LOCUS       sequence-245698-       12785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR-Cas9 based efficient genome editing in S. aureus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12785)
  AUTHORS   Liu Q, Jiang Y, Shao L, Yang P, Sun B, Yang S, Chen D
  TITLE     CRISPR/Cas9-based efficient genome editing in Staphylococcus aureus.
  JOURNAL   Acta Biochim Biophys Sin (Shanghai). 2017 Sep 1;49(9):764-770. doi: 
            10.1093/abbs/gmx074.
  PUBMED    28910979
REFERENCE   2  (bases 1 to 12785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Acta Biochim Biophys Sin (Shanghai)"; date: "2017-09-1- 1"; volume:
            "49"; issue: "9"; pages: "764-770"
COMMENT     Sequence Label: pLQ-KO-tgt50
FEATURES             Location/Qualifiers
     source          1..12785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1720..1763)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1722..1803
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1822..1849
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      1929..1963
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     protein_bind    2262..2278
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_RNA        2301..2376
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    3955..3973
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             3998..5716
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     primer_bind     complement(8147..8163)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8147..8163)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8160..8182)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8171..8187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8195..8201,8202..8219,8220..8225))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8240..8261
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10385..10402)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10556..11144)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10636..10655)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11315..12106,12107..12175))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11938..11957
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12176..12280)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12348..12366
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(12404..12426)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     12526..12545
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12739..12761
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12753..12770
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12754..12770
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctgcagaacg gattgttgat gattacgaaa atattaagag cacagactat tacacagaaa
       61 atcaagaatt aaaaaaacgt agagagagtt tgaaagaagt agtgaataca tggaaagagg
      121 ggtatcacga aaaaagtaaa gaggttaata aattaaagcg agagaatgat agtttgaatg
      181 agcagttgaa tgtatcagag aaatttcaag atagtacagt gactttatat cgtgctgcga
      241 gggcgaattt ccctgggttt gagaaagggt ttaataggct taaagagaaa ttctttaatg
      301 attccaaatt cgagcgtgtg ggacagttta tggatgttgt acaggataat gtccagaagg
      361 tcgatagaaa gcgtgagaaa cagcgtacag acgatttaga gatgtagagg tacttttatg
      421 ccgagaaaac tttttgcgtg tgacagtcct taaaatatac ttagagcgta agcgaaagta
      481 gtagcgacag ctattaactt tcggttgcaa agctctagga tttttaatgg acgcagcgca
      541 tcacacgcaa aaaggaaatt ggaataaatg cgaaatttga gatgttaatt aaagaccttt
      601 ttgaggtctt tttttcttag atttttgggg ttatttaggg gagaaaacat aggggggtac
      661 tacgacctcc cccctaggtg tccattgtcc attgtccaaa caaataaata aatattgggt
      721 ttttaatgtt aaaaggttgt tttttatgtt aaagtgaaaa aaacagatgt tgggaggtac
