LOCUS       sequence-245738-        9601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses human PFAS protein with a C-terminal 2XStrep affinity tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9601)
  AUTHORS   Liu C, Knudsen GM, Pedley AM, He J, Johnson JL, Yaron TM, Cantley 
            LC, Benkovic SJ
  TITLE     Mapping Post-Translational Modifications of de Novo Purine 
            Biosynthetic Enzymes: Implications for Pathway Regulation.
  JOURNAL   J Proteome Res. 2019 May 3;18(5):2078-2087. doi: 
            10.1021/acs.jproteome.8b00969. Epub 2019 Apr 18.
  PUBMED    30964683
REFERENCE   2  (bases 1 to 9601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.jproteome.8b00969"; journalName: "J Proteome Res"; 
            date: "2019-05-3- 3"; volume: "18"; issue: "5"; pages: "2078-2087"
COMMENT     Sequence Label: pPFAS-2XStrep
FEATURES             Location/Qualifiers
     source          1..9601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(5165..5170,5171..5194,5195..5230,5231..5254)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     polyA_signal    5305..5426
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5316..5335)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5370..5389
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5612..6067
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5699..5718)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5909..5930
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6184..6541
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6184..6375
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6207..6227)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6392..6527
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6592..7386
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6646..6665)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7256..7275
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7450..7498
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(7539..7558)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7658..7680
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7718..7736)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7804..7908
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7909..7977,7978..8769)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8127..8146)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8940..9528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9429..9448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc ctcgagatgt ccccagtcct tcacttctat gttcgtccct ctggccatga
     1141 gggggcagcc cctggacaca ctcggaggaa actgcaaggg aaactgccag agctgcaggg
     1201 cgtcgagact gaactgtgct acaacgtgaa ctggacagct gaggccctcc ccagtgctga
     1261 ggagacaaag aagctgatgt ggctgtttgg ttgcccctta ctgctggatg atgttgctcg
     1321 ggagtcctgg ctccttcctg gctccaatga cctgctgctg gaggtcgggc ccaggctgaa
     1381 cttctccacc ccaacatcca ccaacatcgt gtcagtgtgc cgcgccactg ggctggggcc
     1441 tgtggatcgt gtggagacca cccggcgcta ccggctctcg tttgcccacc ccccgtcagc
     1501 tgaggtggaa gccattgctc tggctaccct gcacgaccgg atgacagagc agcacttccc
     1561 ccatcccatc cagagtttct cccctgagag catgccggaa cccctcaatg gccctatcaa
     1621 tatactgggt gagggccggc ttgcgctgga gaaggccaac caggagcttg gtctggcttt
     1681 agactcttgg gacctagact tctacaccaa gcgcttccag gagctacagc ggaacccgag
     1741 cactgtggag gcctttgact tggcgcagtc caatagcgag cacagccgac actggttctt
     1801 caagggccag ctccacgtgg atgggcagaa gctggtgcac tcactgtttg agtccatcat
     1861 gagcacccag gaatcctcga accccaacaa cgtcctcaaa ttctgtgata acagcagtgc
     1921 aatccaggga aaggaagtcc gattcctacg gcctgaggac cccacacggc caagccgctt
     1981 ccagcaacag caagggctga gacatgttgt cttcacagca gagactcaca actttcccac
     2041 aggagtatgc ccctttagtg gtgcaaccac tggcacaggg ggccggattc gagatgtcca
     2101 gtgcacaggc cgcggggccc acgtggtggc tggcactgcc ggctattgct ttggaaatct
     2161 gcatattcca ggttacaatc tgccctggga ggatccaagc ttccagtatc ctgggaattt
     2221 tgcccggccc ctggaggttg ccattgaagc cagtaatgga gcttctgact atggcaacaa
     2281 gtttggggaa ccagtgctgg ctggcttcgc ccgctccttg ggcctccagc tcccagacgg
     2341 ccagcggcgt gagtggatca agcccatcat gtttagtggg ggcattgggt ccatggaagc
     2401 tgaccacata agcaaggagg ccccagagcc aggcatggaa gttgtaaagg ttggaggtcc
     2461 cgtctacagg attggagttg gaggtggagc tgcttcatct gtgcaggtgc agggagataa
     2521 caccagtgac ctggactttg gggctgtgca gcgaggagac ccggagatgg aacagaagat
     2581 gaaccgtgtg atcagggctt gtgtggaggc ccccaaggga aaccccatct gcagccttca
     2641 tgatcagggc gctggtggca atggcaatgt cctaaaagag ctgagtgacc cagctggagc
     2701 catcatttac accagccgct tccagcttgg ggacccaacc ctgaatgccc tggaaatctg
     2761 gggggctgag taccaggaat caaatgctct tctgctgagg tcccccaacc gggacttcct
     2821 gactcatgtc agtgcccgtg aacgttgccc ggcttgcttc gtgggcacca tcactggaga
     2881 ccggagaata gtgctggtgg acgatcggga gtgtcctgtc agaagaaatg gccaggggga
     2941 tgcccccccg acacccctgc caacccctgt ggacctggag ctcgaatggg tgctgggcaa
     3001 gatgcctcgg aaggagttct tcctgcagag gaagcccccc atgctgcagc ctctggcctt
     3061 gcccccaggg ctgagcgtgc accaggctct ggagagggtt ctgaggctgc ccgccgtggc
     3121 cagcaagcgc tacctcacca ataaggtgga ccgctccgtg ggaggcctgg tggcccagca
     3181 gcagtgcgtg gggcccctgc aaactcctct ggcagatgta gcggttgtgg cactgagcca
     3241 tgaggagctc ataggggctg ccacagcctt gggagaacag ccagtcaaga gcctgctgga
     3301 cccaaaagtc gccgcccggc tggccgtggc cgaagccctc accaacctgg tgtttgctct
     3361 ggtcactgac ctccgggatg tgaagtgtag cgggaactgg atgtgggcag ccaagctccc
     3421 aggggagggc gcagctttgg cggatgcctg tgaggctatg gtggcagtga tggcagccct
     3481 gggtgtggca gtggatggtg gcaaggactc cctcagcatg gctgctcggg ttggcactga
     3541 gaccgtgcgg gctcctgggt cactggtcat ctcagcctat gccgtctgcc cagacatcac
     3601 agccactgtg accccagacc tcaagcatcc tgaagggaga ggccatctgc tctatgtggc
     3661 tctgagccct gggcagcacc ggctcggggg cacagctctg gcccagtgct tctcccagct
     3721 tggggaacac cctccagacc tggaccttcc tgagaacttg gtgcgggcct tcagcatcac
     3781 tcaggggctg ctgaaagacc gcctcctctg ctcaggccac gatgtcagtg acggaggcct
     3841 cgtcacatgc ctgctggaga tggcctttgc tggaaattgc gggctacagg tggatgtgcc
     3901 tgtccccagg gttgatgtcc tgtctgtgct gttcgctgag gagccaggcc tcgtgctgga
     3961 ggtgcaggag ccagacctgg cccaggtgct gaagcgttac cgggatgctg gcctccattg
