 gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    11821 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
    11881 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
    11941 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
    12001 atatgtacta caaacttagt agt
//