      781 agtgatggtt gtagatagaa aagaagagaa aaaagttgct gttactttaa gacttacaac
      841 agaagaaaat gagatattaa atagaatcaa agaaaaatat aatattagca aatcagatgc
      901 aaccggtatt ctaataaaaa aatatgcaaa ggaggaatac ggtgcatttt aaacaaaaaa
      961 agatagacag cactggcatg ctgcctatct atgactaaat tttgttaaat gtattagcac
     1021 cgttattata tcatgagcga aaatgtaata aaagaaactg aaaacaagaa aaattcaaga
     1081 ggacgtaatt ggacatttgt tttatatcca gaatcagcaa aagccgagtg gttagagtat
     1141 ttaaaagagt tacacattca atttgtagtg tctccattac atgataggga tactgataca
     1201 gaagatagga tgaaaaaaga gcattatcat attctagtga tgtatgaggg taataaatct
     1261 tatgaacaga taaaaataat tacagaagaa ttgaatgcga ctattccgca gattgcagga
     1321 agtgtgaaag gtcttgtgag atatatgctt cacatggacg atcctaataa atttaaatat
     1381 caaaaagaag atatgatagt ttatggcggt gtagatgttg atgaattatt aaagaaaaca
     1441 acaacagata gatataaatt aattaaagaa atgattgagt ttattgatga acaaggaatc
     1501 gtagaattta agagtttaat ggattatgca atgaagttta aatttgatga ttggttcccg
     1561 cttttatgtg ataactcggc gtatgttatt caagaatata taaaatcaaa tcggtataaa
     1621 tctgaccgat agattttgaa tttaagagtg tcacaagaca ctcttttttc gcaccagcga
     1681 aaactggttt aagccgactg cgcaaaagac atactcgaga taaaacgaaa ggctcagtcg
     1741 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     1801 aatccgccgc tctagctaag cagaaggcca tcctgacgga tggccttttt gcgtttctac
     1861 aaactcttgt taactctaga gctgcctgcc gcgtttcggt gatgaagatc ttcccgatga
     1921 ttaattaatt cagaacgctc ggttgccgcc gggcgttttt tatgcagcaa tggcaagaac
     1981 gttgctctag aataattcta cacagcccag tccagactat tcggcactga aattatgggt
     2041 gaagtggtca agacctcact aggcacctta aaaatagcgc accctgaaga agatttattt
     2101 gaggtagccc ttgcctacct agcttccaag aaagatatcc taacagcaca agagcggaaa
     2161 gatgttttgt tctacatcca gaacaacctc tgctaaaatt cctgaaaaat tttgcaaaaa
     2221 gttgttgact ttatctacaa ggtgtggcat aatgtgtgga attgtgagcg gataacaatt
     2281 atgtgttcgt atgttactgc gttttagagc tagaaatagc aagttaaaat aaggctagtc
     2341 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttgcggccg cgtgatctaa
     2401 tgattcatat ctacatcaaa tgctggggca tatgttttac cctcaggtac aggtaagtca
     2461 cccgcttcaa acgtcttctt aatatgcgta attgcatcta ctctaaatcc atcaatgcct
     2521 ttatcaaacc accagttcat catttcaaat acagcatctc taacttccgg attaccccaa
     2581 ttcaaatcag gttgtttttt actgaataaa tggaaataat attgctcagt attagcatca
     2641 tattcccgcg cgccaattga gctccccttt ctatgtatgt tttttactag tcatttaaaa
     2701 cgatacatta ataggtacga aaaagcaact ttttttgcgc ttaaaaccag tcataccaat
     2761 aacttaaggg taactagcct cgccggcaat agttaccctt attatcaaga taagaaagaa
     2821 aaggattttt cgctacgctc aaatccttta aaaaaacaca aaagaccaca ttttttaatg
     2881 tggtctttta ttcttcaact aaagcaccca ttagttcaac aaacgaaaat tggataaagt
     2941 gggatatttt taaaatatat atttatgtta cagtaatatt gacttttaaa aaaggattga
     3001 ttctaatgaa gaaagcagac aagtaagcct cctaaattca ctttagataa aaatttagga
     3061 ggcatatcaa atgaaattta ataaaattga tttagacaat tggaagagaa aagagatatt
     3121 taatcattat ttgaaccaac aaacgacttt tagtataacc acagaaattg atattagtgt
     3181 tttataccga aacataaaac aagaaggata taaattttac cctgcattta ttttcttagt
     3241 gacaagggtg ataaactcaa atacagcttt tagaactggt tacaatagcg acggagagtt
     3301 aggttattgg gataagttag agccacttta tacaattttt gatggtgtat ctaaaacatt
     3361 ctctggtatt tggactcctg taaagaatga cttcaaagag ttttatgatt tatacctttc
     3421 tgatgtagag aaatataatg gttcggggaa attgtttccc aaaacaccta tacctgaaaa
     3481 tgctttttct ctttctatta ttccatggac ttcatttact gggtttaact taaatatcaa
     3541 taataatagt aattaccttc tacccattat tacagcagga aaattcatta ataaaggtaa
     3601 ttcaatatat ttaccgctat ctttacaggt acatcattct gtttgtgatg gttatcatgc
     3661 aggattgttt atgaactcta ttcaggaatt gtcagatagg cctaatgact ggcttttata
