LOCUS       sequence-24572-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pFlag-CMV-2 converted Gateway Destination vector for expressing Flag
            tagged fusion proteins in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Guo F, Chiang MY, Wang Y, Zhang YZ
  TITLE     An in vitro recombination method to convert restriction- and 
            ligation-independent expression vectors.
  JOURNAL   Biotechnol J. 2008 Mar . 3(3):370-7.
  PUBMED    18064608
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2008 Mar . 3(3):370-7."
COMMENT     Sequence Label: pEZYflag
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..76
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    104..228
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cggggngcnt tagcgagctc ggttngtgaa ccgtcagaat tgatctacca tggactacaa
       61 agacgatgac gacaagcttg cggccgccat gcaattgatt aatacaagtt tgtacaaaaa
      121 agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
      181 aaaacagact acataatact gtaaaacaca acatatccag tcactatgaa tcaactactt
      241 agatggtatt agtgacctgt agtcgaccga cagccttcca aatgttcttc gggtgatgct
      301 gccaacttag tcgaccgaca gccttccaaa tgttcttctc aaacggaatc gtcgtatcca
      361 gcctactcgc tattgtcctc aatgccgtat taaatcataa aaagaaataa gaaaaagagg
      421 tgcgagcctc ttttttgtgt gacaaaataa aaacatctac ctattcatat acgctagtgt
      481 catagtcctg aaaatcatct gcatcaagaa caatttcaca actcttatac ttttctctta
      541 caagtcgttc ggcttcatct ggattttcag cctctatact tactaaacgt gataaagttt
      601 ctgtaatttc tactgtatcg acctgcagac tggctgtgta taagggagcc tgacatttat
      661 attccccaga acatcaggtt aatggcgttt ttgatgtcat tttcgcggtg gctgagatca
      721 gccacttctt ccccgataac ggagaccggc acactggcca tatcgggggt catcatgcgc
      781 c
//
