LOCUS       sequence-245702-        9097 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Deg-LITer1.0 gene circuit with the following architecture: 
            CMV-TetOx2-TetR-LOV-RRRG---CMV-TetOx2-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9097)
  AUTHORS   Guinn MT, Balazsi G
  TITLE     Noise-reducing optogenetic negative-feedback gene circuits in human 
            cells.
  JOURNAL   Nucleic Acids Res. 2019 Jul 3. pii: 5527975. doi: 
            10.1093/nar/gkz556.
  PUBMED    31269201
REFERENCE   2  (bases 1 to 9097)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2019 Jul 3. pii: 5527975. doi: 10.1093/nar/gkz556."
COMMENT     Sequence Label: pMTG04-AmpFRT
FEATURES             Location/Qualifiers
     source          1..9097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    583..601
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    609..627
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          810..1382
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1394..1403
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1400..1402,1403..1405,1406..2119)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1400..1402,1403..1405,1406..2116)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1445..1466)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1706..1725)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2053..2074
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2566..2790
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        2798..3177
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3178..3381
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    3377..3395
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3403..3421
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     polyA_signal    5055..5279
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5412..5431)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    5563..5610
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5618..6640
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             5618..6640
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    6770..6891
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6807..6826)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6861..6880
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6940..6956)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6940..6956)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6953..6975)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6964..6980
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6988..6994,6995..7012,7013..7018))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7033..7054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7171..7188)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7342..7930)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7422..7441)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8101..8892,8893..8961))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8724..8743
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8962..9066)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag catccctatc agtgatagag
      601 atcagatctc cctatcagtg atagagagct gttttgacct ccatagaaga caccgggacc
      661 gatccagcct ccggactcta gcgtttaaac ttaagcttgg tacccgggga tcctctaggg
      721 cctctgagct attccagaag tagtgaagag gcttttttgg aggcctaggc ttttgcaaaa
      781 agctccggat cgatcctgag aacttcaggg tgagtttggg gacccttgat tgttctttct
      841 ttttcgctat tgtaaaattc atgttatatg gagggggcaa agttttcagg gtgttgttta
      901 gaatgggaag atgtcccttg tatcaccatg gaccctcatg ataattttgt ttctttcact
      961 ttctactctg ttgacaacca ttgtctcctc ttattttctt ttcattttct gtaacttttt
     1021 cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag
     1081 attgtaagta ctttctctaa tcactttttt ttcaaggcaa tcagggtata ttatattgta
     1141 cttcagcaca gttttagaga acaattgtta taattaaatg ataaggtaga atatttctgc
     1201 atataaattc tggctggcgt ggaaatattc ttattggtag aaacaactac atcctggtca
     1261 tcatcctgcc tttctcttta tggttacaat gatatacact gtttgagatg aggataaaat
     1321 actctgagtc caaaccgggc ccctctgcta accatgttca tgccttcttc tttttcctac
     1381 aggtcctgca ggcgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     1441 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     1501 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     1561 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     1621 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     1681 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     1741 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     1801 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     1861 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     1921 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     1981 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     2041 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     2101 ggacgagctg tacaagtaaa ccggtgcggc cgcaatcaac ctctggatta caaaatttgt
     2161 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     2221 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     2281 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     2341 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     2401 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     2461 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     2521 tcctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     2581 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     2641 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     2701 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     2761 ggcatgctgg ggatgcggtg ggctctatgg acgcgttgac attgattatt gactagttat
     2821 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     2881 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     2941 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     3001 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     3061 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     3121 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     3181 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     3241 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     3301 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     3361 gaggtctata taagcatccc tatcagtgat agagatcaga tctccctatc agtgatagag
     3421 agctgttttg acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt
     3481 aaacttaagc ttggtacccg gggatccacc atgggttcta gactggacaa gagcaaagtc
     3541 ataaactctg ctctggaatt actcaatgaa gtcggtatcg aaggcctgac gacaaggaaa
     3601 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
     3661 gccctgctcg atgccctggc aatcgagatg ctggacaggc atcataccca cttctgcccc
     3721 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcatt ccgctgtgct
     3781 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
     3841 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     3901 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggatcag
     3961 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     4021 ctgagacaag caattgagct gttcgaccat cagggagccg aacctgcctt ccttttcggc
     4081 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccaaaa
     4141 aagaagagaa aggctagcgg cgccggcgcc tttttggcta ctacacttga acgtattgag
     4201 aagaactttg tcattactga cccaaggttg ccagataatc ccattatatt cgcgtccgat
     4261 agtttcttgc agttgacaga atatagccgt gaagaaattt tgggaagaaa ctgcaggttt
     4321 ctacaaggtc ctgaaactga tcgcgcgaca gtgagaaaaa ttagagatgc catagataac
     4381 caaacagagg tcactgttca gctgattaat tatacaaaga gtggtaaaaa gttctggaac
     4441 ctctttcact tgcagcctat gcgagatcag aagggagatg tccagtactt tattggggtt
     4501 cagttggatg gaactgagca tgtccgagat gctgccgaga gagagggcgt gatgctggcc
     4561 aagaagaccg ccgagaacat cgacgaggcc gcccgccgcc gcggctaaac cggtgcggcc
     4621 gcaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4681 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4741 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4801 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4861 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4921 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4981 tgttgggcac tgacaattcc gtggtgttgt cctcgagtct agagggcccg tttaaacccg
     5041 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     5101 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     5161 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     5221 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     5281 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     5341 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5401 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5461 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5521 cgaccccaaa aaacttgatt agggtgatgg ttcacgtacc tagaagttcc tattccgaag
     5581 ttcctattct ctagaaagta taggaacttc cttggccaaa aagcctgaac tcaccgcgac
     5641 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     5701 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     5761 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     5821 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     5881 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     5941 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     6001 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     6061 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     6121 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     6181 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     6241 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     6301 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     6361 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     6421 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     6481 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     6541 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     6601 aaaccgacgc cccagcactc gtccgagggc aaaggaatag cacgtactac gagatttcga
     6661 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     6721 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     6781 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6841 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6901 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     6961 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7021 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7081 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     7141 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7201 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7261 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7321 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7381 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7441 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7501 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7561 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7621 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7681 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7741 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     7801 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7861 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7921 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7981 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8041 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     8101 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     8161 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     8221 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     8281 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8341 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8401 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8461 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8521 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8581 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8641 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8701 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8761 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8821 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8881 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8941 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9001 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9061 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