     3721 atatgagata atgccgactg tactttttac agtcggtttt ctaatgtcac taacctgccc
     3781 cgttagttga agaaggtttt tatattacag ctccagatcc atatccttct ttttctgaac
     3841 cgacttctcc tttttcgctt ctttattcca attgctttat tgacgttgag cctcggaacc
     3901 gtcgacttta tttggatccc ctcgagttca tgaaaaacta aaaaaaatat tgacactcta
     3961 tcattgatag agtataatta acaaggagga ataaaaaatg gataagaaat actcaatagg
     4021 cttagatatc ggcacaaata gcgtcggatg ggcggtgatc actgatgaat ataaggttcc
     4081 gtctaaaaag ttcaaggttc tgggaaatac agaccgccac agtatcaaaa aaaatcttat
     4141 aggggctctt ttatttgaca gtggagagac agcggaagcg actcgtctca aacggacagc
     4201 tcgtagaagg tatacacgtc ggaagaatcg tatttgttat ctacaggaga ttttttcaaa
     4261 tgagatggcg aaagtagatg atagtttctt tcatcgactt gaagagtctt ttttggtgga
     4321 agaagacaag aagcatgaac gtcatcctat ttttggaaat atagtagatg aagttgctta
     4381 tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa ttggtagatt ctactgataa
     4441 agcggatttg cgcttaatct atttggcctt agcgcatatg attaagtttc gtggtcattt
     4501 tttgattgag ggagatttaa atcctgataa tagtgatgtg gacaaactat ttatccagtt
     4561 ggtacaaacc tacaatcaat tatttgaaga aaaccctatt aacgcaagtg gagtagatgc
     4621 taaagcgatt ctttctgcac gattgagtaa atcaagacga ttagaaaatc tcattgctca
     4681 gctccccggt gagaagaaaa atggcttatt tgggaatctc attgctttgt cattgggttt
     4741 gacccctaat tttaaatcaa attttgattt ggcagaagat gctaaattac agctttcaaa
     4801 agatacttac gatgatgatt tagataattt attggcgcaa attggagatc aatatgctga
     4861 tttgtttttg gcagctaaga atttatcaga tgctatttta ctttcagata tcctaagagt
     4921 aaatactgaa ataactaagg ctcccctatc agcttcaatg attaaacgct acgatgaaca
     4981 tcatcaagac ttgactcttt taaaagcttt agttcgacaa caacttccag aaaagtataa
     5041 agaaatcttt tttgatcaat caaaaaacgg atatgcaggt tatattgatg ggggagctag
     5101 ccaagaagaa ttttataaat ttatcaaacc aattttagaa aaaatggatg gtactgagga
     5161 attattggtg aaactaaatc gtgaagattt gctgcgcaag caacggacct ttgacaacgg
     5221 ctctattccc catcaaattc acttgggtga gctgcatgct attttgagaa gacaagaaga
     5281 cttttatcca tttttaaaag acaatcgtga gaagattgaa aaaatcttga cttttcgaat
     5341 tccttattat gttggtccat tggcgcgtgg caatagtcgt tttgcatgga tgactcggaa
     5401 gtctgaagaa acaattaccc catggaattt tgaagaagtt gtcgataaag gtgcttcagc
     5461 tcaatcattt attgaacgca tgacaaactt tgataaaaat cttccaaatg aaaaagtact
     5521 accaaaacat agtttgcttt atgagtattt tacggtttat aacgaattga caaaggtcaa
     5581 atatgttact gaaggaatgc gaaaaccagc atttctttca ggtgaacaga agaaagccat
     5641 tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa aagaagatta
     5701 tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca ggagttgaag atagatttaa
     5761 tgcttcatta ggtacctacc atgatttgct aaaaattatt aaagataaag attttttgga
     5821 taatgaagaa aatgaagata tcttagagga tattgtttta acattgacct tatttgaaga
     5881 tagggagatg attgaggaaa gacttaaaac atatgctcac ctctttgatg ataaggtgat
     5941 gaaacagctt aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa aattgattaa
     6001 tggtattagg gataagcaat ctggcaaaac aatattagat tttttgaaat cagatggttt
     6061 tgccaatcgc aattttatgc agctgatcca tgatgatagt ttgacattta aagaagacat
     6121 tcaaaaagca caagtgtctg gacaaggcga tagtttacat gaacatattg caaatttagc
     6181 tggtagccct gctattaaaa aaggtatttt acagactgta aaagttgttg atgaattggt
     6241 caaagtaatg gggcggcata agccagaaaa tatcgttatt gaaatggcac gtgaaaatca
     6301 gacaactcaa aagggccaga aaaattcgcg agagcgtatg aaacgaatcg aagaaggtat
     6361 caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc aattgcaaaa
     6421 tgaaaagctc tatctctatt atctccaaaa tggaagagac atgtatgtgg accaagaatt
     6481 agatattaat cgtttaagtg attatgatgt cgatcacatt gttccacaaa gtttccttaa
     6541 agacgattca atagacaata aggtcttaac gcgttctgat aaaaatcgtg gtaaatcgga