     4021 cctggagctg ggccacacag gcgaggccgg gccccacgcc atggtccggg tgtcagtgaa
     4081 cggggctgtg gttctggagg agcctgttgg ggagctgcga gccctctggg aggagacgag
     4141 tttccagctg gaccggctac aggcagagcc tcgctgtgtg gcagaggagg aacggggcct
     4201 gagggagcgg atggggccca gctattgcct gccccccacc tttcccaaag cctccgtgcc
     4261 ccgtgagcct ggtggtccca gcccccgagt cgccatcttg cgagaggagg gcagtaatgg
     4321 agaccgggag atggccgatg ccttccactt agctgggttt gaggtatggg acgtgaccat
     4381 gcaggacctc tgctctgggg caattgggct ggacactttc cgtggcgtgg ccttcgtggg
     4441 cggcttcagc tatgcagatg tcctgggctc tgccaaaggg tgggcagctg ctgtgacctt
     4501 tcatcccagg gctggggctg agctgaggcg cttccggaag cggccagaca ccttcagcct
     4561 gggcgtgtgt aatggctgtc aactgctggc tctgctcggc tgggtgggag gcgaccccaa
     4621 tgaggatgct gcagagatgg gccctgactc ccagccagcc cggccaggcc ttctgctacg
     4681 ccacaacctg tctgggcgct acgagtctcg ctgggccagc gtgcgtgtgg ggcctgggcc
     4741 agccctgatg ctgcgaggga tggagggcgc cgtgctgccc gtgtggagtg cgcacgggga
     4801 aggttacgta gcattttctt ctccggaact ccaagctcag attgaggcca ggggcttggc
     4861 tccactgcac tgggctgatg atgacgggaa ccccacagag cagtaccctc tgaatcccaa
     4921 tgggtcccca gggggcgtgg ctggcatctg ctcctgtgat ggccgccacc tggctgtcat
     4981 gcctcaccct gagcgggccg ttaggccttg gcagtgggca tggcgacccc ctccatttga
     5041 tactctgacc acctccccct ggctccagct ctttatcaat gcccgaaact ggaccctgga
     5101 agggagctgc cgaattctgc agtcgacggt accgcgggcc cgggatccac cggtcgccac
     5161 cgggagcgct tggagccacc cgcagttcga aaaaggtgga ggttctggcg gtggatcggg
     5221 aggttcagcg tggagccacc cgcagttcga gaaataaagc ggccgcttcc ctttagtgag
     5281 ggttaatgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
     5341 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     5401 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     5461 ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
     5521 tccgataagg atcgatccgg gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     5581 ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg
     5641 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5701 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5761 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5821 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5881 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5941 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6001 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     6061 acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     6121 gcggatctgc gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttaggtac
     6181 cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     6241 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     6301 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6361 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6421 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6481 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6541 aaagcttgat tcttctgaca caacagtctc gaacttaagg ctagagccac catgattgaa
     6601 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     6661 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     6721 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     6781 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     6841 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     6901 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     6961 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     7021 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     7081 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     7141 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     7201 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     7261 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     7321 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     7381 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     7441 gatggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa
     7501 tcgatagcga taaggatccg cgtatggtgc actctcagta caatctgctc tgatgccgca
     7561 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     7621 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     7681 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     7741 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     7801 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     7861 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     7921 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     7981 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     8041 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     8101 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     8161 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     8221 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     8281 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     8341 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     8401 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     8461 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     8521 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     8581 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     8641 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     8701 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     8761 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     8821 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     8881 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     8941 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9001 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9061 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     9121 aagaactctg tagcactgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     9181 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     9241 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     9301 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     9361 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     9421 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     9481 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     9541 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatggc tcgacagatc
     9601 t
//