     6601 taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac tattggagac aacttctaaa
     6661 cgccaagtta atcactcaac gtaagtttga taatttaacg aaagctgaac gtggaggttt
     6721 gagtgaactt gataaagctg gttttatcaa acgccaattg gttgaaactc gccaaatcac
     6781 taagcatgtg gcacaaattt tggatagtcg catgaatact aaatacgatg aaaatgataa
     6841 acttattcga gaggttaaag tgattacctt aaaatctaaa ttagtttctg acttccgaaa
     6901 agatttccaa ttctataaag tacgtgagat taacaattac catcatgccc atgatgcgta
     6961 tctaaatgcc gtcgttggaa ctgctttgat taagaaatat ccaaaacttg aatcggagtt
     7021 tgtctatggt gattataaag tttatgatgt tcgtaaaatg attgctaagt ctgagcaaga
     7081 aataggcaaa gcaaccgcaa aatatttctt ttactctaat atcatgaact tcttcaaaac
     7141 agaaattaca cttgcaaatg gagagattcg caaacgccct ctaatcgaaa ctaatgggga
     7201 aactggagaa attgtctggg ataaagggcg agattttgcc acagtgcgca aagtattgtc
     7261 catgccccaa gtcaatattg tcaagaaaac agaagtacag acaggcggat tctccaagga
     7321 gtcaatttta ccaaaaagaa attcggacaa gcttattgct cgtaaaaaga ctgggatcca
     7381 aaaaaatatg gtggttttga tagtccaacg gtagcttatt cagtcctagt ggttgctaag
     7441 gtggaaaaag ggaaatcgaa gaagttaaaa tccgttaaag agttactagg gatcacaatt
     7501 atggaaagaa gttcctttga aaaaaatccg attgactttt tagaagctaa aggatataag
     7561 gaagttaaaa aagacttaat cattaaacta cctaaatata gtctttttga gttagaaaac
     7621 ggtcgtaaac ggatgctggc tagtgccgga gaattacaaa aaggaaatga gctggctctg
     7681 ccaagcaaat atgtgaattt tttatattta gctagtcatt atgaaaagtt gaagggtagt
     7741 ccagaagata acgaacaaaa acaattgttt gtggagcagc ataagcatta tttagatgag
     7801 attattgagc aaatcagtga attttctaag cgtgttattt tagcagatgc caatttagat
     7861 aaagttctta gtgcatataa caaacataga gacaaaccaa tacgtgaaca agcagaaaat
     7921 attattcatt tatttacgtt gacgaatctt ggagctcccg ctgcttttaa atattttgat
     7981 acaacaattg atcgtaaacg atatacgtct acaaaagaag ttttagatgc cactcttatc
     8041 catcaatcca tcactggtct ttatgaaaca cgcattgatt tgagtcagct aggaggtgac
     8101 tgaagtatat tttagatgaa gattatttct taagaattct gactatgtca tagctgtttc
     8161 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     8221 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     8281 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8341 ggagaggcgg tttgcgtatt gggcgctctt ccaaacgcct ttaaaagatc gtgatcatag
     8401 tcgtttatta gtcatggata gagaaactgg tgaaatgaaa catttacatt tcaaagatat
     8461 cattgagtat tttagacctg gtgatacatt agtgcttaac gatacgcgag taatgccagc
     8521 tagacttttt ggtttaaaag aagaaactgg tgcaaaagtt gaaatgttaa tgttaactca
     8581 aattgaaggt aatgattggg aagtcttact gaaaccagct aagcgtatta aagttggtaa
     8641 taaattgaat tttggtaatg gcaaaattat agctgaatgc ataaaagaaa tggatcaagg
     8701 tggacgcatc atgcgtttac attatgaagg tattttacaa gaaagattag atgaattagg
     8761 ggaaatgcca ctgccaccat acatcaaaga acgtttagat gatccagatc gttatcaaac
     8821 agtttacgct aaagaaagtg gttcagcggc agcaccaaca gcaggattac attttactga
     8881 tgagttatta attgaaatta aaaataaagg tgttaatatc gcatttgtta cattacatgt
     8941 tgggttaggt acgtttagac cggtgagcgt agacgatgtg aatgaccacg aaatgcatag
     9001 tgaatattat caaatgacac aagaaacagc tgatttatta aatgatacta agtccaaagg
     9061 acatcgcatt atatcagttg gtacaacttc aacacgtaca cttgaaacaa ttcgacgcga
     9121 tcatgataaa tttgttgaaa cgagtggctg gactaatata tttatttatc caggatttga
     9181 ttttaaagca attgatggcc agattactaa ttttcattta ccaaaatcaa cattagttat
     9241 gctagtatca gcgtttagta gtcgtgaaaa tgttctgaat gcttataaaa cggcagtaaa
     9301 tttagaatat agattcttta gttttggcga tgcaatgtta attatataaa aagaatgtga
     9361 ggattttgaa tgatatcgcg tttaggtatc gtgcacacac cacacggttc atttgaaaca
     9421 cctatgttta tgccagttgg tactaaagca accgttaaaa caatgagtcc agaagagtta
     9481 agacaaattg aagcaaaaat cattttgggc aacacatatc atttgtggtt acaacccgga
     9541 aatgatatta tcaaacacgc tgggggatta cataaattca tgaattggga tggtccgatt
     9601 cttacagatt caggcggttt ccaagtgttt agtttaagta atttacgtaa aattacagaa
     9661 gaaggcgtgg aatttagaca tcatactaat gggtctaaat tatttttgag tcctgagaaa
     9721 tcaatgcaaa ttcaaaatga tttaggatct gatattatga tggcatttga tgaatgtcca
     9781 ccgatgcctg ctgaatatga ttatgtaaaa aaatctattg aacgtacaac acgttgggcg
     9841 aaaagatgtc tagatgcaca ccaaagacct gaagatcaag cattgttcgg cattatacaa
     9901 ggtggcgaat atgaagattt aagagaacaa agtgcaaagg atttagtaga attagatttt
     9961 cctggttatg caatcggcgg tttgtcagtt ggtgaaccta aaccggttat gtataaaatg
    10021 gttgaacata cagagcagtt tatgcctaaa gataaaccaa gatatttaat gggtgtagga
    10081 tctccagatg cgttaatcga atgtagtatt cgcggcatgg atatgtttga ttgtgtctta
    10141 ccgacacgta ttgccagaaa tggtacttgt atgacatcgc aaggtcgttt agttattaaa
    10201 aatgcaaaat ttgcagatga tttaagaccg ttagatgaga attgtgactg ttatacatgt
    10261 caaaactatt caagagcgta tatacgtcat ttaatcaagg cagaggaaac ttttggtatt
    10321 cgtcttacta ctattcataa tttacatttt ctgctaaaat taatggaaga tataagaccc
    10381 gggcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    10441 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    10501 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    10561 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    10621 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    10681 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    10741 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    10801 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    10861 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    10921 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    10981 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    11041 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    11101 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    11161 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    11221 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    11281 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    11341 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    11401 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    11461 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    11521 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    11581 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    11641 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    11701 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    11761 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    11821 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    11881 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    11941 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    12001 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    12061 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    12121 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    12181 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    12241 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    12301 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
    12361 cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
    12421 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
    12481 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
    12541 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
    12601 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
    12661 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
    12721 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagcttg
    12781 catgc
//
